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  6. Keywords
  7. Topics
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We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-3810-0_12.

Title:
Flow Cytometry and Transplantation-Based Quantitative Assays for Satellite Cell Self-Renewal and Differentiation | SpringerLink
Description:
In response to muscle damage, satellite cells proliferate and undertake both differentiation and self-renewal, generating new functional muscle tissue and repopulating this new muscle with stem cells for future injury responses. For many questions relating to the...
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {๐Ÿ“š}

  • Telecommunications
  • Science
  • Education

Content Management System {๐Ÿ“}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {๐Ÿ“ˆ}

What is the average monthly size of link.springer.com audience?

๐ŸŒ  Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
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How Does Link.springer.com Make Money? {๐Ÿ’ธ}

We donโ€™t know how the website earns money.

Not all websites focus on profit; some are designed to educate, connect people, or share useful tools. People create websites for numerous reasons. And this could be one such example. Link.springer.com might have a hidden revenue stream, but it's not something we can detect.

Keywords {๐Ÿ”}

pubmed, article, cas, google, scholar, cell, muscle, satellite, cells, mouse, selfrenewal, differentiation, skeletal, protocol, stem, kyba, chapter, central, privacy, cookies, content, information, publish, muscular, dystrophy, springer, research, search, regeneration, quantitative, arpke, michael, molecular, nature, download, usd, personal, data, log, journal, phd, book, methods, functional, assay, transplantation, state, access, isolation, myogenic,

Topics {โœ’๏ธ}

month download article/chapter arpkeย &ย michael kyba ph myod-deficient myogenic cell chromosome-linked muscular dystrophy transplantation-based quantitative assays immuno- dystrophin-deficient model hoffman ep skeletal muscle regeneration privacy choices/manage cookies device instant download michael kyba ph skeletal myogenic progenitors adult skeletal muscle duchenne muscular dystrophy assay involving transplantation satellite cell state muscular dystrophy association donor cell identity european economic area future injury responses myogenic satellite cells immunoelectron microscopic localization defective lymphoid development watkins sc somatic reversion/suppression mesp1 patterns mesoderm context-dependent manner interferon regulatory factor-2 author information authors editor information editors journal finder publish conditions privacy policy satellite cells proliferate resident satellite cells murine satellite cells nsg-mdx mouse mouse mdx phenotype quiescent satellite cells functional muscle tissue brown rh jr dystrophin-glycoprotein complex accepting optional cookies pax7-zsgreen transgene provide detailed methods mice lacking expression main content log bosma mj protocol arpke quantitative assay social media

Schema {๐Ÿ—บ๏ธ}

ScholarlyArticle:
      headline:Flow Cytometry and Transplantation-Based Quantitative Assays for Satellite Cell Self-Renewal and Differentiation
      pageEnd:179
      pageStart:163
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3810-0.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Skeletal Muscle Regeneration in the Mouse
         isbn:
            978-1-4939-3810-0
            978-1-4939-3808-7
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Robert W. Arpke
            affiliation:
                  name:University of Minnesota
                  address:
                     name:Lillehei Heart Institute and Department of Pediatrics, University of Minnesota, Minneapolis, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Michael Kyba
            affiliation:
                  name:University of Minnesota
                  address:
                     name:Lillehei Heart Institute and Department of Pediatrics, University of Minnesota, Minneapolis, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Satellite cells, Pax7, Transplantation, Myogenesis
      description:In response to muscle damage, satellite cells proliferate and undertake both differentiation and self-renewal, generating new functional muscle tissue and repopulating this new muscle with stem cells for future injury responses. For many questions relating to the physiological regulation of satellite cells, quantitative readouts of self-renewal and differentiation can be very useful. There is a particular need for a quantitative assay for satellite cell self-renewal that does not rely solely upon sectioning, staining and counting cells in sections. In this chapter, we provide detailed methods for quantifying the self-renewal and differentiation potential of a given population of satellite cells using an assay involving transplantation into injured, regenerating muscle together with specific markers for donor cell identity and state of differentiation. In particular, using the Pax7-ZsGreen transgene as a marker of satellite cell state, self-renewal can be quantified by FACS on transplanted muscle to actually count the total number of resident satellite cells at time points following transplantation.
      datePublished:2016
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
      context:https://schema.org
Book:
      name:Skeletal Muscle Regeneration in the Mouse
      isbn:
         978-1-4939-3810-0
         978-1-4939-3808-7
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Minnesota
      address:
         name:Lillehei Heart Institute and Department of Pediatrics, University of Minnesota, Minneapolis, USA
         type:PostalAddress
      name:University of Minnesota
      address:
         name:Lillehei Heart Institute and Department of Pediatrics, University of Minnesota, Minneapolis, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Robert W. Arpke
      affiliation:
            name:University of Minnesota
            address:
               name:Lillehei Heart Institute and Department of Pediatrics, University of Minnesota, Minneapolis, USA
               type:PostalAddress
            type:Organization
      name:Michael Kyba
      affiliation:
            name:University of Minnesota
            address:
               name:Lillehei Heart Institute and Department of Pediatrics, University of Minnesota, Minneapolis, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Lillehei Heart Institute and Department of Pediatrics, University of Minnesota, Minneapolis, USA
      name:Lillehei Heart Institute and Department of Pediatrics, University of Minnesota, Minneapolis, USA
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {๐Ÿ”—}(95)

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