We are analyzing https://link.springer.com/protocol/10.1007/978-1-60327-299-5_16.

Title:
Zymography and Reverse Zymography for Detecting MMPs and TIMPs | SpringerLink
Description:
Zymography is the electrophoretic separation of proteins through a polyacrylamide gel containing a proteolytic substrate. After denaturing (but nonreducing) electrophoresis, proteins are renatured and incubated in an appropriate buffer for proteolytic activity. Clear...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Zymography and Reverse Zymography for Detecting MMPs and TIMPs
      pageEnd:269
      pageStart:257
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-60327-299-5.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Matrix Metalloproteinase Protocols
         isbn:
            978-1-60327-299-5
            978-1-60327-298-8
         type:Book
      publisher:
         name:Humana Press
         logo:
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            type:ImageObject
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      author:
            name:Susan P. Hawkes
            affiliation:
                  name:University of California
                  address:
                     name:Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California, San Francisco, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hongxia Li
            affiliation:
                  name:University of California
                  address:
                     name:Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California, San Francisco, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Gary T. Taniguchi
            affiliation:
                  name:University of California
                  address:
                     name:Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California, San Francisco, USA
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Electrophoresis, substrate, gel, activity
      description:Zymography is the electrophoretic separation of proteins through a polyacrylamide gel containing a proteolytic substrate. After denaturing (but nonreducing) electrophoresis, proteins are renatured and incubated in an appropriate buffer for proteolytic activity. Clear zones of lysis in the stained gel indicated active proteinases. Reverse zymography is a similar technique to detect proteinase inhibitors. After renaturing of proteins, the gel is incubated with metalloproteinases which digest the substrate incorporated into the gel. Inhibitors are shown as dark zones of inhibition against a clear background upon staining.
      datePublished:2010
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Book:
      name:Matrix Metalloproteinase Protocols
      isbn:
         978-1-60327-299-5
         978-1-60327-298-8
Organization:
      name:Humana Press
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      name:University of California
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            address:
               name:Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California, San Francisco, USA
               type:PostalAddress
            type:Organization
      name:Hongxia Li
      affiliation:
            name:University of California
            address:
               name:Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California, San Francisco, USA
               type:PostalAddress
            type:Organization
      name:Gary T. Taniguchi
      affiliation:
            name:University of California
            address:
               name:Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California, San Francisco, USA
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California, San Francisco, USA
      name:Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California, San Francisco, USA
      name:Departments of Biopharmaceutical Sciences and Pharmaceutical Chemistry, University of California, San Francisco, USA
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