We are analyzing https://link.springer.com/article/10.1007/s12307-018-0218-0.

Title:
Hypoxia Induces the Acquisition of Cancer Stem-like Phenotype Via Upregulation and Activation of Signal Transducer and Activator of Transcription-3 (STAT3) in MDA-MB-231, a Triple Negative Breast Cancer Cell Line | Cancer Microenvironment
Description:
The finding that hypoxia can induce cancer stemness in various experimental models is in agreement with the conceptual basis of cancer cell plasticity. Here, we aimed to gain insights into the molecular basis of hypoxia-induced cancer cell plasticity in triple negative breast cancer (TNBC). To achieve this goal, we employed our previously published in-vitro model of TNBC, in which a small subset of stem-like cells can be distinguished from the bulk cell population based on their responsiveness to a Sox2 reporter. In MDA-MB-231, a TNBC cell line, we observed that hypoxia significantly increased the expression of luciferase and green fluorescence protein (GFP), the readouts of the Sox2 reporter. Upon hypoxic challenge, the bulk, reporter unresponsive (RU) cells acquired stem-like features, as evidenced by the significant increases in the proportion of CD44high/CD24low cells, colony formation and resistance to cisplatin. Correlating with these phenotypic changes, RU cells exposed to hypoxia exhibited a substantial upregulation of the active/phosphorylated form of STAT3 (pSTAT3). This hypoxia-induced activation of STAT3 correlated with increased STAT3 transcriptional activity, as evidenced by increased STAT3-DNA binding and an altered gene expression profile. This hypoxia-induced STAT3 activation is biologically significant, since siRNA knockdown of STAT3 in RU cells significantly attenuated the hypoxia-induced acquisition of Sox2 activity and stem-like phenotypic features. In conclusion, our data have provided the proof-of-concept that STAT3 is a critical mediator in promoting the hypoxia-induced acquisition of cancer stemness in TNBC. Targeting STAT3 in TNBC may be useful in overcoming chemoresistance and decreasing the risk of disease relapse.
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Keywords {🔍}

cancer, article, google, scholar, cas, cell, cells, breast, stem, hypoxia, stat, wang, stemlike, triple, negative, expression, human, res, phenotype, gupta, lai, hypoxiainduced, zhang, research, activation, alshareef, data, stemness, plasticity, tnbc, sox, critical, sci, biol, alberta, manuscript, privacy, cookies, content, acquisition, mdamb, raymond, transcriptional, access, natl, tumor, growth, liu, myc, university,

Topics {✒️}

pi3k/akt/c-myc axis promotes il-6/gp130-induced premature senescence triple-negative breast cancer lentivirus-mediated hif-1alpha knockdown month download article/chapter ccl2 mediates cross-talk mediating hypoxia-induced chemoresistance hypoxia-related cisplatin resistance cancer cell plasticity cancer biology hypoxia-inducible factor -1alpha cancer stem cells increased stat3-dna binding cancer cell stemness hypoxia-induced stat3 activation growth factor-binding protein-5 cells acquired stem beta-catenin contributes breast tumor cells induce cancer stemness tnbc cell line stat3-igfbp5 axis full article pdf hypoxia significantly increased human breast tumours hypoxia-inducible factor 1 etoposide-induced apoptosis human astroglioma cells hypoxia-regulated delta privacy choices/manage cookies inhibits cell growth phenotypically diverse progeny ductal breast carcinoma hypoxia-induced activation cd44high/cd24low cells neoplastic nonstem cells hypoxia inducible factors hypoxia-induced acquisition intra-tumour heterogeneity mesenchymal transition ru cells exposed stem cells 7 stem cells 34 stem cells 30 stem cells 26 stem cells 20 human neuroblastoma cells hypoxia modifies proliferation cancer microenvironment 11 autocrine growth factor

Questions {❓}

Marusyk A, Almendro V, Polyak K (2012) Intra-tumour heterogeneity: a looking glass for cancer?

