We are analyzing https://link.springer.com/protocol/10.1007/978-1-60327-461-6_19.

Title:
Single Molecule Tracking for Studying Nucleocytoplasmic Transport and Intranuclear Dynamics | SpringerLink
Description:
Microscopic imaging of single fluorescent molecules within cells provides a molecular, real-time view of physiological processes in vivo. Single fluorescent molecules produce diffraction-limited light spots in the image plane, which can be localised with a very high...
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Keywords {🔍}

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Topics {✒️}

wide-field fluorescence imaging single-molecule fluorescence microscopy nonlinear structured-illumination microscopy narrow-field epifluorescence microscopy single fluorescent molecules single-molecule tracking single molecule tracking privacy choices/manage cookies high-speed imaging study intranuclear protein single molecule level single-photon microscopy high time resolution real-time view theoretically unlimited resolution high localisation precision european economic area brownian motion characterised bimolecular interaction events nuclear pore complex nuclear pore complexes nuclear protein import intranuclear binding kinetics journal finder publish conditions privacy policy fluorescence microscopy accepting optional cookies studying nucleocytoplasmic transport highly dynamic processes eukaryotic cell nuclei chapter log single molecules protocol siebrasse ribonucleoprotein particle mobility main content log check access ethics access binding site distribution humana press intranuclear dynamics soluble receptors protocol cite high precision journal publish privacy policy personal data permissions reprints books a information nuclear transport

Schema {🗺️}

ScholarlyArticle:
      headline:Single Molecule Tracking for Studying Nucleocytoplasmic Transport and Intranuclear Dynamics
      pageEnd:361
      pageStart:343
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-60327-461-6.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:The Nucleus
         isbn:
            978-1-60327-461-6
            978-1-60327-460-9
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Jan Peter Siebrasse
            affiliation:
                  name:University of Bonn
                  address:
                     name:Institute for Physical and Theoretical Chemistry, Department of Biophysical Chemistry, University of Bonn, Bonn, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Ulrich Kubitscheck
            affiliation:
                  name:University of Bonn
                  address:
                     name:Institute for Physical and Theoretical Chemistry, Department of Biophysical Chemistry, University of Bonn, Bonn, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Single-molecule fluorescence microscopy, Single-molecule tracking, Light microscopy, Nucleocytoplasmic transport, Fluorescence labelling
      description:Microscopic imaging of single fluorescent molecules within cells provides a molecular, real-time view of physiological processes in vivo. Single fluorescent molecules produce diffraction-limited light spots in the image plane, which can be localised with a very high precision. In single-molecule fluorescence microscopy (SMF) the achievable localisation precision depends only on the signal-to-noise ratio (SNR) and the stability of the optical setup. Typically values between 20 and 40nm can be achieved. Highly dynamic processes and Brownian motion characterised by diffusion coefficients <20μm2/sec can be followed by high-speed imaging, hence the method is an ideal tool to study intranuclear protein or ribonucleoprotein particle mobility. In contrast to conventional techniques, different forms of mobility in a heterogeneous system may well be distinguished from each other. Furthermore, specific binding and bimolecular interaction events can be followed at the single molecule level. A prominent example of an application is the study of nucleocytoplasmic transport one molecule at a time. In this case, the high localisation precision allows to analyse the binding site distribution of single molecules at the nuclear pore complex, and the high time resolution allows determination of the binding duration of soluble receptors and transport substrates.
      datePublished:2008
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Book:
      name:The Nucleus
      isbn:
         978-1-60327-461-6
         978-1-60327-460-9
Organization:
      name:Humana Press
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         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Bonn
      address:
         name:Institute for Physical and Theoretical Chemistry, Department of Biophysical Chemistry, University of Bonn, Bonn, Germany
         type:PostalAddress
      name:University of Bonn
      address:
         name:Institute for Physical and Theoretical Chemistry, Department of Biophysical Chemistry, University of Bonn, Bonn, Germany
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Jan Peter Siebrasse
      affiliation:
            name:University of Bonn
            address:
               name:Institute for Physical and Theoretical Chemistry, Department of Biophysical Chemistry, University of Bonn, Bonn, Germany
               type:PostalAddress
            type:Organization
      name:Ulrich Kubitscheck
      affiliation:
            name:University of Bonn
            address:
               name:Institute for Physical and Theoretical Chemistry, Department of Biophysical Chemistry, University of Bonn, Bonn, Germany
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Institute for Physical and Theoretical Chemistry, Department of Biophysical Chemistry, University of Bonn, Bonn, Germany
      name:Institute for Physical and Theoretical Chemistry, Department of Biophysical Chemistry, University of Bonn, Bonn, Germany
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