We are analyzing https://link.springer.com/protocol/10.1007/978-1-60327-977-2_19.

Title:
In Toto Imaging of Embryogenesis with Confocal Time-Lapse Microscopy | SpringerLink
Description:
Microscopy has been one of the most direct and powerful tools since the beginning of biological research. Continued advances such as confocal and two-photon fluorescence microscopy and fluorescent proteins now make imaging useful at a variety of spatial scales...
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Keywords {🔍}

imaging, protocol, microscopy, article, toto, megason, biology, cell, privacy, cookies, content, data, information, publish, research, zebrafish, access, search, download, springer, usd, personal, log, journal, track, confocal, sean, book, methods, molecular, biological, chapter, developmental, open, cas, pubmed, google, scholar, nature, author, press, ebook, make, optional, analysis, media, parties, policy, find, embryogenesis,

Topics {✒️}

high-performance laser-scanning microscopy month download article/chapter confocal time-lapse microscopy springer science+business media privacy choices/manage cookies device instant download humana press photon fluorescence microscopy selected microscopy techniques editor information editors protocol zebrafish sean journal finder publish european economic area single cell movement conditions privacy policy continued technological improvements harvard medical school royal melbourne hospital accepting optional cookies biological research choosing fluorescent proteins molecular & developmental single cell level check access ethics access main content log protocol megason online cell lineage author correspondence medical research protocol usd 49 journal publish protocol cite nature methods 2 privacy policy megason part affiliations department national institute permissions reprints books a molecular biology systems biology optional cookies social media manage preferences fluorescent proteins chapter presents chapter log personal data

Schema {🗺️}

ScholarlyArticle:
      headline:In Toto Imaging of Embryogenesis with Confocal Time-Lapse Microscopy
      pageEnd:332
      pageStart:317
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-60327-977-2.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Zebrafish
         isbn:
            978-1-60327-977-2
            978-1-60327-976-5
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Sean G. Megason
            affiliation:
                  name:Harvard Medical School
                  address:
                     name:Department of Systems Biology, Harvard Medical School, Boston, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Confocal microscopy, Two-photon, In toto imaging, In vivo, Time lapse, Zebrafish, Image Analysis, Fluorescent, GFP, Live cell imaging
      description:Microscopy has been one of the most direct and powerful tools since the beginning of biological research. Continued advances such as confocal and two-photon fluorescence microscopy and fluorescent proteins now make imaging useful at a variety of spatial scales (molecules, circuits, cells, tissues, and even whole embryos) and temporal scales (<seconds to days). Zebrafish is uniquely poised to benefit from these continued technological improvements because of its inherent suitability for both imaging and genetics. This chapter presents an approach called “in toto imaging”. The goal of in toto imaging is to image and track every single cell movement and division that forms a tissue or organ. This approach is powerful for understanding how cell lineage, shape changes, and movements control the morphogenesis of a tissue. When used with transgenic lines, in toto imaging can be used to “digitize” data at single cell level over time from a living organism. This quantitative, digitized data can then serve as the basis for forming models of how biological circuits orchestrate developmental processes.
      datePublished:2009
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:Zebrafish
      isbn:
         978-1-60327-977-2
         978-1-60327-976-5
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Harvard Medical School
      address:
         name:Department of Systems Biology, Harvard Medical School, Boston, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Sean G. Megason
      affiliation:
            name:Harvard Medical School
            address:
               name:Department of Systems Biology, Harvard Medical School, Boston, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Systems Biology, Harvard Medical School, Boston, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

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