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         headline:Hypoxia Induces the Acquisition of Cancer Stem-like Phenotype Via Upregulation and Activation of Signal Transducer and Activator of Transcription-3 (STAT3) in MDA-MB-231, a Triple Negative Breast Cancer Cell Line
         description:The finding that hypoxia can induce cancer stemness in various experimental models is in agreement with the conceptual basis of cancer cell plasticity. Here, we aimed to gain insights into the molecular basis of hypoxia-induced cancer cell plasticity in triple negative breast cancer (TNBC). To achieve this goal, we employed our previously published in-vitro model of TNBC, in which a small subset of stem-like cells can be distinguished from the bulk cell population based on their responsiveness to a Sox2 reporter. In MDA-MB-231, a TNBC cell line, we observed that hypoxia significantly increased the expression of luciferase and green fluorescence protein (GFP), the readouts of the Sox2 reporter. Upon hypoxic challenge, the bulk, reporter unresponsive (RU) cells acquired stem-like features, as evidenced by the significant increases in the proportion of CD44high/CD24low cells, colony formation and resistance to cisplatin. Correlating with these phenotypic changes, RU cells exposed to hypoxia exhibited a substantial upregulation of the active/phosphorylated form of STAT3 (pSTAT3). This hypoxia-induced activation of STAT3 correlated with increased STAT3 transcriptional activity, as evidenced by increased STAT3-DNA binding and an altered gene expression profile. This hypoxia-induced STAT3 activation is biologically significant, since siRNA knockdown of STAT3 in RU cells significantly attenuated the hypoxia-induced acquisition of Sox2 activity and stem-like phenotypic features. In conclusion, our data have provided the proof-of-concept that STAT3 is a critical mediator in promoting the hypoxia-induced acquisition of cancer stemness in TNBC. Targeting STAT3 in TNBC may be useful in overcoming chemoresistance and decreasing the risk of disease relapse.
         datePublished:2018-09-25T00:00:00Z
         dateModified:2018-09-25T00:00:00Z
         pageStart:141
         pageEnd:152
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            STAT3
            Cancer cell plasticity
            Triple negative breast cancer
            Cancer stemness
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            Oncology
            Immunology
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      headline:Hypoxia Induces the Acquisition of Cancer Stem-like Phenotype Via Upregulation and Activation of Signal Transducer and Activator of Transcription-3 (STAT3) in MDA-MB-231, a Triple Negative Breast Cancer Cell Line
      description:The finding that hypoxia can induce cancer stemness in various experimental models is in agreement with the conceptual basis of cancer cell plasticity. Here, we aimed to gain insights into the molecular basis of hypoxia-induced cancer cell plasticity in triple negative breast cancer (TNBC). To achieve this goal, we employed our previously published in-vitro model of TNBC, in which a small subset of stem-like cells can be distinguished from the bulk cell population based on their responsiveness to a Sox2 reporter. In MDA-MB-231, a TNBC cell line, we observed that hypoxia significantly increased the expression of luciferase and green fluorescence protein (GFP), the readouts of the Sox2 reporter. Upon hypoxic challenge, the bulk, reporter unresponsive (RU) cells acquired stem-like features, as evidenced by the significant increases in the proportion of CD44high/CD24low cells, colony formation and resistance to cisplatin. Correlating with these phenotypic changes, RU cells exposed to hypoxia exhibited a substantial upregulation of the active/phosphorylated form of STAT3 (pSTAT3). This hypoxia-induced activation of STAT3 correlated with increased STAT3 transcriptional activity, as evidenced by increased STAT3-DNA binding and an altered gene expression profile. This hypoxia-induced STAT3 activation is biologically significant, since siRNA knockdown of STAT3 in RU cells significantly attenuated the hypoxia-induced acquisition of Sox2 activity and stem-like phenotypic features. In conclusion, our data have provided the proof-of-concept that STAT3 is a critical mediator in promoting the hypoxia-induced acquisition of cancer stemness in TNBC. Targeting STAT3 in TNBC may be useful in overcoming chemoresistance and decreasing the risk of disease relapse.
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      dateModified:2018-09-25T00:00:00Z
      pageStart:141
      pageEnd:152
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         STAT3
         Cancer cell plasticity
         Triple negative breast cancer
         Cancer stemness
         Cancer Research
         Oncology
         Immunology
         Cell Biology
         Biochemistry
         general
         Biomedicine
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            name:University of Alberta
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               name:Department of Oncology, University of Alberta, Edmonton, Canada
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