We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-7204-3_13.

Title:
Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine | SpringerLink
Description:
Isolation of newly transcribed RNA is an invaluable approach that can be used to study the dynamic life of RNA in cellulo. Traditional methods of whole-cell RNA extraction limit subsequent gene expression analyses to the steady-state levels of RNA abundance, which...
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Keywords {🔍}

rna, pubmed, processing, labeling, google, scholar, article, central, newly, metabolic, cas, privacy, cookies, content, information, publish, protocol, transcribed, hertel, synthesis, chapter, research, search, isolation, thiouridine, garibaldi, carranza, molecular, biol, author, data, log, journal, mrna, angela, francisco, klemens, methods, gene, expression, access, decay, california, irvine, springer, optional, media, personal, parties, policy,

Topics {✒️}

nascent pre-mrna privacy choices/manage cookies steady-state levels genome-wide technology editor information editors metabolic label 4-thiouridine european economic area downstream applications aimed recent advantages based nucleolar stress response b233 med sci newly transcribed rna newly synthesized rna rna uncovers principles journal finder publish conditions privacy policy modified nucleotide labeling francisco carranza & klemens protocol garibaldi accepting optional cookies determining rna stability main content log beggs jd mrna processing social media permissions reprints protocol cite author correspondence cell culture vivo kinetics humana press journal publish privacy policy personal data check access ethics access books a rna synthesis metabolic labeling optional cookies manage preferences online chapter describes ny chapter log rna abundance rna biol 9 rna production rna biol 10 data protection

Schema {🗺️}

ScholarlyArticle:
      headline:Isolation of Newly Transcribed RNA Using the Metabolic Label 4-Thiouridine
      pageEnd:176
      pageStart:169
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-7204-3.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:mRNA Processing
         isbn:
            978-1-4939-7204-3
            978-1-4939-7203-6
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Angela Garibaldi
            affiliation:
                  name:University of California, Irvine
                  address:
                     name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Francisco Carranza
            affiliation:
                  name:University of California, Irvine
                  address:
                     name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Klemens J. Hertel
            affiliation:
                  name:University of California, Irvine
                  address:
                     name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:4sU, 4sU-seq, Mammalian cells, Nascent RNA, Decay, Transcription, Nascent pre-mRNA, mRNA processing, Metabolic labeling, 4-Thiouridine
      description:Isolation of newly transcribed RNA is an invaluable approach that can be used to study the dynamic life of RNA in cellulo. Traditional methods of whole-cell RNA extraction limit subsequent gene expression analyses to the steady-state levels of RNA abundance, which often masks changes in RNA synthesis and processing. This chapter describes a methodology with low cytotoxicity that permits the labeling and isolation of nascent pre-mRNA in cell culture. The resulting isolate is suitable for use in a series of downstream applications aimed at studying changes in RNA synthesis, processing, or stability.
      datePublished:2017
      isAccessibleForFree:
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Book:
      name:mRNA Processing
      isbn:
         978-1-4939-7204-3
         978-1-4939-7203-6
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of California, Irvine
      address:
         name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
         type:PostalAddress
      name:University of California, Irvine
      address:
         name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
         type:PostalAddress
      name:University of California, Irvine
      address:
         name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Angela Garibaldi
      affiliation:
            name:University of California, Irvine
            address:
               name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
               type:PostalAddress
            type:Organization
      name:Francisco Carranza
      affiliation:
            name:University of California, Irvine
            address:
               name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
               type:PostalAddress
            type:Organization
      name:Klemens J. Hertel
      affiliation:
            name:University of California, Irvine
            address:
               name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
      name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
      name:Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, USA
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