We are analyzing https://link.springer.com/article/10.1007/s00251-003-0621-6.

Title:
Alleles carried at positions −819 and −592 of the IL10 promoter affect transcription following stimulation of peripheral blood cells with Streptococcus pneumoniae | Immunogenetics
Description:
IL-10 inhibits the production of many pro-inflammatory cytokines. Polymorphisms in the IL10 gene promoter at positions −1082G→A, −819C→T and −592C→A occur as three haplotypes, ATA, GCC and ACC. These influence several infectious and inflammatory diseases including community-acquired pneumonia, where a role for IL-10 is suggested by fluctuations in plasma levels of the cytokine. However, the effects of the haplotypes on IL-10 production are unclear. We stimulated peripheral blood mononuclear cells (PBMC) from at least five individuals homozygous for each of the three haplotypes with lipopolysaccharide (LPS, 10 μg/ml) or heat-killed Streptococcus pneumoniae (107cfu/ml) and measured IL-10 mRNA by RT-PCR. Following S. pneumoniae stimulation, PBMC with the ATA haplotype had higher IL-10 mRNA levels than those with the GCC haplotype at 4 h (independent t-test; P=0.024), or the ACC haplotype at 4 h (P<0.0001) and 8 h (P=0.007). Following LPS stimulation, IL-10 mRNA levels were not significantly influenced by the IL10 haplotype, but similar trends were observed, consistent with the variable outcome of published studies. The results suggest that the −819 and/or −592 alleles affect transcription.
Website Age:
28 years and 1 months (reg. 1997-05-29).

Matching Content Categories {📚}

Education
Video & Online Content
Social Networks

Content Management System {📝}

What CMS is link.springer.com built with?

Custom-built

No common CMS systems were detected on Link.springer.com, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of link.springer.com audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month

Based on our best estimate, this website will receive around 5,000,016 visitors per month in the current month.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Link.springer.com Make Money? {💸}

We find it hard to spot revenue streams.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Link.springer.com might be plotting its profit, but the way they're doing it isn't detectable yet.

Keywords {🔍}

article, google, scholar, pubmed, cas, interleukin, promoter, gene, production, polymorphisms, haplotypes, transcription, australia, cytokine, polymorphism, huizinga, perth, privacy, cookies, content, research, pneumoniae, temple, inflammatory, haplotype, access, hutchinson, arthritis, westendorp, human, infect, dis, western, publish, search, immunogenetics, alleles, affect, stimulation, streptococcus, cheong, price, influence, pneumonia, levels, mrna, syndrome, association, keijsers, usa,

Topics {✒️}

monocyte/macrophage-induced inflammatory response month download article/chapter heat-killed streptococcus pneumoniae maldi-tof mass spectrometry tumor necrosis factor-alpha article immunogenetics aims anti-inflammatory cytokine production full article pdf related subjects interleukin-10 gene promoter il10 gene promoter privacy choices/manage cookies community acquired pneumonia community-acquired pneumonia peripheral blood cells inflammatory bowel diseases lymphoma cell lines il10 promoter variants single nucleotide polymorphisms european economic area pro-inflammatory cytokines independent t-test systemic lupus erythematosus gpo box x2213 inflammatory bowel disease experimental human endotoxemia alleles carried conditions privacy policy measured il-10 mrna fc gamma receptor gram-negative bacteria inhibits cytokine synthesis il-10 mrna levels major haplotype families western australian institute medical research de straat fg article temple accepting optional cookies streptococcus pneumoniae article log pneumococcal septic shock hajeer ah royal perth hospital tnf alpha author information authors de winkel jg de vries je check access instant access

