We are analyzing https://link.springer.com/protocol/10.1007/978-1-62703-125-7_19.

Title:
Primary Mouse Small Intestinal Epithelial Cell Cultures | SpringerLink
Description:
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently shown that Lgr5 (Leucine-rich repeat-containing G protein-coupled receptor) is expressed in intestinal stem cells by an in vivo genetic lineage tracing strategy. In...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Primary Mouse Small Intestinal Epithelial Cell Cultures
      pageEnd:328
      pageStart:319
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-125-7.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Epithelial Cell Culture Protocols
         isbn:
            978-1-62703-125-7
            978-1-62703-124-0
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Toshiro Sato
            affiliation:
                  name:Keio University
                  address:
                     name:Department of Gastroenterology, School of Medicine, Keio University, Tokyo, Japan
                     type:PostalAddress
                  type:Organization
                  name:Hubrecht Institute
                  address:
                     name:Hubrecht Institute, Utrecht, The Netherlands
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Hans Clevers
            affiliation:
                  name:Hubrecht Institute
                  address:
                     name:Hubrecht Institute, Utrecht, The Netherlands
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Small intestinal crypts, Intestinal stem cells, Wnt signal, Lgr5, Flow cytometry, Confocal immunofluorescence
      description:The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently shown that Lgr5 (Leucine-rich repeat-containing G protein-coupled receptor) is expressed in intestinal stem cells by an in vivo genetic lineage tracing strategy. In the past, extensive efforts have been made to establish primary small intestinal culture systems. However, no defined, reproducible and robust culture system had been developed. To establish such a system, we screened for optimal growth factor combinations based on genetic evidence of self-renewal regulation, differentiation, and carcinogenesis of intestinal stem cells. Here, we describe methods that we have established for the isolation and culture of primary small intestinal epithelial stem cells. In this culture system, isolated crypts form “organoid structures” with a histological hierarchy recapitulating in vivo small intestinal epithelium. Single isolated Lgr5+ intestinal stem cells also form these organoid structures, in which stem cells are maintained by self-renewal and give rise to all lineages of the intestinal epithelium. This culture system is particularly useful for studying the regulation of intestinal stem cell self-renewal and differentiation.
      datePublished:2012
      isAccessibleForFree:
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Book:
      name:Epithelial Cell Culture Protocols
      isbn:
         978-1-62703-125-7
         978-1-62703-124-0
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Keio University
      address:
         name:Department of Gastroenterology, School of Medicine, Keio University, Tokyo, Japan
         type:PostalAddress
      name:Hubrecht Institute
      address:
         name:Hubrecht Institute, Utrecht, The Netherlands
         type:PostalAddress
      name:Hubrecht Institute
      address:
         name:Hubrecht Institute, Utrecht, The Netherlands
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Toshiro Sato
      affiliation:
            name:Keio University
            address:
               name:Department of Gastroenterology, School of Medicine, Keio University, Tokyo, Japan
               type:PostalAddress
            type:Organization
            name:Hubrecht Institute
            address:
               name:Hubrecht Institute, Utrecht, The Netherlands
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Hans Clevers
      affiliation:
            name:Hubrecht Institute
            address:
               name:Hubrecht Institute, Utrecht, The Netherlands
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Gastroenterology, School of Medicine, Keio University, Tokyo, Japan
      name:Hubrecht Institute, Utrecht, The Netherlands
      name:Hubrecht Institute, Utrecht, The Netherlands
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      isAccessibleForFree:
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