We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-3603-8_4.

Title:
An Air–Liquid Interface Culture System for 3D Organoid Culture of Diverse Primary Gastrointestinal Tissues | SpringerLink
Description:
Conventional in vitro analysis of gastrointestinal epithelium usually relies on two-dimensional (2D) culture of epithelial cell lines as monolayer on impermeable surfaces. However, the lack of context of differentiation and tissue architecture in 2D culture can...
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Keywords {🔍}

pubmed, culture, article, cas, google, scholar, cell, gastrointestinal, stem, protocol, primary, epithelial, intestinal, cells, ootani, privacy, cookies, content, information, publish, chapter, central, search, system, organoid, tissues, kuo, molecular, vitro, epithelium, longterm, organoids, download, springer, usd, personal, data, log, journal, research, physiology, diseases, airliquid, interface, diverse, calvin, book, methods, threedimensional, niche,

Topics {✒️}

month download article/chapter form endocrine/acinar colonies lgr5/r-spondin axis akifumi ootani & calvin kuo primary intestinal organoids diverse gastrointestinal tissues primary organoid culture privacy choices/manage cookies epithelial cell lines 3d organoid culture intestinal stem cells adult mouse pancreas long-term culture stem cell niche protocol gastrointestinal physiology robust long-term device instant download primary gastrointestinal epithelium colony-forming cells stem cells drive dimensional primary culture european economic area mesenchymal/stromal components microenvironment accurately recapitulating van es jh organizing mini-guts promote contractile behavior nih grants u19ai116484 author information authors editor information editors journal finder publish conditions privacy policy marker gene lgr5 accepting optional cookies diseases xingnan li stanford university school main content log adult mouse gastrointestinal physiology long-term gastrointestinal culture stem cells calvin kuo social media permissions reprints intestinal epithelium protocol usd 49 chapter log protocol li humana press

Schema {🗺️}

ScholarlyArticle:
      headline:An Air–Liquid Interface Culture System for 3D Organoid Culture of Diverse Primary Gastrointestinal Tissues
      pageEnd:40
      pageStart:33
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3603-8.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Gastrointestinal Physiology and Diseases
         isbn:
            978-1-4939-3603-8
            978-1-4939-3601-4
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Xingnan Li
            affiliation:
                  name:Stanford University School of Medicine
                  address:
                     name:Hematology Division, Department of Medicine, Stanford University School of Medicine, Stanford, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Akifumi Ootani
            affiliation:
                  name:Stanford University School of Medicine
                  address:
                     name:Hematology Division, Department of Medicine, Stanford University School of Medicine, Stanford, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Calvin Kuo
            affiliation:
                  name:Stanford University School of Medicine
                  address:
                     name:Hematology Division, Department of Medicine, Stanford University School of Medicine, Stanford, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:Gastrointestinal tissue culture, Organoid, Three dimensional culture, Air–liquid interface, Stem cell niche
      description:Conventional in vitro analysis of gastrointestinal epithelium usually relies on two-dimensional (2D) culture of epithelial cell lines as monolayer on impermeable surfaces. However, the lack of context of differentiation and tissue architecture in 2D culture can hinder the faithful recapitulation of the phenotypic and morphological characteristics of native epithelium. Here, we describe a robust long-term three-dimensional (3D) culture methodology for gastrointestinal culture, which incorporates both epithelial and mesenchymal/stromal components into a collagen-based air–liquid interface 3D culture system. This system allows vigorously expansion of primary gastrointestinal epithelium for over 60 days as organoids with both proliferation and multilineage differentiation, indicating successful long-term intestinal culture within a microenvironment accurately recapitulating the stem cell niche.
      datePublished:2016
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Book:
      name:Gastrointestinal Physiology and Diseases
      isbn:
         978-1-4939-3603-8
         978-1-4939-3601-4
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Stanford University School of Medicine
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         name:Hematology Division, Department of Medicine, Stanford University School of Medicine, Stanford, USA
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      name:Stanford University School of Medicine
      address:
         name:Hematology Division, Department of Medicine, Stanford University School of Medicine, Stanford, USA
         type:PostalAddress
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Person:
      name:Xingnan Li
      affiliation:
            name:Stanford University School of Medicine
            address:
               name:Hematology Division, Department of Medicine, Stanford University School of Medicine, Stanford, USA
               type:PostalAddress
            type:Organization
      name:Akifumi Ootani
      affiliation:
            name:Stanford University School of Medicine
            address:
               name:Hematology Division, Department of Medicine, Stanford University School of Medicine, Stanford, USA
               type:PostalAddress
            type:Organization
      name:Calvin Kuo
      affiliation:
            name:Stanford University School of Medicine
            address:
               name:Hematology Division, Department of Medicine, Stanford University School of Medicine, Stanford, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Hematology Division, Department of Medicine, Stanford University School of Medicine, Stanford, USA
      name:Hematology Division, Department of Medicine, Stanford University School of Medicine, Stanford, USA
      name:Hematology Division, Department of Medicine, Stanford University School of Medicine, Stanford, USA
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