We are analyzing https://link.springer.com/protocol/10.1007/978-1-59745-204-5_21.

Title:
Fungal and Bacterial Killing by Neutrophils | SpringerLink
Description:
Neutrophils are professional phagocytes of the innate immune system that are essential to control bacterial and fungal infections. These cells engulf and kill invading microbes. Additionally, activated neutrophils are able to release neutrophil extracellular traps...
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

ScholarlyArticle:
      headline:Fungal and Bacterial Killing by Neutrophils
      pageEnd:312
      pageStart:293
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-59745-204-5.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Host-Pathogen Interactions
         isbn:
            978-1-59745-204-5
            978-1-58829-886-7
         type:Book
      publisher:
         name:Humana Press
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:David Ermert
            affiliation:
                  name:Max Planck Institute for Infection Biology
                  address:
                     name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Arturo Zychlinsky
            affiliation:
                  name:Max Planck Institute for Infection Biology
                  address:
                     name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Constantin Urban
            affiliation:
                  name:Max Planck Institute for Infection Biology
                  address:
                     name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Neutrophils, phagocytosis, neutrophil extracellular traps, killing, survival, Candida albicans , Shigella flexneri
      description:Neutrophils are professional phagocytes of the innate immune system that are essential to control bacterial and fungal infections. These cells engulf and kill invading microbes. Additionally, activated neutrophils are able to release neutrophil extracellular traps (NETs). These fibers consist of chromatin decorated with antimicrobial proteins to trap and kill microbes. Appropriate quantitative methods are required to understand the nature of interactions of neutrophils with pathogens. Here we present assays to measure killing mediated by phagocytosis, by NETs, by a combination of both, and by granular extract. As examples, we use Candida albicans for fungal and Shigella flexneri for bacterial pathogens.
      datePublished:2009
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Book:
      name:Host-Pathogen Interactions
      isbn:
         978-1-59745-204-5
         978-1-58829-886-7
Organization:
      name:Humana Press
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         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Max Planck Institute for Infection Biology
      address:
         name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
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      name:Max Planck Institute for Infection Biology
      address:
         name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
         type:PostalAddress
      name:Max Planck Institute for Infection Biology
      address:
         name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
         type:PostalAddress
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Person:
      name:David Ermert
      affiliation:
            name:Max Planck Institute for Infection Biology
            address:
               name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
               type:PostalAddress
            type:Organization
      name:Arturo Zychlinsky
      affiliation:
            name:Max Planck Institute for Infection Biology
            address:
               name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
               type:PostalAddress
            type:Organization
      name:Constantin Urban
      affiliation:
            name:Max Planck Institute for Infection Biology
            address:
               name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
      name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
      name:Department of Cellular Microbiology, Max Planck Institute for Infection Biology, Germany
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