We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-3756-1_15.

Title:
Detection and Analysis of Cell Cycle-Associated APC/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo | SpringerLink
Description:
The anaphase-promoting complex or cyclosome (APC/C) is one of the major orchestrators of the cell division cycle in mammalian cells. The APC/C acts as a ubiquitin ligase that triggers sequential ubiquitylation of a significant number of substrates which will be...
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Topics {✒️}

measuring apc/c-dependent ubiquitylation apc/c-mediated cellular ubiquitylation month download article/chapter anaphase promoting complex/cyclosome anaphase-promoting complex induces k11 linkage-specific antibody human anaphase-promoting complex brown ng device instant download vub research council privacy choices/manage cookies ubiquitin chain initiation ubiquitin chain assembly anaphase-promoting complex anaphase promoting complex triggers sequential ubiquitylation e3 ligase shutdown jnk-mediated phosphorylation protein stability provoked branched ubiquitin chains esther la monaca dna damage checkpoint human cytomegalovirus ul21a unique cellular mechanism editor information editors acknowledge networking support gutierrez gj ring e3 mechanism enhanced protein degradation present accessible methodologies crucial role played controlling chromosome division van voorhis va multiple mechanisms determine single-molecule study da fonseca pc vrije universiteit brussel cell cycle control pathophysiological cell signaling proteostasis cost action cell division cycle ncb2071 [pii] 10 frye jj iap-vii/07 program seventh framework program european economic area actively proliferating cells emerging regulatory mechanisms k11-linked polyubiquitination journal finder publish

Schema {🗺️}

ScholarlyArticle:
      headline:Detection and Analysis of Cell Cycle-Associated APC/C-Mediated Cellular Ubiquitylation In Vitro and In Vivo
      pageEnd:265
      pageStart:251
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3756-1.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Proteostasis
         isbn:
            978-1-4939-3756-1
            978-1-4939-3754-7
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Cesyen Cedeño
            affiliation:
                  name:Vrije Universiteit Brussel (VUB)
                  address:
                     name:Department of Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), Vrije Universiteit Brussel (VUB), Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Esther La Monaca
            affiliation:
                  name:Vrije Universiteit Brussel (VUB)
                  address:
                     name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Mara Esposito
            affiliation:
                  name:Vrije Universiteit Brussel (VUB)
                  address:
                     name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Gustavo J. Gutierrez
            affiliation:
                  name:Vrije Universiteit Brussel (VUB)
                  address:
                     name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Anaphase-promoting complex or cyclosome (APC/C), Cell cycle, Ubiquitin, Ubiquitylation, Phosphorylation, Cdc27/APC3, Cdh1, Cdc20
      description:The anaphase-promoting complex or cyclosome (APC/C) is one of the major orchestrators of the cell division cycle in mammalian cells. The APC/C acts as a ubiquitin ligase that triggers sequential ubiquitylation of a significant number of substrates which will be eventually degraded by proteasomes during major transitions of the cell cycle. In this chapter, we present accessible methodologies to assess both in in vitro conditions and in cellular systems ubiquitylation reactions mediated by the APC/C. In addition, we also describe techniques to evidence the changes in protein stability provoked by modulation of the activity of the APC/C. Finally, specific methods to analyze interactors or posttranslational modifications of particular APC/C subunits are also discussed. Given the crucial role played by the APC/C in the regulation of the cell cycle, this review only focuses on its action and effects in actively proliferating cells.
      datePublished:2016
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Book:
      name:Proteostasis
      isbn:
         978-1-4939-3756-1
         978-1-4939-3754-7
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      name:Springer New York
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         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Vrije Universiteit Brussel (VUB)
      address:
         name:Department of Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), Vrije Universiteit Brussel (VUB), Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel (VUB)
      address:
         name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel (VUB)
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         name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
         type:PostalAddress
      name:Vrije Universiteit Brussel (VUB)
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         name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
         type:PostalAddress
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Person:
      name:Cesyen Cedeño
      affiliation:
            name:Vrije Universiteit Brussel (VUB)
            address:
               name:Department of Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), Vrije Universiteit Brussel (VUB), Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Esther La Monaca
      affiliation:
            name:Vrije Universiteit Brussel (VUB)
            address:
               name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Mara Esposito
      affiliation:
            name:Vrije Universiteit Brussel (VUB)
            address:
               name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
               type:PostalAddress
            type:Organization
      name:Gustavo J. Gutierrez
      affiliation:
            name:Vrije Universiteit Brussel (VUB)
            address:
               name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Structural Biology, Vlaams Instituut voor Biotechnologie (VIB), Vrije Universiteit Brussel (VUB), Brussels, Belgium
      name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
      name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
      name:Laboratory of Pathophysiological Cell Signaling (PACS), Department of Biology, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel (VUB), Brussels, Belgium
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