We are analyzing https://link.springer.com/article/10.1007/s10068-020-00821-8.

Title:
Characterization of recombinant protein ferritin from Pyropia yezoensis (rPyFer) and its biological activities | Food Science and Biotechnology
Description:
Ferritins are iron-binding proteins that are basically participated in iron storage, detoxification, and immune response. In the present study, ferritin gene from the marine red algae Pyropia yezoensis was cloned into a pET21d expression vector. High-efficiency transformation was performed in Escherichia coli BL21, the recombinant protein was expressed by induction with 0.1 mM isopropyl-β-D-thiogalactoside and purified via ammonium sulfate precipitation, anion exchange and size exclusion chromatography. The purified recombinant ferritin from P. yezoensis (rPyFer) was characterized and analyzed for its antimicrobial activity against both Gram-negative and Gram-positive bacterial cultures and exhibited significant antibacterial activity against Gram-positive cultures. The recombinant protein was also analyzed for its iron-uptake and radical-scavenging activities; rPyFer exhibited significant iron-uptake activity at low concentrations, and its radical-scavenging activity increased in a dose-dependent manner. This research will contribute to the development of new therapeutic proteins from marine algae.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Science
Non-Profit & Charity

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We're unsure how the site profits.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Link.springer.com could have a money-making trick up its sleeve, but it's undetectable for now.

Keywords {🔍}

article, google, scholar, cas, ferritin, iron, protein, activity, recombinant, expression, content, research, choi, characterization, yezoensis, marine, access, zhang, privacy, cookies, pyropia, proteins, algae, antioxidant, fish, information, publish, search, food, rpyfer, younhee, ferritins, storage, response, gene, coli, biochem, shellfish, immunol, magnesium, springer, data, log, journal, science, biological, activities, published, october, ulagesan,

Topics {✒️}

1 mm isopropyl-β-d-thiogalactoside minimum inhibitory concentrations month download article/chapter youn-hee choi radical-scavenging activity increased iron-binding proteins recombinant protein ferritin recombinant h-chain ferritins marine brown algae gram-positive bacterial cultures related subjects human ferritin l-chain h-chain deletion mutants antioxidant activity applying article food science jeong-wook choi smith jm therapeutic proteins recombinant protein iron-binding protein antioxidant response element marine algae marine bio-materials full article pdf purified recombinant ferritin privacy choices/manage cookies pyropia yezoensis ferro-oxidase activity radical-scavenging activities gram-positive cultures free radical bio antioxidant activity national research foundation check access instant access ferritin subunits involved blue-green alga ferritin iron uptake recombinant ferritin pet21d expression vector european economic area ammonium sulfate precipitation size exclusion chromatography dose-dependent manner ferric reducing ability heat shock method iptg inducible promoters chlamydomonas reinhardtii chloroplasts potential paleoceanographic applications t7 rna polymerase

