We are analyzing https://link.springer.com/article/10.1007/s10549-008-0174-3.

Title:
Evaluation of a RT-PCR based routine screening tool for the detection of disseminated epithelial cells in the bone marrow of breast cancer patients | Breast Cancer Research and Treatment
Description:
Purpose Numerous studies have shown that the presence of clinically occult disseminated tumor cells (DTC’s) in the bone marrow (BM) of breast cancer patients is associated with an unfavourable clinical outcome. Immunocytochemistry (ICC) remains the gold standard for their detection. While assays based on RT-PCR are available, they have not been used for routine detection of DTC’s. Methods To assess the quality of the assay, we performed a direct comparison of DTC detection rates in a large cohort of 385 patients using both standardized ICC and real-time RT-PCR protocols. Correlation rates were assessed, and results were compared with clinical data. Results A significant correlation between ICC and RT-PCR was observed (P < 0.01). Positivity rates were similar (both 35%) and the results of both methods agreed in 73% of cases (280/385). Conclusions We describe a real-time RT-PCR based protocol for DTC-detection that has been specifically designed for routine clinical laboratory use. As such, RT-PCR has the potential to become an alternative testing method for BM evaluation in breast cancer patients.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Health & Fitness
Education
Science

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 7,642,828 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure if the website is profiting.

Not all websites focus on profit; some are designed to educate, connect people, or share useful tools. People create websites for numerous reasons. And this could be one such example. Link.springer.com might be cashing in, but we can't detect the method they're using.

Keywords {🔍}

cancer, breast, google, scholar, pubmed, article, cas, cells, patients, detection, bone, marrow, clin, rtpcr, tumor, blood, oncol, circulating, disseminated, chain, reaction, realtime, res, polymerase, peripheral, clinical, prognostic, van, comparison, access, privacy, cookies, content, research, evaluation, epithelial, becker, solomayer, correlation, primary, reverse, int, quantitative, data, publish, search, based, routine, study, beckerpergola,

Topics {✒️}

real-time rt-pcr protocols real-time rt-pcr correlates real-time rt-pcr detection christina schuetz & tanja fehm month download article/chapter ck-19 mrna-positive cells high-risk breast cancer reverse transcription-pcr disseminated tumor cells cytokeratin 19 rt-pcr study tumor micrometastasis real-time quantification immunobead rt-pcr rt-pcr amplification full article pdf disseminated epithelial cells disseminated tumour cells cytokeratin-positive cells circulating tumor cells privacy choices/manage cookies assays based isolated tumor cells ck20 rt-pcr occult breast cancer occult metastatic cells micrometastatic breast cancer isolated micrometastatic cells immunomagnetic cell sorting circulating tumour cells circulating melanoma cells circulating epithelial cells bone marrow micrometastasis bone marrow aspirates early breast cancer iii breast cancer suspected breast cancer operable breast cancer metastatic breast cancer tumor cell detection related subjects diel ij negative immunomagnetic separation primary breast cancer bone marrow biopsy routine clinical laboratory blood/bone marrow breast cancer patients rt-pcr primary colorectal cancer european economic area

Questions {❓}

Are disseminated tumor cells in bone marrow and tumor-stroma ratio clinically applicable for patients undergoing surgical resection of primary colorectal cancer?

