We are analyzing https://link.springer.com/article/10.1007/bf02256116.

Title:
Deoxyribonuclease-Inhibitory antibodies in systemic lupus erythematosus | Journal of Biomedical Science
Description:
Deoxyribonucleases (DNases) are key enzymes for digesting DNA. Abnormalities in the function of these enzymes may contribute to the development of anti-DNA antibodies in systemic lupus erythematosus (SLE). In this study, we used bovine DNase 1-coated ELISA plates to screen anti-DNase antibodies in SLE patients. About 62% of the sera of SLE patients (63/101) were positive for anti-DNase antibodies compared to only 8% of normal controls (8/98). A positive correlation was also found between the concentrations of anti-DNase and anti-DNA antibodies in sera of SLE patients. Affinity-purified anti-DNase immunoglobulin G (IgG) from pooled sera of SLE patients bound to bovine DNase as well as DNA. A synthetic peptide, corresponding to the catalytic site of DNase, was able to completely inhibit the binding of anti-DNase IgG to DNase. In addition to bovine DNase, the anti-DNase IgG also bound to and inhibited the enzymatic activities of DNase present in streptococcal supernatants and human urine. Immunization of lupus-prone NZB/NZW mice with bovine DNase enhanced the production of anti-DNase and DNA antibodies, and accelerated the occurrence of proteinuria. Taken together, these results suggest that DNase-inhibitory antibodies which recognize a conserved epitope near the catalytic site of DNase may act in the pathogenesis of SLE.
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Keywords {🔍}

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Topics {✒️}

lupus-prone nzb/nzw mice affinity-purified anti-dnase immunoglobulin prone nzb/nzw mice anti-dna antibodies bind month download article/chapter sds-polyacrylamide gel electrophoresis screen anti-dnase antibodies anti-dnase antibodies compared dna hydrolyzing autoantibodies anti-dna antibodies systemic lupus erythematosus human serum deoxyribonu-clease related subjects anti-nuclear antibodies deoxyribonuclease-inhibitory antibodies privacy choices/manage cookies key enzymes hung-chuan chang chun-ching liang dnase-inhibitory antibodies full article pdf dna antibodies dna binding interface european economic area september 2003 volume 10 dnase1-deficient mice metal ion dependence pathological lymphocyte activation conditions privacy policy check access article yeh instant access uk subjects anti-dnase igg bovine dnase enhanced accepting optional cookies human pancreatic dnase journal finder publish catalytic site lupus patients article journal affiliations department digesting dna dna recognition cutting dna human urine dnase article log anti-dnase sle patients bound privacy policy

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      headline:Deoxyribonuclease-Inhibitory antibodies in systemic lupus erythematosus
      description:Deoxyribonucleases (DNases) are key enzymes for digesting DNA. Abnormalities in the function of these enzymes may contribute to the development of anti-DNA antibodies in systemic lupus erythematosus (SLE). In this study, we used bovine DNase 1-coated ELISA plates to screen anti-DNase antibodies in SLE patients. About 62% of the sera of SLE patients (63/101) were positive for anti-DNase antibodies compared to only 8% of normal controls (8/98). A positive correlation was also found between the concentrations of anti-DNase and anti-DNA antibodies in sera of SLE patients. Affinity-purified anti-DNase immunoglobulin G (IgG) from pooled sera of SLE patients bound to bovine DNase as well as DNA. A synthetic peptide, corresponding to the catalytic site of DNase, was able to completely inhibit the binding of anti-DNase IgG to DNase. In addition to bovine DNase, the anti-DNase IgG also bound to and inhibited the enzymatic activities of DNase present in streptococcal supernatants and human urine. Immunization of lupus-prone NZB/NZW mice with bovine DNase enhanced the production of anti-DNase and DNA antibodies, and accelerated the occurrence of proteinuria. Taken together, these results suggest that DNase-inhibitory antibodies which recognize a conserved epitope near the catalytic site of DNase may act in the pathogenesis of SLE.
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