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Assessing Extracellular ATP as Danger Signal In Vivo: The pmeLuc System | SpringerLink
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Inflammation is the key pathophysiological response triggered by noxious agents in multicellular organisms. Central to inflammation is detection of exogenous or endogenous danger signals by immune cells. Extracellular ATP is a ubiquitous danger signal released during...
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Keywords {🔍}
pubmed, article, atp, virgilio, google, scholar, extracellular, vivo, central, cas, protocol, privacy, cookies, content, information, publish, pinton, falzoni, inflammation, access, cell, research, search, danger, francesco, download, ferrara, springer, ebook, personal, data, log, journal, nlr, proteins, signal, book, molecular, plasma, bioluminescence, chapter, open, pellegatti, author, york, usd, optional, media, parties, policy,
Topics {✒️}
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ScholarlyArticle:
headline:Assessing Extracellular ATP as Danger Signal In Vivo: The pmeLuc System
pageEnd:129
pageStart:115
image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3566-6.jpg
genre:
Springer Protocols
isPartOf:
name:NLR Proteins
isbn:
978-1-4939-3566-6
978-1-4939-3564-2
type:Book
publisher:
name:Springer New York
logo:
url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
type:ImageObject
type:Organization
author:
name:Francesco Di Virgilio
affiliation:
name:University of Ferrara
address:
name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
type:PostalAddress
type:Organization
email:[email protected]
type:Person
name:Paolo Pinton
affiliation:
name:University of Ferrara
address:
name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
type:PostalAddress
type:Organization
type:Person
name:Simonetta Falzoni
affiliation:
name:University of Ferrara
address:
name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
type:PostalAddress
type:Organization
type:Person
keywords:Extracellular ATP, Plasma membrane luciferase, Luciferin, Luminescence, Luminometry
description:Inflammation is the key pathophysiological response triggered by noxious agents in multicellular organisms. Central to inflammation is detection of exogenous or endogenous danger signals by immune cells. Extracellular ATP is a ubiquitous danger signal released during septic or sterile inflammation. The development of reliable techniques to measure extracellular ATP in vivo has become an urgent need in inflammation studies after the discovery that the most potent plasma membrane receptor responsible for NLRP3 inflammasome activation is an ATP-activated receptor, P2RX7. Here we describe an easy bioluminescence technique for the measurement of extracellular ATP in vivo.
datePublished:2016
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isbn:
978-1-4939-3566-6
978-1-4939-3564-2
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name:Springer New York
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name:University of Ferrara
address:
name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
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name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
type:PostalAddress
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name:Francesco Di Virgilio
affiliation:
name:University of Ferrara
address:
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name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
name:Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy
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