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Title:
Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10 | Cellular and Molecular Life Sciences
Description:
Mesenchymal stem cells (MSCs) have been shown to communicate with tumor cells. We analyzed the effect of human MSCs (hMSCs) on breast cancer cells in three-dimensional cultures. By using GFP expression and immunohistochemistry, we show that hMSCs invade 3D breast cancer cell aggregates. hMSCs caused breast cancer spheroids to become disorganized which was accompanied by a disruption of cellâcell adhesion, E-cadherin cleavage, and nuclear translocation of E-cadherin, but not by epithelial/mesenchymal transition or by an increase in ERK1/2 activity. In addition, hMSCs enhanced the motility of breast cancer cells. Inhibition of ADAM10 (a disintegrin and metalloprotease 10), known to cleave E-cadherin, prevented both hMSC-mediated E-cadherin cleavage and enhanced migration. Our data suggest that hMSCs interfere with cellâcell adhesion and enhance migration of breast cancer cells by activating ADAM10.
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Keywords {đ}
cells, google, scholar, article, cancer, pubmed, cas, mesenchymal, breast, stem, human, cell, ecadherin, dittmer, biol, adam, migration, access, res, chem, privacy, cookies, content, tumor, hmscs, epithelial, mol, doijbcm, data, publish, research, search, spheroids, cellcell, open, marini, sci, halle, information, log, journal, cellular, life, carcinoma, activating, hohlfeld, lĂźtzkendorf, jĂźrgen, adhesion, discover,
Topics {âď¸}
epithelial/mesenchymal transition hmsc-mediated e-cadherin cleavage mesenchymal stem cells month download article/chapter mesenchymal stromal cells human bone marrow elevated sdf-1/cxcl12 secretion breast cancer cells breast tumor cells related subjects stem cells 24 fibroblast-led collective invasion breast carcinoma spheroids e-cadherin cleavage full article pdf adam10-mediated release cleaved cytoplasmic domain egf receptor transactivation stromal fibroblasts present cleave e-cadherin generation lentivirus vector high-efficiency transformation e-cadherin fragment e-cadherin molecule mda-mb-231 cells epithelial cells privacy choices/manage cookies protein-coupled receptors solid pseudopapillary tumour cancer cells check access instant access cellâcell adhesion egf induces macropinocytosis vitro propagation consistent features article cellular article dittmer tumor cells cellâcell organization cell migration european economic area e-cadherin conditional packaging system multidrug resistant variant induces luminal repopulation snx1-modulated recycling sustained erk signaling reliable loading control land sachsen-anhalt
Questions {â}
- Shchors K, Evan G (2007) Tumor angiogenesis: cause or consequence of cancer?
- Yen L, Yen M-L (2008) Mesenchymal stem cells and cancerâfor better or for worse?
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headline:Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10
description:Mesenchymal stem cells (MSCs) have been shown to communicate with tumor cells. We analyzed the effect of human MSCs (hMSCs) on breast cancer cells in three-dimensional cultures. By using GFP expression and immunohistochemistry, we show that hMSCs invade 3D breast cancer cell aggregates. hMSCs caused breast cancer spheroids to become disorganized which was accompanied by a disruption of cellâcell adhesion, E-cadherin cleavage, and nuclear translocation of E-cadherin, but not by epithelial/mesenchymal transition or by an increase in ERK1/2 activity. In addition, hMSCs enhanced the motility of breast cancer cells. Inhibition of ADAM10 (a disintegrin and metalloprotease 10), known to cleave E-cadherin, prevented both hMSC-mediated E-cadherin cleavage and enhanced migration. Our data suggest that hMSCs interfere with cellâcell adhesion and enhance migration of breast cancer cells by activating ADAM10.
datePublished:2009-07-15T00:00:00Z
dateModified:2009-07-15T00:00:00Z
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MSC
Spheroids
Breast cancer
Migration
ADAM 10
E-cadherin
Cell Biology
Biomedicine
general
Life Sciences
Biochemistry
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headline:Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10
description:Mesenchymal stem cells (MSCs) have been shown to communicate with tumor cells. We analyzed the effect of human MSCs (hMSCs) on breast cancer cells in three-dimensional cultures. By using GFP expression and immunohistochemistry, we show that hMSCs invade 3D breast cancer cell aggregates. hMSCs caused breast cancer spheroids to become disorganized which was accompanied by a disruption of cellâcell adhesion, E-cadherin cleavage, and nuclear translocation of E-cadherin, but not by epithelial/mesenchymal transition or by an increase in ERK1/2 activity. In addition, hMSCs enhanced the motility of breast cancer cells. Inhibition of ADAM10 (a disintegrin and metalloprotease 10), known to cleave E-cadherin, prevented both hMSC-mediated E-cadherin cleavage and enhanced migration. Our data suggest that hMSCs interfere with cellâcell adhesion and enhance migration of breast cancer cells by activating ADAM10.
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