We are analyzing https://link.springer.com/protocol/10.1007/978-1-60761-753-2_15.

Title:
A Rapid and General Assay for Monitoring Endogenous Gene Modification | SpringerLink
Description:
The development of zinc finger nucleases for targeted gene modification can benefit from rapid functional assays that directly quantify activity at the endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand...
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28 years and 1 months (reg. 1997-05-29).

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Keywords {🔍}

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Topics {✒️}

engineered zinc-finger nucleases custom dna-binding domains rapid “open-source” engineering designed zinc-finger nucleases customized zinc-finger nucleases month download article/chapter high-frequency homologous recombination zinc finger nucleases zinc-finger nucleases efficient gene targeting zinc finger tools zinc finger targeter privacy choices/manage cookies targeted gene modification device instant download enhancing gene targeting targeted gene inactivation targeted gene addition targeted gene knockout author information authors homologous end joining targeted transgene integration targeted chromosomal cleavage european economic area directly quantify activity wild-type sequence nucleic acids res rare-cutting endonucleases journal finder publish conditions privacy policy human stem cells rapid functional assays accepting optional cookies endogenous target protocol usd 49 plant mol biol main content log methods mol biol usa dmitry protocol cite gene editing targeted disruption social media check access permissions reprints ethics access surveyor nuclease genome editing xiangdong meng humana press

Schema {🗺️}

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      headline:A Rapid and General Assay for Monitoring Endogenous Gene Modification
      pageEnd:256
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      genre:
         Springer Protocols
      isPartOf:
         name:Engineered Zinc Finger Proteins
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            978-1-60761-752-5
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         name:Humana Press
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      author:
            name:Dmitry Y. Guschin
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      keywords:Zinc finger nuclease (ZFN), designed zinc finger proteins, non-homologous end joining (NHEJ), Surveyor nuclease, Cel 1, genome modification
      description:The development of zinc finger nucleases for targeted gene modification can benefit from rapid functional assays that directly quantify activity at the endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand break repair via non-homologous end joining. The assay is based on the ability of the Surveyor nuclease to selectively cleave distorted duplex DNA formed via cross-annealing of mutated and wild-type sequence.
      datePublished:2010
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