We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-9036-8_2.

Title:
Isolation of Human Peripheral Blood T-Lymphocytes | SpringerLink
Description:
Peripheral blood is the most common source of T-lymphocytes for in vitro culture. Here, we present a simple and standardized method for small- or large-scale isolation of viable T-lymphocytes and other mononuclear cells from fresh peripheral blood or buffy coat blood...
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28 years and 1 months (reg. 1997-05-29).

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Keywords {🔍}

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Topics {✒️}

month download article/chapter human primary t-cells t-cell motility step hypaque-ficoll method t-cell migration human peripheral blood academic research fund fresh peripheral blood privacy choices/manage cookies t-cells obtained viable t-lymphocytes device instant download altered lymphocyte markers springer nature large-scale isolation european economic area density gradient centrifugation lfa-1/icam-1 ligation protein kinase cε post-translational modifications author information authors editor information editors seow theng ong journal finder publish conditions privacy policy gapmer cellular internalization t-lymphocytes accepting optional cookies human blood verma nk main content log protocol usd 49 blood samples fazil mhut protocol cite protocol kizhakeyil social media permissions reprints mononuclear cells check access ethics access human memory ong st chalasani mls atish kizhakeyil cell separations cell recovery cell functionality moe-acrf tier 2 humana press

Schema {🗺️}

ScholarlyArticle:
      headline:Isolation of Human Peripheral Blood T-Lymphocytes
      pageEnd:17
      pageStart:11
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-9036-8.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:T-Cell Motility
         isbn:
            978-1-4939-9036-8
            978-1-4939-9035-1
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Atish Kizhakeyil
            affiliation:
                  name:Nanyang Technological University Singapore
                  address:
                     name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Seow Theng Ong
            affiliation:
                  name:Nanyang Technological University Singapore
                  address:
                     name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mobashar Hussain Urf Turabe Fazil
            affiliation:
                  name:Nanyang Technological University Singapore
                  address:
                     name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Madhavi Latha Somaraju Chalasani
            affiliation:
                  name:Hospital for Special Surgery
                  address:
                     name:Autoimmunity and Inflammation Program, Hospital for Special Surgery, New York, USA
                     type:PostalAddress
                  type:Organization
                  name:Nanyang Technological University Singapore
                  address:
                     name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Praseetha Prasannan
            affiliation:
                  name:Nanyang Technological University Singapore
                  address:
                     name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Navin Kumar Verma
            affiliation:
                  name:Nanyang Technological University Singapore
                  address:
                     name:Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:T-Lymphocytes, PBMC, Density gradient, Ficoll
      description:Peripheral blood is the most common source of T-lymphocytes for in vitro culture. Here, we present a simple and standardized method for small- or large-scale isolation of viable T-lymphocytes and other mononuclear cells from fresh peripheral blood or buffy coat blood samples using the density gradient centrifugation. T-cells obtained using the protocol described here can be used for a variety of downstream analysis, including cellular, molecular, and functional assays.
      datePublished:2019
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Book:
      name:T-Cell Motility
      isbn:
         978-1-4939-9036-8
         978-1-4939-9035-1
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      name:Springer New York
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         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Nanyang Technological University Singapore
      address:
         name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
         type:PostalAddress
      name:Nanyang Technological University Singapore
      address:
         name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
         type:PostalAddress
      name:Nanyang Technological University Singapore
      address:
         name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
         type:PostalAddress
      name:Hospital for Special Surgery
      address:
         name:Autoimmunity and Inflammation Program, Hospital for Special Surgery, New York, USA
         type:PostalAddress
      name:Nanyang Technological University Singapore
      address:
         name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
         type:PostalAddress
      name:Nanyang Technological University Singapore
      address:
         name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
         type:PostalAddress
      name:Nanyang Technological University Singapore
      address:
         name:Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
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ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Atish Kizhakeyil
      affiliation:
            name:Nanyang Technological University Singapore
            address:
               name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Seow Theng Ong
      affiliation:
            name:Nanyang Technological University Singapore
            address:
               name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
               type:PostalAddress
            type:Organization
      name:Mobashar Hussain Urf Turabe Fazil
      affiliation:
            name:Nanyang Technological University Singapore
            address:
               name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
               type:PostalAddress
            type:Organization
      name:Madhavi Latha Somaraju Chalasani
      affiliation:
            name:Hospital for Special Surgery
            address:
               name:Autoimmunity and Inflammation Program, Hospital for Special Surgery, New York, USA
               type:PostalAddress
            type:Organization
            name:Nanyang Technological University Singapore
            address:
               name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
               type:PostalAddress
            type:Organization
      name:Praseetha Prasannan
      affiliation:
            name:Nanyang Technological University Singapore
            address:
               name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
               type:PostalAddress
            type:Organization
      name:Navin Kumar Verma
      affiliation:
            name:Nanyang Technological University Singapore
            address:
               name:Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
      name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
      name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
      name:Autoimmunity and Inflammation Program, Hospital for Special Surgery, New York, USA
      name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
      name:Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
      name:Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore
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