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Title:
Microglia Analysis in Retinal Degeneration Mouse Models | SpringerLink
Description:
Microgliosis is a hallmark of degenerative processes in the retina. Reactive microglia migrate to the photoreceptor layer and the subretinal space during outer retinal degeneration. This process creates a toxic milieu where reactive microglia and dying photoreceptors...
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Keywords {🔍}

retinal, mouse, microglia, article, degeneration, protocol, pubmed, google, scholar, privacy, cookies, content, information, publish, langmann, access, springer, analysis, search, models, retina, photoreceptor, chapter, scholz, download, usd, function, personal, data, log, journal, research, phenotyping, dannhausen, rashid, thomas, book, reactive, phagocytes, model, retinitis, pigmentosa, open, karlstetter, caramoy, mol, author, nature, ebook, optional,

Topics {✒️}

month download article/chapter mouse retinal phenotyping fam161a-deficient mouse model university hospital schleswig-holstein khalid rashid & thomas langmann privacy choices/manage cookies device instant download outer retinal degeneration inherited retinal degeneration progressive retinal degeneration editor information editors evaluating microglia reactivity living photoreceptors contributes reactive microglia migrate springer nature european economic area accelerate photoreceptor demise performing immunohistochemical stainings monocyte-derived macrophages targeting translocator protein interferon-beta signaling journal finder publish conditions privacy policy mouse retina alter accepting optional cookies retinal degeneration protocol dannhausen main content log retinal microglia protocol usd 49 author correspondence check access ethics access mouse models microglia analysis reactive microglia protocol cite mouse model social media retinal cryosections permissions reprints photoreceptor degeneration thomas langmann usage analysis comprehensive analysis humana press chapter log journal publish privacy policy microglia contributes

Questions {❓}

Karlstetter M, Scholz R, Rutar M et al (2015) Retinal microglia: just bystander or target for therapy?

Schema {🗺️}

ScholarlyArticle:
      headline:Microglia Analysis in Retinal Degeneration Mouse Models
      pageEnd:166
      pageStart:159
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-7720-8.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Mouse Retinal Phenotyping
         isbn:
            978-1-4939-7720-8
            978-1-4939-7719-2
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Katharina Dannhausen
            affiliation:
                  name:University of Cologne
                  address:
                     name:Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, Cologne, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Khalid Rashid
            affiliation:
                  name:University of Cologne
                  address:
                     name:Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, Cologne, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Thomas Langmann
            affiliation:
                  name:University of Cologne
                  address:
                     name:Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, Cologne, Germany
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Microglia, Retinal degeneration, Immunohistochemistry, Cryosections, Flat mounts, Iba1, Fam161a
      description:Microgliosis is a hallmark of degenerative processes in the retina. Reactive microglia migrate to the photoreceptor layer and the subretinal space during outer retinal degeneration. This process creates a toxic milieu where reactive microglia and dying photoreceptors recruit additional reactive phagocytes. This results in the release of a multitude of proinflammatory factors which accelerate photoreceptor demise. In this chapter, we outline in detail how to monitor microgliosis in the Fam161a-deficient mouse model of Retinitis Pigmentosa by performing immunohistochemical stainings of retinal cryosections and flat mounts using the marker Iba1. This protocol will serve as a guideline in evaluating microglia reactivity and localization in various mouse models of retinal degeneration.
      datePublished:2018
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Book:
      name:Mouse Retinal Phenotyping
      isbn:
         978-1-4939-7720-8
         978-1-4939-7719-2
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      name:Springer New York
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      name:University of Cologne
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         name:Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, Cologne, Germany
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         name:Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, Cologne, Germany
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            address:
               name:Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, Cologne, Germany
               type:PostalAddress
            type:Organization
      name:Khalid Rashid
      affiliation:
            name:University of Cologne
            address:
               name:Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, Cologne, Germany
               type:PostalAddress
            type:Organization
      name:Thomas Langmann
      affiliation:
            name:University of Cologne
            address:
               name:Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, Cologne, Germany
               type:PostalAddress
            type:Organization
      email:[email protected]
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      name:Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, Cologne, Germany
      name:Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, Cologne, Germany
      name:Laboratory for Experimental Immunology of the Eye, Department of Ophthalmology, University of Cologne, Cologne, Germany
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