Schema {🗺️}

WebPage:
      mainEntity:
         headline:Alleles carried at positions −819 and −592 of the IL10 promoter affect transcription following stimulation of peripheral blood cells with Streptococcus pneumoniae
         description:IL-10 inhibits the production of many pro-inflammatory cytokines. Polymorphisms in the IL10 gene promoter at positions −1082G→A, −819C→T and −592C→A occur as three haplotypes, ATA, GCC and ACC. These influence several infectious and inflammatory diseases including community-acquired pneumonia, where a role for IL-10 is suggested by fluctuations in plasma levels of the cytokine. However, the effects of the haplotypes on IL-10 production are unclear. We stimulated peripheral blood mononuclear cells (PBMC) from at least five individuals homozygous for each of the three haplotypes with lipopolysaccharide (LPS, 10 μg/ml) or heat-killed Streptococcus pneumoniae (107cfu/ml) and measured IL-10 mRNA by RT-PCR. Following S. pneumoniae stimulation, PBMC with the ATA haplotype had higher IL-10 mRNA levels than those with the GCC haplotype at 4 h (independent t-test; P=0.024), or the ACC haplotype at 4 h (P<0.0001) and 8 h (P=0.007). Following LPS stimulation, IL-10 mRNA levels were not significantly influenced by the IL10 haplotype, but similar trends were observed, consistent with the variable outcome of published studies. The results suggest that the −819 and/or −592 alleles affect transcription.
         datePublished:2003-11-06T00:00:00Z
         dateModified:2003-11-06T00:00:00Z
         pageStart:629
         pageEnd:632
         sameAs:https://doi.org/10.1007/s00251-003-0621-6
         keywords:
            Interleukin 10
            Lipopolysaccharide
             Streptococcus pneumoniae
            Promoter polymorphisms
            Immunology
            Human Genetics
            Gene Function
            Cell Biology
            Allergology
         image:
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00251-003-0621-6/MediaObjects/s00251-003-0621-6flb1.gif
            https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00251-003-0621-6/MediaObjects/s00251-003-0621-6flb2a-b.gif
         isPartOf:
            name:Immunogenetics
            issn:
               1432-1211
               0093-7711
            volumeNumber:55
            type:
               Periodical
               PublicationVolume
         publisher:
            name:Springer-Verlag
            logo:
               url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
               type:ImageObject
            type:Organization
         author:
               name:Suzanna E. L. Temple
               affiliation:
                     name:University of Western Australia
                     address:
                        name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
                        type:PostalAddress
                     type:Organization
               email:[email protected]
               type:Person
               name:Esther Lim
               affiliation:
                     name:University of Western Australia
                     address:
                        name:Department of Pathology, Royal Perth Hospital, University of Western Australia, Perth, Australia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Karey Y. Cheong
               affiliation:
                     name:University of Western Australia
                     address:
                        name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Coral-Ann M. Almeida
               affiliation:
                     name:University of Western Australia
                     address:
                        name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Patricia Price
               affiliation:
                     name:University of Western Australia
                     address:
                        name:Department of Clinical Immunology and Biochemical Genetics, School of Surgery and Pathology, Royal Perth Hospital, University of Western Australia, Perth, Australia
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Kristin G. Ardlie
               affiliation:
                     name:Genomics Collaborative Inc.
                     address:
                        name:Genomics Collaborative Inc., Cambridge, USA
                        type:PostalAddress
                     type:Organization
               type:Person
               name:Grant W. Waterer
               affiliation:
                     name:University of Western Australia
                     address:
                        name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
                        type:PostalAddress
                     type:Organization
               type:Person
         isAccessibleForFree:
         hasPart:
            isAccessibleForFree:
            cssSelector:.main-content
            type:WebPageElement
         type:ScholarlyArticle
      context:https://schema.org
ScholarlyArticle:
      headline:Alleles carried at positions −819 and −592 of the IL10 promoter affect transcription following stimulation of peripheral blood cells with Streptococcus pneumoniae
      description:IL-10 inhibits the production of many pro-inflammatory cytokines. Polymorphisms in the IL10 gene promoter at positions −1082G→A, −819C→T and −592C→A occur as three haplotypes, ATA, GCC and ACC. These influence several infectious and inflammatory diseases including community-acquired pneumonia, where a role for IL-10 is suggested by fluctuations in plasma levels of the cytokine. However, the effects of the haplotypes on IL-10 production are unclear. We stimulated peripheral blood mononuclear cells (PBMC) from at least five individuals homozygous for each of the three haplotypes with lipopolysaccharide (LPS, 10 μg/ml) or heat-killed Streptococcus pneumoniae (107cfu/ml) and measured IL-10 mRNA by RT-PCR. Following S. pneumoniae stimulation, PBMC with the ATA haplotype had higher IL-10 mRNA levels than those with the GCC haplotype at 4 h (independent t-test; P=0.024), or the ACC haplotype at 4 h (P<0.0001) and 8 h (P=0.007). Following LPS stimulation, IL-10 mRNA levels were not significantly influenced by the IL10 haplotype, but similar trends were observed, consistent with the variable outcome of published studies. The results suggest that the −819 and/or −592 alleles affect transcription.
      datePublished:2003-11-06T00:00:00Z
      dateModified:2003-11-06T00:00:00Z
      pageStart:629
      pageEnd:632
      sameAs:https://doi.org/10.1007/s00251-003-0621-6
      keywords:
         Interleukin 10
         Lipopolysaccharide
          Streptococcus pneumoniae
         Promoter polymorphisms
         Immunology
         Human Genetics
         Gene Function
         Cell Biology
         Allergology
      image:
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00251-003-0621-6/MediaObjects/s00251-003-0621-6flb1.gif
         https://media.springernature.com/lw1200/springer-static/image/art%3A10.1007%2Fs00251-003-0621-6/MediaObjects/s00251-003-0621-6flb2a-b.gif
