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Biochemical Large-Scale Identification of MHC Class I Ligands | SpringerLink
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The large-scale identification of MHC class I presented peptides is indispensable for gaining insight into the fundamental rules of immune recognition as well as it is an invaluable tool in identifying potential targets for the immunotherapy of disease. In this...
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Keywords {🔍}
pubmed, article, google, scholar, class, cas, mhc, protocol, identification, peptides, stevanovic, privacy, cookies, content, information, publish, ligands, chapter, cell, pii, rammensee, monoclonal, analysis, processing, search, largescale, stevanović, biology, presented, purification, cells, molecules, immunol, hla, nature, download, tübingen, springer, usd, personal, data, log, journal, research, antigen, kowalewski, stefan, book, access, isolation,
Topics {✒️}
biochemical large-scale identification hla-dr-bound peptides presented month download article/chapter mass spectrometry-based phosphoproteomics allele-specific motifs revealed cell activation–peptide generation large-scale identification class i-presented peptides large-scale production privacy choices/manage cookies device instant download hla class i editor information editors faculté de médecine antigen processing mild acid elution european economic area identifying potential targets zeh hj 3rd h-2kb molecule journal finder publish t-cell epitopes cell-independent approach conditions privacy policy van endert cytotoxic monoclonal antibody accepting optional cookies immunobead purification technique université paris descartes targeted mhc class main content log author correspondence protocol usd 49 peptide motifs social media permissions reprints protocol kowalewski monoclonal antibodies protocol cite bound peptides affiliations department humana press presented peptides ligands protocol hla class depth protocol journal publish privacy policy check access ethics access
Schema {🗺️}
ScholarlyArticle:
headline:Biochemical Large-Scale Identification of MHC Class I Ligands
pageEnd:157
pageStart:145
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-218-6.jpg
genre:
Springer Protocols
isPartOf:
name:Antigen Processing
isbn:
978-1-62703-218-6
978-1-62703-217-9
type:Book
publisher:
name:Humana Press
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Daniel J. Kowalewski
affiliation:
name:Interfaculty Institute for Cell Biology, University of Tübingen
address:
name:Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany
type:PostalAddress
type:Organization
type:Person
name:Stefan Stevanović
affiliation:
name:University of Tübingen, Interfaculty Institute for Cell Biology
address:
name:Department of Immunology, University of Tübingen, Interfaculty Institute for Cell Biology, Tübingen, Germany
type:PostalAddress
type:Organization
email:[email protected]
type:Person
keywords:MHC class I, pMHC, Antigen presentation, Ligandome analysis, Immunoaffinity purification, Mass spectrometry
description:The large-scale identification of MHC class I presented peptides is indispensable for gaining insight into the fundamental rules of immune recognition as well as it is an invaluable tool in identifying potential targets for the immunotherapy of disease. In this chapter we briefly review the existing strategies for the analysis of MHC ligandomes and provide an in-depth protocol for the immunoaffinity purification of MHC class I presented peptides from primary tissues or cells.
datePublished:2013
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Book:
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isbn:
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name:Humana Press
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name:Interfaculty Institute for Cell Biology, University of Tübingen
address:
name:Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany
type:PostalAddress
name:University of Tübingen, Interfaculty Institute for Cell Biology
address:
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Person:
name:Daniel J. Kowalewski
affiliation:
name:Interfaculty Institute for Cell Biology, University of Tübingen
address:
name:Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany
type:PostalAddress
type:Organization
name:Stefan Stevanović
affiliation:
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address:
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type:PostalAddress
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email:[email protected]
PostalAddress:
name:Department of Immunology, Interfaculty Institute for Cell Biology, University of Tübingen, Tübingen, Germany
name:Department of Immunology, University of Tübingen, Interfaculty Institute for Cell Biology, Tübingen, Germany
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