We are analyzing https://link.springer.com/protocol/10.1007/978-1-62703-038-0_33.

Title:
Isolating mRNA and Small Noncoding RNAs from Human Sperm | SpringerLink
Description:
The isolation of spermatozoal RNA is a challenging procedure due to the intrinsic heterogeneous population of cells present in the ejaculate and the small quantity of RNA present in sperm. The transcriptome of these gametes includes a wide variety of messenger RNAs...
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Keywords {🔍}

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Topics {✒️}

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Questions {❓}

Lewis SE (2007) Is sperm evaluation useful in predicting human fertility?

Schema {🗺️}

ScholarlyArticle:
      headline:Isolating mRNA and Small Noncoding RNAs from Human Sperm
      pageEnd:396
      pageStart:385
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-62703-038-0.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Spermatogenesis
         isbn:
            978-1-62703-038-0
            978-1-62703-037-3
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Robert J. Goodrich
            affiliation:
                  name:Wayne State University School of Medicine
                  address:
                     name:Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ester Anton
            affiliation:
                  name:Wayne State University School of Medicine
                  address:
                     name:Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, C.S. Mott Center for Human Growth and Development, Detroit, Wayne State University School of Medicine, Michigan, USA
                     type:PostalAddress
                  type:Organization
                  name:Unitat de Biologia Cellular, Universitat Autònoma de Barcelona
                  address:
                     name:Unitat de Biologia Cellular, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Stephen A. Krawetz
            affiliation:
                  name:Wayne State University School of Medicine
                  address:
                     name:Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Human spermatozoa, PureSperm gradient, mRNA and sncRNA extraction, RNeasy Mini kit, RNeasy Minelute kit, QIAcube
      description:The isolation of spermatozoal RNA is a challenging procedure due to the intrinsic heterogeneous population of cells present in the ejaculate and the small quantity of RNA present in sperm. The transcriptome of these gametes includes a wide variety of messenger RNAs (mRNAs), small noncoding RNAs (sncRNAs), and highly fragmented ribosomal RNAs (rRNAs). The protocol described in this chapter to isolate both the mRNA and sncRNA fractions represents years of development towards automation. It combines a guanidinium thiocyanate–phenol–chloroform-based methodology to reduce the content of DNA and a column-based system. Both manual and semi-automated options are described, with preference given to automation for consistent results. A novel quality control procedure has been developed to assess the integrity and purity of the entire population of isolated mRNAs due to the absence of intact rRNAs.
      datePublished:2013
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      isbn:
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         978-1-62703-037-3
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Wayne State University School of Medicine
      address:
         name:Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, USA
         type:PostalAddress
      name:Wayne State University School of Medicine
      address:
         name:Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, C.S. Mott Center for Human Growth and Development, Detroit, Wayne State University School of Medicine, Michigan, USA
         type:PostalAddress
      name:Unitat de Biologia Cellular, Universitat Autònoma de Barcelona
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         name:Unitat de Biologia Cellular, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain
         type:PostalAddress
      name:Wayne State University School of Medicine
      address:
         name:Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, USA
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Person:
      name:Robert J. Goodrich
      affiliation:
            name:Wayne State University School of Medicine
            address:
               name:Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, USA
               type:PostalAddress
            type:Organization
      name:Ester Anton
      affiliation:
            name:Wayne State University School of Medicine
            address:
               name:Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, C.S. Mott Center for Human Growth and Development, Detroit, Wayne State University School of Medicine, Michigan, USA
               type:PostalAddress
            type:Organization
            name:Unitat de Biologia Cellular, Universitat Autònoma de Barcelona
            address:
               name:Unitat de Biologia Cellular, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain
               type:PostalAddress
            type:Organization
      name:Stephen A. Krawetz
      affiliation:
            name:Wayne State University School of Medicine
            address:
               name:Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Obstetrics and Gynecology, C. S. Mott Center for Human Growth and Development, Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, USA
      name:Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, C.S. Mott Center for Human Growth and Development, Detroit, Wayne State University School of Medicine, Michigan, USA
      name:Unitat de Biologia Cellular, Universitat Autònoma de Barcelona, Bellaterra (Cerdanyola del Vallès), Spain
      name:Department of Obstetrics and Gynecology, Center for Molecular Medicine and Genetics, C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, USA
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