We are analyzing https://link.springer.com/protocol/10.1007/978-1-60327-058-8_12.

Title:
Application of High-Throughput Methodologies to the Expression of Recombinant Proteins in E. coli | SpringerLink
Description:
Despite the large body of knowledge accumulated on recombinant protein expression, production, primarily of eukaryotic proteins, remains a challenge. The biggest obstacle is in obtaining large amounts of a given protein in a correctly folded form. Several strategies...
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Keywords {🔍}

expression, proteins, protein, molecular, google, scholar, structural, cas, proteomics, peleg, privacy, cookies, content, information, publish, protocol, recombinant, unger, screening, israel, acta, cryst, research, search, highthroughput, methodologies, coli, methods, biology, solubility, chapter, sussman, data, log, journal, eukaryotic, multiple, access, article, ispc, busso, authors, school, microbial, university, queensland, australia, press, springer, optional,

Topics {✒️}

economical medium-throughput strategy springer science+business media molecular chaperone-mediated folding high-throughput cloning methods privacy choices/manage cookies high-throughput expression screening european economic area humana press high-throughput methodologies journal finder publish structural proteomics author information authors correctly folded form identify clones capable 3d structure determination current protocols colony filtration blot conditions privacy policy producing soluble proteins obtaining large amounts zeev-ben-mordehai protocol peleg accepting optional cookies protocol cite main content log multiple expression vectors recombinant protein expression soluble protein expression affiliations school structural biology journal publish israel ministry protein complexes chapter log recombinant proteins privacy policy israel silman personal data check access ethics access permissions reprints books a optional cookies social media manage preferences rescue strategy dna cloning multiple proteins data protection essential cookies

Schema {🗺️}

ScholarlyArticle:
      headline:Application of High-Throughput Methodologies to the Expression of Recombinant Proteins in E. coli
      pageEnd:208
      pageStart:197
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-60327-058-8.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Structural Proteomics
         isbn:
            978-1-60327-058-8
            978-1-58829-809-6
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Yoav Peleg
            affiliation:
                  name:Weizmann Institute of Science
                  address:
                     name:Israel Structural Proteomics Center (ISPC), Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Tamar Unger
            affiliation:
                  name:Weizmann Institute of Science
                  address:
                     name:Israel Structural Proteomics Center (ISPC), Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Tobacco Etch Virus, Soluble Recombinant Protein, Tobacco Etch Virus Cleavage, Durapore Membrane, Parallel Cloning
      description:Despite the large body of knowledge accumulated on recombinant protein expression, production, primarily of eukaryotic proteins, remains a challenge. The biggest obstacle is in obtaining large amounts of a given protein in a correctly folded form. Several strategies are being used to increase both yields and solubility. These include expression with fusion proteins, co-expression with molecular chaperones or a protein partner, and use of multiple constructs for each protein. Any given method may help to increase expression and solubility for a given protein, but often more than one rescue strategy should be tried. To perform several different rescue strategies on multiple proteins, high throughout (HTP) methodologies are applied. This chapter presents HTP methodologies for DNA cloning in multiple expression vectors and expression screening to identify clones capable of producing soluble proteins.
      datePublished:2008
      isAccessibleForFree:
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         type:WebPageElement
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Book:
      name:Structural Proteomics
      isbn:
         978-1-60327-058-8
         978-1-58829-809-6
Organization:
      name:Humana Press
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Weizmann Institute of Science
      address:
         name:Israel Structural Proteomics Center (ISPC), Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
         type:PostalAddress
      name:Weizmann Institute of Science
      address:
         name:Israel Structural Proteomics Center (ISPC), Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Yoav Peleg
      affiliation:
            name:Weizmann Institute of Science
            address:
               name:Israel Structural Proteomics Center (ISPC), Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
               type:PostalAddress
            type:Organization
      name:Tamar Unger
      affiliation:
            name:Weizmann Institute of Science
            address:
               name:Israel Structural Proteomics Center (ISPC), Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Israel Structural Proteomics Center (ISPC), Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
      name:Israel Structural Proteomics Center (ISPC), Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel
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