We are analyzing https://link.springer.com/article/10.1007/s00424-013-1342-9.

Title:
Ion transporters in brain endothelial cells that contribute to formation of brain interstitial fluid | Pflügers Archiv - European Journal of Physiology
Description:
Ions and water transported across the endothelium lining the blood–brain barrier contribute to the fluid secreted into the brain and are important in maintaining appropriate volume and ionic composition of brain interstitial fluid. Changes in this secretion process may occur after stroke. The present study identifies at transcript and protein level ion transporters involved in the movement of key ions and examines how levels of certain of these alter following oxidative stress. Immunohistochemistry provides evidence for Cl−/HCO3 − exchanger, AE2, and Na+, HCO3 − cotransporters, NBCe1 and NBCn1, on brain microvessels. mRNA analysis by RT-PCR reveals expression of these transporters in cultured rat brain microvascular endothelial cells (both primary and immortalized GPNT cells) and also Na+/H+ exchangers, NHE1 (primary and immortalized) and NHE2 (primary cells only). Knock-down using siRNA in immortalized GPNT cells identifies AE2 as responsible for much of the Cl−/HCO3 − exchange following extracellular chloride removal and NHE1 as the transporter that accounts for most of the Na+/H+ exchange following intracellular acidification. Transcript levels of both AE2 and NHE1 are increased following hypoxia/reoxygenation. Further work is now required to determine the localization of the bicarbonate transporters to luminal or abluminal membranes of the endothelial cells as well as to identify and localize additional transport mechanisms that must exist for K+ and Cl−.
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Keywords {🔍}

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Topics {✒️}

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Schema {🗺️}

WebPage:
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         headline:Ion transporters in brain endothelial cells that contribute to formation of brain interstitial fluid
         description:Ions and water transported across the endothelium lining the blood–brain barrier contribute to the fluid secreted into the brain and are important in maintaining appropriate volume and ionic composition of brain interstitial fluid. Changes in this secretion process may occur after stroke. The present study identifies at transcript and protein level ion transporters involved in the movement of key ions and examines how levels of certain of these alter following oxidative stress. Immunohistochemistry provides evidence for Cl−/HCO3 − exchanger, AE2, and Na+, HCO3 − cotransporters, NBCe1 and NBCn1, on brain microvessels. mRNA analysis by RT-PCR reveals expression of these transporters in cultured rat brain microvascular endothelial cells (both primary and immortalized GPNT cells) and also Na+/H+ exchangers, NHE1 (primary and immortalized) and NHE2 (primary cells only). Knock-down using siRNA in immortalized GPNT cells identifies AE2 as responsible for much of the Cl−/HCO3 − exchange following extracellular chloride removal and NHE1 as the transporter that accounts for most of the Na+/H+ exchange following intracellular acidification. Transcript levels of both AE2 and NHE1 are increased following hypoxia/reoxygenation. Further work is now required to determine the localization of the bicarbonate transporters to luminal or abluminal membranes of the endothelial cells as well as to identify and localize additional transport mechanisms that must exist for K+ and Cl−.
         datePublished:2013-09-11T00:00:00Z
         dateModified:2013-09-11T00:00:00Z
         pageStart:887
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            Blood–brain barrier
            Brain interstitial fluid
            Cl−/HCO3 − exchanger AE2
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            Na+/H+ exchangers NHE1 and NHE2
            Human Physiology
            Molecular Medicine
            Neurosciences
            Cell Biology
            Receptors
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ScholarlyArticle:
      headline:Ion transporters in brain endothelial cells that contribute to formation of brain interstitial fluid
      description:Ions and water transported across the endothelium lining the blood–brain barrier contribute to the fluid secreted into the brain and are important in maintaining appropriate volume and ionic composition of brain interstitial fluid. Changes in this secretion process may occur after stroke. The present study identifies at transcript and protein level ion transporters involved in the movement of key ions and examines how levels of certain of these alter following oxidative stress. Immunohistochemistry provides evidence for Cl−/HCO3 − exchanger, AE2, and Na+, HCO3 − cotransporters, NBCe1 and NBCn1, on brain microvessels. mRNA analysis by RT-PCR reveals expression of these transporters in cultured rat brain microvascular endothelial cells (both primary and immortalized GPNT cells) and also Na+/H+ exchangers, NHE1 (primary and immortalized) and NHE2 (primary cells only). Knock-down using siRNA in immortalized GPNT cells identifies AE2 as responsible for much of the Cl−/HCO3 − exchange following extracellular chloride removal and NHE1 as the transporter that accounts for most of the Na+/H+ exchange following intracellular acidification. Transcript levels of both AE2 and NHE1 are increased following hypoxia/reoxygenation. Further work is now required to determine the localization of the bicarbonate transporters to luminal or abluminal membranes of the endothelial cells as well as to identify and localize additional transport mechanisms that must exist for K+ and Cl−.
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      dateModified:2013-09-11T00:00:00Z
      pageStart:887
      pageEnd:901
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         Rat brain endothelial cells
         Blood–brain barrier
         Brain interstitial fluid
         Cl−/HCO3 − exchanger AE2
         Na+, HCO3 − cotransporters, NBCe1 and NBCn1
         Na+/H+ exchangers NHE1 and NHE2
         Human Physiology
         Molecular Medicine
         Neurosciences
         Cell Biology
         Receptors
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