      isPartOf:
         name:Immunogenetics
         issn:
            1432-1211
            0093-7711
         volumeNumber:55
         type:
            Periodical
            PublicationVolume
      publisher:
         name:Springer-Verlag
         logo:
            url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
            type:ImageObject
         type:Organization
      author:
            name:Suzanna E. L. Temple
            affiliation:
                  name:University of Western Australia
                  address:
                     name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
                     type:PostalAddress
                  type:Organization
            email:[email protected]
            type:Person
            name:Esther Lim
            affiliation:
                  name:University of Western Australia
                  address:
                     name:Department of Pathology, Royal Perth Hospital, University of Western Australia, Perth, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Karey Y. Cheong
            affiliation:
                  name:University of Western Australia
                  address:
                     name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Coral-Ann M. Almeida
            affiliation:
                  name:University of Western Australia
                  address:
                     name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Patricia Price
            affiliation:
                  name:University of Western Australia
                  address:
                     name:Department of Clinical Immunology and Biochemical Genetics, School of Surgery and Pathology, Royal Perth Hospital, University of Western Australia, Perth, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Kristin G. Ardlie
            affiliation:
                  name:Genomics Collaborative Inc.
                  address:
                     name:Genomics Collaborative Inc., Cambridge, USA
                     type:PostalAddress
                  type:Organization
            type:Person
            name:Grant W. Waterer
            affiliation:
                  name:University of Western Australia
                  address:
                     name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
                     type:PostalAddress
                  type:Organization
            type:Person
      isAccessibleForFree:
      hasPart:
         isAccessibleForFree:
         cssSelector:.main-content
         type:WebPageElement
["Periodical","PublicationVolume"]:
      name:Immunogenetics
      issn:
         1432-1211
         0093-7711
      volumeNumber:55
Organization:
      name:Springer-Verlag
      logo:
         url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
         type:ImageObject
      name:University of Western Australia
      address:
         name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
         type:PostalAddress
      name:University of Western Australia
      address:
         name:Department of Pathology, Royal Perth Hospital, University of Western Australia, Perth, Australia
         type:PostalAddress
      name:University of Western Australia
      address:
         name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
         type:PostalAddress
      name:University of Western Australia
      address:
         name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
         type:PostalAddress
      name:University of Western Australia
      address:
         name:Department of Clinical Immunology and Biochemical Genetics, School of Surgery and Pathology, Royal Perth Hospital, University of Western Australia, Perth, Australia
         type:PostalAddress
      name:Genomics Collaborative Inc.
      address:
         name:Genomics Collaborative Inc., Cambridge, USA
         type:PostalAddress
      name:University of Western Australia
      address:
         name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
         type:PostalAddress
ImageObject:
      url:https://www.springernature.com/app-sn/public/images/logo-springernature.png
Person:
      name:Suzanna E. L. Temple
      affiliation:
            name:University of Western Australia
            address:
               name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
               type:PostalAddress
            type:Organization
      email:[email protected]
      name:Esther Lim
      affiliation:
            name:University of Western Australia
            address:
               name:Department of Pathology, Royal Perth Hospital, University of Western Australia, Perth, Australia
               type:PostalAddress
            type:Organization
      name:Karey Y. Cheong
      affiliation:
            name:University of Western Australia
            address:
               name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
               type:PostalAddress
            type:Organization
      name:Coral-Ann M. Almeida
      affiliation:
            name:University of Western Australia
            address:
               name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
               type:PostalAddress
            type:Organization
      name:Patricia Price
      affiliation:
            name:University of Western Australia
            address:
               name:Department of Clinical Immunology and Biochemical Genetics, School of Surgery and Pathology, Royal Perth Hospital, University of Western Australia, Perth, Australia
               type:PostalAddress
            type:Organization
      name:Kristin G. Ardlie
      affiliation:
            name:Genomics Collaborative Inc.
            address:
               name:Genomics Collaborative Inc., Cambridge, USA
               type:PostalAddress
            type:Organization
      name:Grant W. Waterer
      affiliation:
            name:University of Western Australia
            address:
               name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
               type:PostalAddress
            type:Organization
PostalAddress:
      name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
      name:Department of Pathology, Royal Perth Hospital, University of Western Australia, Perth, Australia
      name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
      name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
      name:Department of Clinical Immunology and Biochemical Genetics, School of Surgery and Pathology, Royal Perth Hospital, University of Western Australia, Perth, Australia
      name:Genomics Collaborative Inc., Cambridge, USA
      name:School of Medicine and Pharmacology, Western Australian Institute for Medical Research, Royal Perth Hospital, University of Western Australia, Perth, Australia
WebPageElement:
      isAccessibleForFree:
      cssSelector:.main-content

External Links {🔗}(100)

Analytics and Tracking {📊}

Google Tag Manager

Libraries {📚}

Clipboard.js
Prism.js

4.26s.

LINK . SPRINGER . COM {}