We are analyzing https://link.springer.com/protocol/10.1007/978-1-4939-0329-0_14.

Title:
Four-Color FISH for the Detection of Low-Level Aneuploidy in Interphase Cells | SpringerLink
Description:
FISH (fluorescent in situ hybridization) is a molecular cytogenetic technique established in the early 1980s that allows for the detection of DNA copy number changes (gains and losses) mapping to genomic regions of interest (Langer-Safer et al. Proc Natl Acad Sci USA...
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month download article/chapter single-cell analysis low-frequency mosaic aneuploidy cold spring harbor privacy choices/manage cookies research-based investigations device instant download low-level aneuploidy editor information editors tumor necrosis factor metastatic breast cancer interphase cells protocol adult cerebellar cortex human genome browser european economic area situ hybridization langer-safer false-positive signals ccnd1 locus amplification aging mouse brain hanlon newell ae albert einstein college journal finder publish conditions privacy policy dna copy number chromosome-specific accumulation accepting optional cookies protocol faggioli drosophila polytene chromosomes p185her2 monoclonal antibody books a main content log usage analysis author correspondence protocol usd 49 affiliations department social media visual science permissions reprints interphase cells humana press cristina montagna check access ethics access protocol cite chapter log journal publish privacy policy color fish mammalian cells

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ScholarlyArticle:
      headline:Four-Color FISH for the Detection of Low-Level Aneuploidy in Interphase Cells
      pageEnd:305
      pageStart:291
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4939-0329-0.jpg
      genre:
         Springer Protocols
      isPartOf:
         name:Mitosis
         isbn:
            978-1-4939-0329-0
            978-1-4939-0328-3
         type:Book
      publisher:
         name:Springer New York
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Francesca Faggioli
            affiliation:
                  name:Albert Einstein College of Medicine, Yeshiva University
                  address:
                     name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jan Vijg
            affiliation:
                  name:Albert Einstein College of Medicine, Yeshiva University
                  address:
                     name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
                     type:PostalAddress
                  type:Organization
                  name:Albert Einstein College of Medicine, Yeshiva University
                  address:
                     name:Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
                     type:PostalAddress
                  type:Organization
                  name:Albert Einstein College of Medicine, Yeshiva University
                  address:
                     name:Ophthalmology and Visual Science, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
                     type:PostalAddress
                  type:Organization
                  name:Albert Einstein College of Medicine, Yeshiva University
                  address:
                     name:Obstetrics & Gynecology and Women’s Health, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Cristina Montagna
            affiliation:
                  name:Albert Einstein College of Medicine, Yeshiva University
                  address:
                     name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
                     type:PostalAddress
                  type:Organization
                  name:Albert Einstein College of Medicine, Yeshiva University
                  address:
                     name:Department of Pathology, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      keywords:FISH (fluorescent in situ hybridization), Fluorochromes, Aneuploidy, Interphase FISH, Chromosomes
      description:FISH (fluorescent in situ hybridization) is a molecular cytogenetic technique established in the early 1980s that allows for the detection of DNA copy number changes (gains and losses) mapping to genomic regions of interest (Langer-Safer et al. Proc Natl Acad Sci USA 79:4381–4385, 1982). This technology has been extensively applied to research-based investigations and is routinely used in prenatal diagnosis and oncology. Here we describe a modification of the standard FISH protocol adapted for the detection of low-frequency mosaic aneuploidy in interphase cells. This approach represents a straightforward method for the measurement of aneuploidy levels in mammalian cells. This system combines four probes mapping to two different chromosomes. The choice of probes is essential for the successful performance of this approach. It greatly reduces the enumeration of false-positive signals that are challenging in the enumeration of ploidy changes (particularly if these are complex and/or involve a significant increase of chromosome number).
      datePublished:2014
      isAccessibleForFree:
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         type:WebPageElement
      context:https://schema.org
Book:
      name:Mitosis
      isbn:
         978-1-4939-0329-0
         978-1-4939-0328-3
Organization:
      name:Springer New York
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Albert Einstein College of Medicine, Yeshiva University
      address:
         name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
         type:PostalAddress
      name:Albert Einstein College of Medicine, Yeshiva University
      address:
         name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
         type:PostalAddress
      name:Albert Einstein College of Medicine, Yeshiva University
      address:
         name:Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
         type:PostalAddress
      name:Albert Einstein College of Medicine, Yeshiva University
      address:
         name:Ophthalmology and Visual Science, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
         type:PostalAddress
      name:Albert Einstein College of Medicine, Yeshiva University
      address:
         name:Obstetrics & Gynecology and Women’s Health, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
         type:PostalAddress
      name:Albert Einstein College of Medicine, Yeshiva University
      address:
         name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
         type:PostalAddress
      name:Albert Einstein College of Medicine, Yeshiva University
      address:
         name:Department of Pathology, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Francesca Faggioli
      affiliation:
            name:Albert Einstein College of Medicine, Yeshiva University
            address:
               name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
               type:PostalAddress
            type:Organization
      name:Jan Vijg
      affiliation:
            name:Albert Einstein College of Medicine, Yeshiva University
            address:
               name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
               type:PostalAddress
            type:Organization
            name:Albert Einstein College of Medicine, Yeshiva University
            address:
               name:Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
               type:PostalAddress
            type:Organization
            name:Albert Einstein College of Medicine, Yeshiva University
            address:
               name:Ophthalmology and Visual Science, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
               type:PostalAddress
            type:Organization
            name:Albert Einstein College of Medicine, Yeshiva University
            address:
               name:Obstetrics & Gynecology and Women’s Health, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
               type:PostalAddress
            type:Organization
      name:Cristina Montagna
      affiliation:
            name:Albert Einstein College of Medicine, Yeshiva University
            address:
               name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
               type:PostalAddress
            type:Organization
            name:Albert Einstein College of Medicine, Yeshiva University
            address:
               name:Department of Pathology, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
      name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
      name:Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
      name:Ophthalmology and Visual Science, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
      name:Obstetrics & Gynecology and Women’s Health, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
      name:Department of Genetics, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
      name:Department of Pathology, Albert Einstein College of Medicine, Yeshiva University, Bronx, USA
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