We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-6481-9_11.

Title:
Adjuvant-Enhanced mRNA Vaccines | SpringerLink
Description:
Recent advances in molecular biology have led to dramatic enhancement of the stability of in vitro transcribed (IVT) messenger RNA (mRNA) and improvement in its translational efficacy. Nowadays, mRNA-based vaccines represent a promising approach in the field of...
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Keywords {🔍}

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Topics {✒️}

adjuvant-enhanced mrna vaccines mrna-based vaccines represent month download article/chapter cell-based vaccination approaches broad t-cell response single-step antigen loading long-term clinical outcome cross-presenting dendritic cells karine breckpot & kris thielemans van nuffel amt rna-based treatments trimix mrna results privacy choices/manage cookies mrna vaccine therapy rna vaccines alexia van weijnen van lint device instant download t-cell activation antigen presentation enhances national cancer plan stichting tegen kanker cd40l strongly enhances dynamic bioluminescence imaging melanoma patients treated european economic area messenger rna earlier-established bacteria experimental procedures employed pd-l1/pd-1 durable tumor response phase ib study direct intralymphatic injection hercules foundation flanders senior clinical investigator author information authors vrije universiteit brussel editor information editors protocol usd 49 effective immune responses ivt mrna encoding journal finder publish conditions privacy policy immune activation signals murine tumor models stimulate hiv-specific protocol bialkowski protocol cite accepting optional cookies cell responses

Schema {🗺️}

ScholarlyArticle:
      headline:Adjuvant-Enhanced mRNA Vaccines
      pageEnd:191
      pageStart:179
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-6481-9.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:RNA Vaccines
         isbn:
            978-1-4939-6481-9
            978-1-4939-6479-6
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Lukasz Bialkowski
            affiliation:
                  name:Vrije Universiteit Brussel
                  address:
                     name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Kevin Van der Jeught
            affiliation:
                  name:Vrije Universiteit Brussel
                  address:
                     name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
                     type:PostalAddress
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            type:Person
            name:Dries Renmans
            affiliation:
                  name:Vrije Universiteit Brussel
                  address:
                     name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Alexia van Weijnen
            affiliation:
                  name:Vrije Universiteit Brussel
                  address:
                     name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Carlo Heirman
            affiliation:
                  name:Vrije Universiteit Brussel
                  address:
                     name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Marleen Keyaerts
            affiliation:
                  name:Vrije Universiteit Brussel
                  address:
                     name:In Vivo Cellular and Molecular Imaging Laboratory, Vrije Universiteit Brussel, Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Karine Breckpot
            affiliation:
                  name:Vrije Universiteit Brussel
                  address:
                     name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Kris Thielemans
            affiliation:
                  name:Vrije Universiteit Brussel
                  address:
                     name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:mRNA, Electroporation, Intranodal, Intralymphatic, Intratumoral, Bioluminescence imaging, Therapeutic vaccine, Cancer immunotherapy, Dendritic cells
      description:Recent advances in molecular biology have led to dramatic enhancement of the stability of in vitro transcribed (IVT) messenger RNA (mRNA) and improvement in its translational efficacy. Nowadays, mRNA-based vaccines represent a promising approach in the field of anticancer immunotherapy, gaining attention over the earlier-established bacteria-, virus-, or cell-based vaccination approaches. Here, we present the experimental procedures employed in our laboratory to induce anticancer immune responses in different murine tumor models using IVT mRNA encoding for immune activation signals and antigens of interest.
      datePublished:2017
      isAccessibleForFree:
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Book:
      name:RNA Vaccines
      isbn:
         978-1-4939-6481-9
         978-1-4939-6479-6
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Vrije Universiteit Brussel
      address:
         name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel
      address:
         name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel
      address:
         name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel
      address:
         name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel
      address:
         name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
         type:PostalAddress
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      address:
         name:In Vivo Cellular and Molecular Imaging Laboratory, Vrije Universiteit Brussel, Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel
      address:
         name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel
      address:
         name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Lukasz Bialkowski
      affiliation:
            name:Vrije Universiteit Brussel
            address:
               name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Kevin Van der Jeught
      affiliation:
            name:Vrije Universiteit Brussel
            address:
               name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Dries Renmans
      affiliation:
            name:Vrije Universiteit Brussel
            address:
               name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Alexia van Weijnen
      affiliation:
            name:Vrije Universiteit Brussel
            address:
               name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Carlo Heirman
      affiliation:
            name:Vrije Universiteit Brussel
            address:
               name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Marleen Keyaerts
      affiliation:
            name:Vrije Universiteit Brussel
            address:
               name:In Vivo Cellular and Molecular Imaging Laboratory, Vrije Universiteit Brussel, Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Karine Breckpot
      affiliation:
            name:Vrije Universiteit Brussel
            address:
               name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Kris Thielemans
      affiliation:
            name:Vrije Universiteit Brussel
            address:
               name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
      name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
      name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
      name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
      name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
      name:In Vivo Cellular and Molecular Imaging Laboratory, Vrije Universiteit Brussel, Brussels, Belgium
      name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
      name:Laboratory of Molecular and Cellular Therapy, Vrije Universiteit Brussel, Brussels, Belgium
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