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LINK . SPRINGER . COM {}

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We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-6993-7_1.

Title:
Quantitation of Poly(ADP-Ribose) by Isotope Dilution Mass Spectrometry | SpringerLink
Description:
Poly(ADP-ribosyl)ation (PARylation), i.e., the formation of the nucleic acid-like biopolymer poly(ADP-ribose) (PAR), is an essential posttranslational modification carried out by poly(ADP-ribose) polymerases (PARPs). While PAR levels are low under physiological...
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

  • Science
  • Education
  • Telecommunications

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 7,734,772 visitors per month in the current month.

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How Does Link.springer.com Make Money? {💸}

We don’t know how the website earns money.

Many websites are intended to earn money, but some serve to share ideas or build connections. Websites exist for all kinds of purposes. This might be one of them. Link.springer.com could be getting rich in stealth mode, or the way it's monetizing isn't detectable.

Keywords {🔍}

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Topics {✒️}

month download article/chapter liquid‒liquid phase separation uplc-ms/ms instrumentation ultraviolet radiation-induced stress privacy choices/manage cookies collaborative research center infection-induced colitis device instant download unequivocal chemical specificity american chemical society lc-ms/ms stress-induced levels steady-state levels pubmed pmid bioanalytical platform based author information authors editor information editors european economic area carcinogen-treated cells detect parp-1 activation l-selegiline potentiates german federal ministry mass spectrometry journal finder publish primary human lymphocytes dna damage leading conditions privacy policy adenosine diphosphate ribose accepting optional cookies polymerase tabea zubel specific binding proteins distinctive cellular consequences main content log aswin mangerich shah rg quantify cellular par social media tissue-specific permissions reprints basic research protein research stress response protocol usd 49 absolute terms check access biological consequences humana press ethics access molecular biology journal publish

Schema {🗺️}

ScholarlyArticle:
      headline:Quantitation of Poly(ADP-Ribose) by Isotope Dilution Mass Spectrometry
      pageEnd:18
      pageStart:3
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-6993-7.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Poly(ADP-Ribose) Polymerase
         isbn:
            978-1-4939-6993-7
            978-1-4939-6992-0
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Tabea Zubel
            affiliation:
                  name:University of Konstanz
                  address:
                     name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Rita Martello
            affiliation:
                  name:University of Copenhagen
                  address:
                     name:Department of Proteomics, Faculty of Health and Medical Sciences, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Alexander Bürkle
            affiliation:
                  name:University of Konstanz
                  address:
                     name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Aswin Mangerich
            affiliation:
                  name:University of Konstanz
                  address:
                     name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Poly(ADP-ribose), PARP, ARTD, Mass spectrometry, UPLC, HPLC
      description:Poly(ADP-ribosyl)ation (PARylation), i.e., the formation of the nucleic acid-like biopolymer poly(ADP-ribose) (PAR), is an essential posttranslational modification carried out by poly(ADP-ribose) polymerases (PARPs). While PAR levels are low under physiological conditions, they can transiently increase more than 100-fold upon induction of genotoxic stress. The accurate quantitation of cellular PAR with high sensitivity is of critical importance to understand the role of PARylation in cellular physiology and pathophysiology and to determine the pharmacodynamic efficiencies of clinically relevant PARP inhibitors, which represent a novel class of promising chemotherapeutics. Previously, we have developed a bioanalytical platform based on isotope dilution mass spectrometry (LC-MS/MS) to quantify cellular PAR with unequivocal chemical specificity in absolute terms with femtomol sensitivity (Martello et al. ACS Chem Biol 8(7):1567–1575, 2013). This method enables the analysis of steady-state levels, as well as stress-induced levels of PAR in various biological systems including cell lines, mouse tissues, and primary human lymphocytes. It has a wide range of potential applications in basic research, as well as in drug development (Martello et al. ACS Chem Biol 8(7):1567–1575, 2013; Mangerich et al. Toxicol Lett 244:56–71, 2016). Here, we present an improved and adjusted version of the original protocol by Martello/Mangerich et al., which uses UPLC-MS/MS instrumentation.
      datePublished:2017
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:Poly(ADP-Ribose) Polymerase
      isbn:
         978-1-4939-6993-7
         978-1-4939-6992-0
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Konstanz
      address:
         name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
         type:PostalAddress
      name:University of Copenhagen
      address:
         name:Department of Proteomics, Faculty of Health and Medical Sciences, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark
         type:PostalAddress
      name:University of Konstanz
      address:
         name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
         type:PostalAddress
      name:University of Konstanz
      address:
         name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Tabea Zubel
      affiliation:
            name:University of Konstanz
            address:
               name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
               type:PostalAddress
            type:Organization
      name:Rita Martello
      affiliation:
            name:University of Copenhagen
            address:
               name:Department of Proteomics, Faculty of Health and Medical Sciences, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark
               type:PostalAddress
            type:Organization
      name:Alexander Bürkle
      affiliation:
            name:University of Konstanz
            address:
               name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
               type:PostalAddress
            type:Organization
      name:Aswin Mangerich
      affiliation:
            name:University of Konstanz
            address:
               name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
      name:Department of Proteomics, Faculty of Health and Medical Sciences, Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen, Denmark
      name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
      name:Molecular Toxicology Group, Department of Biology, University of Konstanz, Konstanz, Germany
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

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