We are analyzing https://link.springer.com/protocol/10.1007/978-1-61779-207-6_28.

Title:
Cell Migration in Confinement: A Micro-Channel-Based Assay | SpringerLink
Description:
This chapter describes a method to study cells migrating in micro-channels, a confining environment of well-defined geometry. This assay is a complement to more complex 3D migration systems and provides several advantages even if it does not recapitulate the full...
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Schema {🗺️}

ScholarlyArticle:
      headline:Cell Migration in Confinement: A Micro-Channel-Based Assay
      pageEnd:434
      pageStart:415
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-61779-207-6.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Cell Migration
         isbn:
            978-1-61779-207-6
            978-1-61779-206-9
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Mélina L. Heuzé
            affiliation:
            type:Person
            name:Olivier Collin
            affiliation:
            type:Person
            name:Emmanuel Terriac
            affiliation:
            type:Person
            name:Ana-Maria Lennon-Duménil
            affiliation:
            type:Person
            name:Matthieu Piel
            affiliation:
                  name:Institut Curie
                  address:
                     name:Subcellular Structure and Cellular Dynamics, Institut Curie, Paris, Cedex, France
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Micro-fabrication, Micro-channels, Cell migration
      description:This chapter describes a method to study cells migrating in micro-channels, a confining environment of well-defined geometry. This assay is a complement to more complex 3D migration systems and provides several advantages even if it does not recapitulate the full complexity of 3D migration. Important parameters such as degree of adhesion, degree of confinement, mechanical properties, and geometry can be varied independently of each other. The device is fully compatible with almost any type of light microscopy and the simple geometry makes automated analysis very easy to perform, which allows screening strategy. The chapters is divided into five parts describing the design of different types of migration chambers, the fabrication of a mold by photolithography, the assembly of the chamber, the loading of cells, and finally the imaging on live or fixed cells.
      datePublished:2011
      isAccessibleForFree:
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         type:WebPageElement
      context:https://schema.org
Book:
      name:Cell Migration
      isbn:
         978-1-61779-207-6
         978-1-61779-206-9
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Institut Curie
      address:
         name:Subcellular Structure and Cellular Dynamics, Institut Curie, Paris, Cedex, France
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Mélina L. Heuzé
      affiliation:
      name:Olivier Collin
      affiliation:
      name:Emmanuel Terriac
      affiliation:
      name:Ana-Maria Lennon-Duménil
      affiliation:
      name:Matthieu Piel
      affiliation:
            name:Institut Curie
            address:
               name:Subcellular Structure and Cellular Dynamics, Institut Curie, Paris, Cedex, France
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Subcellular Structure and Cellular Dynamics, Institut Curie, Paris, Cedex, France
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

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