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Evaluation of a RT-PCR based routine screening tool for the detection of disseminated epithelial cells in the bone marrow of breast cancer patients
         description: Purpose Numerous studies have shown that the presence of clinically occult disseminated tumor cells (DTC’s) in the bone marrow (BM) of breast cancer patients is associated with an unfavourable clinical outcome. Immunocytochemistry (ICC) remains the gold standard for their detection. While assays based on RT-PCR are available, they have not been used for routine detection of DTC’s. Methods To assess the quality of the assay, we performed a direct comparison of DTC detection rates in a large cohort of 385 patients using both standardized ICC and real-time RT-PCR protocols. Correlation rates were assessed, and results were compared with clinical data. Results A significant correlation between ICC and RT-PCR was observed (P < 0.01). Positivity rates were similar (both 35%) and the results of both methods agreed in 73% of cases (280/385). Conclusions We describe a real-time RT-PCR based protocol for DTC-detection that has been specifically designed for routine clinical laboratory use. As such, RT-PCR has the potential to become an alternative testing method for BM evaluation in breast cancer patients.
         datePublished:2008-09-14T00:00:00Z
         dateModified:2008-09-14T00:00:00Z
         pageStart:227
         pageEnd:233
         sameAs:https://doi.org/10.1007/s10549-008-0174-3
         keywords:
            Disseminated tumor cells
            Immunocytochemistry
            Minimal residual disease
            Bone marrow
            Breast cancer
            RT-PCR
            Oncology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10549-008-0174-3/MediaObjects/10549_2008_174_Fig1_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10549-008-0174-3/MediaObjects/10549_2008_174_Fig2_HTML.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10549-008-0174-3/MediaObjects/10549_2008_174_Fig3_HTML.gif
         isPartOf:
            name:Breast Cancer Research and Treatment
            issn:
               1573-7217
               0167-6806
            volumeNumber:117
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer US
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Sven Becker
               affiliation:
                     name:University Hospital Tuebingen
                     address:
                        name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Graziella Becker-Pergola
               affiliation:
                     name:University Hospital Tuebingen
                     address:
                        name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Malgorzata Banys
               affiliation:
                     name:University Hospital Tuebingen
                     address:
                        name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Natalia Krawczyk
               affiliation:
                     name:University Hospital Tuebingen
                     address:
                        name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Diethelm Wallwiener
               affiliation:
                     name:University Hospital Tuebingen
                     address:
                        name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Erich Solomayer
               affiliation:
                     name:University Hospital Tuebingen
                     address:
                        name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Christina Schuetz
               affiliation:
                     name:University Hospital Tuebingen
                     address:
                        name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Tanja Fehm
               affiliation:
                     name:University Hospital Tuebingen
                     address:
                        name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Evaluation of a RT-PCR based routine screening tool for the detection of disseminated epithelial cells in the bone marrow of breast cancer patients
      description: Purpose Numerous studies have shown that the presence of clinically occult disseminated tumor cells (DTC’s) in the bone marrow (BM) of breast cancer patients is associated with an unfavourable clinical outcome. Immunocytochemistry (ICC) remains the gold standard for their detection. While assays based on RT-PCR are available, they have not been used for routine detection of DTC’s. Methods To assess the quality of the assay, we performed a direct comparison of DTC detection rates in a large cohort of 385 patients using both standardized ICC and real-time RT-PCR protocols. Correlation rates were assessed, and results were compared with clinical data. Results A significant correlation between ICC and RT-PCR was observed (P < 0.01). Positivity rates were similar (both 35%) and the results of both methods agreed in 73% of cases (280/385). Conclusions We describe a real-time RT-PCR based protocol for DTC-detection that has been specifically designed for routine clinical laboratory use. As such, RT-PCR has the potential to become an alternative testing method for BM evaluation in breast cancer patients.
      datePublished:2008-09-14T00:00:00Z
      dateModified:2008-09-14T00:00:00Z
      pageStart:227
      pageEnd:233
      sameAs:https://doi.org/10.1007/s10549-008-0174-3
      keywords:
         Disseminated tumor cells
         Immunocytochemistry
         Minimal residual disease
         Bone marrow
         Breast cancer
         RT-PCR
         Oncology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10549-008-0174-3/MediaObjects/10549_2008_174_Fig1_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10549-008-0174-3/MediaObjects/10549_2008_174_Fig2_HTML.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10549-008-0174-3/MediaObjects/10549_2008_174_Fig3_HTML.gif
      isPartOf:
         name:Breast Cancer Research and Treatment
         issn:
            1573-7217
            0167-6806
         volumeNumber:117
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer US
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Sven Becker
            affiliation:
                  name:University Hospital Tuebingen
                  address:
                     name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Graziella Becker-Pergola
            affiliation:
                  name:University Hospital Tuebingen
                  address:
                     name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Malgorzata Banys
            affiliation:
                  name:University Hospital Tuebingen
                  address:
                     name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Natalia Krawczyk
            affiliation:
                  name:University Hospital Tuebingen
                  address:
                     name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Diethelm Wallwiener
            affiliation:
                  name:University Hospital Tuebingen
                  address:
                     name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Erich Solomayer
            affiliation:
                  name:University Hospital Tuebingen
                  address:
                     name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Christina Schuetz
            affiliation:
                  name:University Hospital Tuebingen
                  address:
                     name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Tanja Fehm
            affiliation:
                  name:University Hospital Tuebingen
                  address:
                     name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Breast Cancer Research and Treatment
      issn:
         1573-7217
         0167-6806
      volumeNumber:117
Organization:
      name:Springer US
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University Hospital Tuebingen
      address:
         name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
         type:PostalAddress
      name:University Hospital Tuebingen
      address:
         name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
         type:PostalAddress
      name:University Hospital Tuebingen
      address:
         name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
         type:PostalAddress
      name:University Hospital Tuebingen
      address:
         name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
         type:PostalAddress
      name:University Hospital Tuebingen
      address:
         name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
         type:PostalAddress
      name:University Hospital Tuebingen
      address:
         name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
         type:PostalAddress
      name:University Hospital Tuebingen
      address:
         name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
         type:PostalAddress
      name:University Hospital Tuebingen
      address:
         name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Sven Becker
      affiliation:
            name:University Hospital Tuebingen
            address:
               name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Graziella Becker-Pergola
      affiliation:
            name:University Hospital Tuebingen
            address:
               name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
               type:PostalAddress
            type:Organization
      name:Malgorzata Banys
      affiliation:
            name:University Hospital Tuebingen
            address:
               name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
               type:PostalAddress
            type:Organization
      name:Natalia Krawczyk
      affiliation:
            name:University Hospital Tuebingen
            address:
               name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
               type:PostalAddress
            type:Organization
      name:Diethelm Wallwiener
      affiliation:
            name:University Hospital Tuebingen
            address:
               name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
               type:PostalAddress
            type:Organization
      name:Erich Solomayer
      affiliation:
            name:University Hospital Tuebingen
            address:
               name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
               type:PostalAddress
            type:Organization
      name:Christina Schuetz
      affiliation:
            name:University Hospital Tuebingen
            address:
               name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
               type:PostalAddress
            type:Organization
      name:Tanja Fehm
      affiliation:
            name:University Hospital Tuebingen
            address:
               name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
               type:PostalAddress
            type:Organization
PostalAddress:
      name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
      name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
      name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
      name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
      name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
      name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
      name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
      name:Department of Gynaecology and Obstetrics, University Hospital Tuebingen, Tuebingen, Germany
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(165)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

4.27s.

LINK . SPRINGER . COM {}