We are analyzing https://link.springer.com/chapter/10.1007/978-1-4615-5865-1_45.

Title:
Different Enzyme Activities in Coronary Capillary Endothelial Cells | SpringerLink
Description:
Differential distributions of alkaline phosphatase (AP) and dipeptydylpeptidase IV (DPPIV) were studied in coronary microvascular endothelial cells. Endothelial cells were obtained by the perfusion of coronary vessels with 0.1% trypsin PBS solution and cultured in...
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Schema {🗺️}

ScholarlyArticle:
      headline:Different Enzyme Activities in Coronary Capillary Endothelial Cells
      pageEnd:364
      pageStart:359
      image:https://media.springernature.com/w153/springer-static/cover/book/978-1-4615-5865-1.jpg
      genre:
         Biomedical and Life Sciences
         Biomedical and Life Sciences (R0)
      isPartOf:
         name:Oxygen Transport to Tissue XVIII
         isbn:
            978-1-4615-5865-1
            978-1-4613-7689-7
         type:Book
      publisher:
         name:Springer US
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:T. Koyama
            affiliation:
                  name:Hokkaido University
                  address:
                     name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:M. Gao
            affiliation:
                  name:Hokkaido University
                  address:
                     name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:T. Ueda
            affiliation:
                  name:Hokkaido University
                  address:
                     name:Res Inst Immunol, Hokkaido University, Sapporo, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:S. Batra
            affiliation:
                  name:Hokkaido University
                  address:
                     name:JSPS researcher to Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:K. Itoh
            affiliation:
                  name:Hokkaido University
                  address:
                     name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:T. Ushiki
            affiliation:
                  name:Hokkaido University
                  address:
                     name:3rd Dept Anat Medical School, Hokkaido University, Sapporo, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
            name:K. Abe
            affiliation:
                  name:Hokkaido University
                  address:
                     name:3rd Dept Anat Medical School, Hokkaido University, Sapporo, Japan
                     type:PostalAddress
                  type:Organization
            type:Person
      keywords:Left Ventricular Wall, Capillary Endothelial Cell, Repeated Passage, Alkaline Phosphatase Staining, Colored Microsphere
      description:Differential distributions of alkaline phosphatase (AP) and dipeptydylpeptidase IV (DPPIV) were studied in coronary microvascular endothelial cells. Endothelial cells were obtained by the perfusion of coronary vessels with 0.1% trypsin PBS solution and cultured in uncoated culture dishes. Staining of cultured endothelial cells with AP- and DPPIVsensitive reagents revealed blue or red staining, respectively. Most colonies showed cells of only one color, blue or red, even at the fifth passage. AP-sensitive cells, which were originally elongated, shortened and widened, proliferating to form monolayer colonies of cobble stone-like cells. AP-stainability became weak with repeated passages. DPPIV-sensitive endothelial cells remained elongated even after repeated passages. The cell shape and stainability seemed to be coupled and maintained through the five passages studied.
      datePublished:1997
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Book:
      name:Oxygen Transport to Tissue XVIII
      isbn:
         978-1-4615-5865-1
         978-1-4613-7689-7
Organization:
      name:Springer US
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Hokkaido University
      address:
         name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
         type:PostalAddress
      name:Hokkaido University
      address:
         name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
         type:PostalAddress
      name:Hokkaido University
      address:
         name:Res Inst Immunol, Hokkaido University, Sapporo, Japan
         type:PostalAddress
      name:Hokkaido University
      address:
         name:JSPS researcher to Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
         type:PostalAddress
      name:Hokkaido University
      address:
         name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
         type:PostalAddress
      name:Hokkaido University
      address:
         name:3rd Dept Anat Medical School, Hokkaido University, Sapporo, Japan
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      name:Hokkaido University
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Person:
      name:T. Koyama
      affiliation:
            name:Hokkaido University
            address:
               name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
               type:PostalAddress
            type:Organization
      name:M. Gao
      affiliation:
            name:Hokkaido University
            address:
               name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
               type:PostalAddress
            type:Organization
      name:T. Ueda
      affiliation:
            name:Hokkaido University
            address:
               name:Res Inst Immunol, Hokkaido University, Sapporo, Japan
               type:PostalAddress
            type:Organization
      name:S. Batra
      affiliation:
            name:Hokkaido University
            address:
               name:JSPS researcher to Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
               type:PostalAddress
            type:Organization
      name:K. Itoh
      affiliation:
            name:Hokkaido University
            address:
               name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
               type:PostalAddress
            type:Organization
      name:T. Ushiki
      affiliation:
            name:Hokkaido University
            address:
               name:3rd Dept Anat Medical School, Hokkaido University, Sapporo, Japan
               type:PostalAddress
            type:Organization
      name:K. Abe
      affiliation:
            name:Hokkaido University
            address:
               name:3rd Dept Anat Medical School, Hokkaido University, Sapporo, Japan
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
      name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
      name:Res Inst Immunol, Hokkaido University, Sapporo, Japan
      name:JSPS researcher to Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
      name:Res Inst Electron Sc, Hokkaido University, Sapporo, Japan
      name:3rd Dept Anat Medical School, Hokkaido University, Sapporo, Japan
      name:3rd Dept Anat Medical School, Hokkaido University, Sapporo, Japan
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