We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-3112-5_10.

Title:
Delivery of Small Interfering RNAs to Cells via Exosomes | SpringerLink
Description:
Exosomes are small membrane bound vesicles between 30 and 100 nm in diameter of endocytic origin that are secreted into the extracellular environment by many different cell types. Exosomes play a role in intercellular communication by transferring proteins, lipids,...
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Keywords {🔍}

pubmed, exosomes, article, google, scholar, cas, cells, human, delivery, protocol, telemo, valadi, research, sirna, methods, rnas, vesicles, central, privacy, cookies, content, information, publish, small, wahlgren, cell, search, interfering, skogberg, chapter, raposo, immunol, download, springer, usd, analysis, personal, data, log, journal, statello, hadi, book, molecular, extracellular, access, author, usa, york, ebook,

Topics {✒️}

month download article/chapter antigen-presenting cell vesicles tcr/cd3/zeta complex immunogenic tumour-derived exosomes dendritic cell-derived exosomes sirna delivery methods esbjörn telemo & hadi valadi van der post privacy choices/manage cookies sirna inside exosomes device instant download editor information editors small interfering rnas melanoma-derived exosomes mast cell-dependent functional small rnas european economic area exosome-mediated transfer immunologically active exosomes protocol wahlgren cells secrete exosomes journal finder publish conditions privacy policy extracellular vesicle sizing nanoparticle tracking analysis human blood plasma human bal fluid human thymic exosomes accepting optional cookies intestinal epithelial cells main content log protocol usd 49 wood mj inflammation research protocol cite author correspondence chapter log hadi valadi social media extracellular vesicles permissions reprints cell biol 147 journal publish privacy policy check access ethics access sargent il books a plasma exosomes sirna

Schema {🗺️}

ScholarlyArticle:
      headline:Delivery of Small Interfering RNAs to Cells via Exosomes
      pageEnd:125
      pageStart:105
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3112-5.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:SiRNA Delivery Methods
         isbn:
            978-1-4939-3112-5
            978-1-4939-3111-8
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Jessica Wahlgren
            affiliation:
                  name:University of Gothenburg
                  address:
                     name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Luisa Statello
            affiliation:
                  name:University of Gothenburg
                  address:
                     name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Gabriel Skogberg
            affiliation:
                  name:University of Gothenburg
                  address:
                     name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Esbjörn Telemo
            affiliation:
                  name:University of Gothenburg
                  address:
                     name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Hadi Valadi
            affiliation:
                  name:University of Gothenburg
                  address:
                     name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords: Exosomes , Extracellular vesicles, Electroporation , siRNA delivery, Plasma exosomes, Exosome delivery, Exosome uptake, Post-transcriptional gene silencing
      description:Exosomes are small membrane bound vesicles between 30 and 100 nm in diameter of endocytic origin that are secreted into the extracellular environment by many different cell types. Exosomes play a role in intercellular communication by transferring proteins, lipids, and RNAs to recipient cells. Exosomes from human cells could be used as vectors to provide cells with therapeutic RNAs. Here we describe how exogenous small interfering RNAs may successfully be introduced into various kinds of human exosomes using electroporation and subsequently delivered to recipient cells. Methods used to confirm the presence of siRNA inside exosomes and cells are presented, such as flow cytometry, confocal microscopy, and Northern blot.
      datePublished:2016
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Book:
      name:SiRNA Delivery Methods
      isbn:
         978-1-4939-3112-5
         978-1-4939-3111-8
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Gothenburg
      address:
         name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
         type:PostalAddress
      name:University of Gothenburg
      address:
         name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
         type:PostalAddress
      name:University of Gothenburg
      address:
         name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
         type:PostalAddress
      name:University of Gothenburg
      address:
         name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
         type:PostalAddress
      name:University of Gothenburg
      address:
         name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Jessica Wahlgren
      affiliation:
            name:University of Gothenburg
            address:
               name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
               type:PostalAddress
            type:Organization
      name:Luisa Statello
      affiliation:
            name:University of Gothenburg
            address:
               name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
               type:PostalAddress
            type:Organization
      name:Gabriel Skogberg
      affiliation:
            name:University of Gothenburg
            address:
               name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
               type:PostalAddress
            type:Organization
      name:Esbjörn Telemo
      affiliation:
            name:University of Gothenburg
            address:
               name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
               type:PostalAddress
            type:Organization
      name:Hadi Valadi
      affiliation:
            name:University of Gothenburg
            address:
               name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
      name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
      name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
      name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
      name:Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
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