We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-3067-8_13.

Title:
Capture and Identification of miRNA Targets by Biotin Pulldown and RNA-seq | SpringerLink
Description:
MicroRNAs (miRNAs) are small noncoding RNAs that regulate the stability and expression of target RNAs in a sequence-dependent manner. Identifying miRNA-regulated genes is key to understanding miRNA function. Here, we describe an unbiased biochemical pulldown method...
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Keywords {🔍}

pubmed, article, google, scholar, cas, central, cell, mirna, lieberman, microrna, protocol, target, identification, tan, privacy, cookies, content, information, publish, gene, targets, mrnas, chapter, binding, mol, biol, research, search, regulation, judy, micrornas, sites, lal, nat, argonaute, nature, protein, download, springer, usd, function, personal, data, log, journal, posttranscriptional, capture, pulldown, shen, mynn,

Topics {✒️}

post-transcriptional gene regulation month download article/chapter tumor suppressor mir-34a microrna-bound mrnas identifies transcriptome-wide identification high-specificity mirna targets identifying mirna-regulated genes microrna binding sites martin hc rna-binding protein microrna target sites protocol tan privacy choices/manage cookies tan sm device instant download microrna-10a binds microrna-125b occurs judy lieberman le mt mir-200 promotes ribosomal protein mrnas 3′-biotin-tagged microrna-27 conserved regulation european economic area sequence-dependent manner involve cross-linking bartel dp human mirna interactome growth factor signaling davis-dusenbery bn p53 network dosage author information authors harvard medical school editor information editors understanding mirna function molecular medicine program protocol cite journal finder publish conditions privacy policy pattern-based method target site interactions streptavidin-captured mrnas protocol usd 49 small noncoding rnas conserved seed pairing isomirs function cooperatively accepting optional cookies suppressing multiple members main content log regulated transcripts

Schema {🗺️}

ScholarlyArticle:
      headline:Capture and Identification of miRNA Targets by Biotin Pulldown and RNA-seq
      pageEnd:228
      pageStart:211
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-3067-8.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Post-Transcriptional Gene Regulation
         isbn:
            978-1-4939-3067-8
            978-1-4939-3066-1
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Shen Mynn Tan
            affiliation:
                  name:Boston Children’s Hospital
                  address:
                     name:Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, USA
                     type:PostalAddress
                  type:Organization
                  name:Harvard Medical School
                  address:
                     name:Department of Pediatrics, Harvard Medical School, Boston, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Judy Lieberman
            affiliation:
                  name:Boston Children’s Hospital
                  address:
                     name:Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, USA
                     type:PostalAddress
                  type:Organization
                  name:Harvard Medical School
                  address:
                     name:Department of Pediatrics, Harvard Medical School, Boston, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:MicroRNA, Noncoding RNA , miRNA recognition element, Target identification
      description:MicroRNAs (miRNAs) are small noncoding RNAs that regulate the stability and expression of target RNAs in a sequence-dependent manner. Identifying miRNA-regulated genes is key to understanding miRNA function. Here, we describe an unbiased biochemical pulldown method to identify with high-specificity miRNA targets. Regulated transcripts are enriched in streptavidin-captured mRNAs that bind to a transfected biotinylated miRNA mimic. The method is relatively simple, does not involve cross-linking and can be performed with only a million cells. Addition of an on-bead RNase digestion step also identifies miRNA recognition elements (MRE).
      datePublished:2016
      isAccessibleForFree:
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      context:https://schema.org
Book:
      name:Post-Transcriptional Gene Regulation
      isbn:
         978-1-4939-3067-8
         978-1-4939-3066-1
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Boston Children’s Hospital
      address:
         name:Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, USA
         type:PostalAddress
      name:Harvard Medical School
      address:
         name:Department of Pediatrics, Harvard Medical School, Boston, USA
         type:PostalAddress
      name:Boston Children’s Hospital
      address:
         name:Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, USA
         type:PostalAddress
      name:Harvard Medical School
      address:
         name:Department of Pediatrics, Harvard Medical School, Boston, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Shen Mynn Tan
      affiliation:
            name:Boston Children’s Hospital
            address:
               name:Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, USA
               type:PostalAddress
            type:Organization
            name:Harvard Medical School
            address:
               name:Department of Pediatrics, Harvard Medical School, Boston, USA
               type:PostalAddress
            type:Organization
      name:Judy Lieberman
      affiliation:
            name:Boston Children’s Hospital
            address:
               name:Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, USA
               type:PostalAddress
            type:Organization
            name:Harvard Medical School
            address:
               name:Department of Pediatrics, Harvard Medical School, Boston, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, USA
      name:Department of Pediatrics, Harvard Medical School, Boston, USA
      name:Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, USA
      name:Department of Pediatrics, Harvard Medical School, Boston, USA
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