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Characterization of recombinant protein ferritin from Pyropia yezoensis (rPyFer) and its biological activities
         description:Ferritins are iron-binding proteins that are basically participated in iron storage, detoxification, and immune response. In the present study, ferritin gene from the marine red algae Pyropia yezoensis was cloned into a pET21d expression vector. High-efficiency transformation was performed in Escherichia coli BL21, the recombinant protein was expressed by induction with 0.1 mM isopropyl-β-D-thiogalactoside and purified via ammonium sulfate precipitation, anion exchange and size exclusion chromatography. The purified recombinant ferritin from P. yezoensis (rPyFer) was characterized and analyzed for its antimicrobial activity against both Gram-negative and Gram-positive bacterial cultures and exhibited significant antibacterial activity against Gram-positive cultures. The recombinant protein was also analyzed for its iron-uptake and radical-scavenging activities; rPyFer exhibited significant iron-uptake activity at low concentrations, and its radical-scavenging activity increased in a dose-dependent manner. This research will contribute to the development of new therapeutic proteins from marine algae.
         datePublished:2020-10-06T00:00:00Z
         dateModified:2020-10-06T00:00:00Z
         pageStart:1501
         pageEnd:1509
         sameAs:https://doi.org/10.1007/s10068-020-00821-8
         keywords:
            Recombinant protein
             Pyropia yezoensis
            Ferritin
            Equine spleen ferritin
            Marine algae
            Antioxidant activity
            Minimum inhibitory concentration
            Food Science
            Nutrition
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10068-020-00821-8/MediaObjects/10068_2020_821_Fig1_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10068-020-00821-8/MediaObjects/10068_2020_821_Fig2_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10068-020-00821-8/MediaObjects/10068_2020_821_Fig3_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10068-020-00821-8/MediaObjects/10068_2020_821_Fig4_HTML.png
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10068-020-00821-8/MediaObjects/10068_2020_821_Fig5_HTML.png
         isPartOf:
            name:Food Science and Biotechnology
            issn:
               2092-6456
               1226-7708
            volumeNumber:29
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer Singapore
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Selvakumari Ulagesan
               affiliation:
                     name:Pukyong National University
                     address:
                        name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Jeong-Wook Choi
               affiliation:
                     name:Pukyong National University
                     address:
                        name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Taek-Jeong Nam
               affiliation:
                     name:Pukyong National University
                     address:
                        name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Youn-Hee Choi
               url:http://orcid.org/0000-0002-0091-7893
               affiliation:
                     name:Pukyong National University
                     address:
                        name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
                        type:PostalAddress
                     type:Organization
                     name:Pukyong National University
                     address:
                        name:Department of Marine Bio-materials and Aquaculture, Pukyong National University, Busan, Republic of Korea
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Characterization of recombinant protein ferritin from Pyropia yezoensis (rPyFer) and its biological activities
      description:Ferritins are iron-binding proteins that are basically participated in iron storage, detoxification, and immune response. In the present study, ferritin gene from the marine red algae Pyropia yezoensis was cloned into a pET21d expression vector. High-efficiency transformation was performed in Escherichia coli BL21, the recombinant protein was expressed by induction with 0.1 mM isopropyl-β-D-thiogalactoside and purified via ammonium sulfate precipitation, anion exchange and size exclusion chromatography. The purified recombinant ferritin from P. yezoensis (rPyFer) was characterized and analyzed for its antimicrobial activity against both Gram-negative and Gram-positive bacterial cultures and exhibited significant antibacterial activity against Gram-positive cultures. The recombinant protein was also analyzed for its iron-uptake and radical-scavenging activities; rPyFer exhibited significant iron-uptake activity at low concentrations, and its radical-scavenging activity increased in a dose-dependent manner. This research will contribute to the development of new therapeutic proteins from marine algae.
      datePublished:2020-10-06T00:00:00Z
      dateModified:2020-10-06T00:00:00Z
      pageStart:1501
      pageEnd:1509
      sameAs:https://doi.org/10.1007/s10068-020-00821-8
      keywords:
         Recombinant protein
          Pyropia yezoensis
         Ferritin
         Equine spleen ferritin
         Marine algae
         Antioxidant activity
         Minimum inhibitory concentration
         Food Science
         Nutrition
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10068-020-00821-8/MediaObjects/10068_2020_821_Fig1_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10068-020-00821-8/MediaObjects/10068_2020_821_Fig2_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10068-020-00821-8/MediaObjects/10068_2020_821_Fig3_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10068-020-00821-8/MediaObjects/10068_2020_821_Fig4_HTML.png
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs10068-020-00821-8/MediaObjects/10068_2020_821_Fig5_HTML.png
      isPartOf:
         name:Food Science and Biotechnology
         issn:
            2092-6456
            1226-7708
         volumeNumber:29
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer Singapore
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Selvakumari Ulagesan
            affiliation:
                  name:Pukyong National University
                  address:
                     name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Jeong-Wook Choi
            affiliation:
                  name:Pukyong National University
                  address:
                     name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Taek-Jeong Nam
            affiliation:
                  name:Pukyong National University
                  address:
                     name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Youn-Hee Choi
            url:http://orcid.org/0000-0002-0091-7893
            affiliation:
                  name:Pukyong National University
                  address:
                     name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
                     type:PostalAddress
                  type:Organization
                  name:Pukyong National University
                  address:
                     name:Department of Marine Bio-materials and Aquaculture, Pukyong National University, Busan, Republic of Korea
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Food Science and Biotechnology
      issn:
         2092-6456
         1226-7708
      volumeNumber:29
Organization:
      name:Springer Singapore
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:Pukyong National University
      address:
         name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
         type:PostalAddress
      name:Pukyong National University
      address:
         name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
         type:PostalAddress
      name:Pukyong National University
      address:
         name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
         type:PostalAddress
      name:Pukyong National University
      address:
         name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
         type:PostalAddress
      name:Pukyong National University
      address:
         name:Department of Marine Bio-materials and Aquaculture, Pukyong National University, Busan, Republic of Korea
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Selvakumari Ulagesan
      affiliation:
            name:Pukyong National University
            address:
               name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
               type:PostalAddress
            type:Organization
      name:Jeong-Wook Choi
      affiliation:
            name:Pukyong National University
            address:
               name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
               type:PostalAddress
            type:Organization
      name:Taek-Jeong Nam
      affiliation:
            name:Pukyong National University
            address:
               name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
               type:PostalAddress
            type:Organization
      name:Youn-Hee Choi
      url:http://orcid.org/0000-0002-0091-7893
      affiliation:
            name:Pukyong National University
            address:
               name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
               type:PostalAddress
            type:Organization
            name:Pukyong National University
            address:
               name:Department of Marine Bio-materials and Aquaculture, Pukyong National University, Busan, Republic of Korea
               type:PostalAddress
            type:Organization
      email:[email protected]
PostalAddress:
      name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
      name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
      name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
      name:Institute of Fisheries Sciences, Pukyong National University, Busan, Republic of Korea
      name:Department of Marine Bio-materials and Aquaculture, Pukyong National University, Busan, Republic of Korea
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(97)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

CDN Services {📦}

Crossref

3.91s.

LINK . SPRINGER . COM {}