
GITHUB . COM {
}
Detected CMS Systems:
- Wordpress (2 occurrences)
Title:
How to handle reads with alternative adapters or primer sequences ยท Issue #744 ยท marcelm/cutadapt
Description:
Hi, It's a powerful tools for data analysis. I have a question when trimming primers from paired-end reads of amplicon. The sequencing data (e.g. forward_reads) likes: AAATTTaaaaaaaaCCCGGG and ...
Website Age:
17 years and 8 months (reg. 2007-10-09).
Matching Content Categories {๐}
- Social Networks
- Mobile Technology & AI
- Education
Content Management System {๐}
What CMS is github.com built with?
Github.com is built with WORDPRESS.
Traffic Estimate {๐}
What is the average monthly size of github.com audience?
๐๐ Tremendous Traffic: 10M - 20M visitors per month
Based on our best estimate, this website will receive around 10,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.
check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush
How Does Github.com Make Money? {๐ธ}
Subscription Packages {๐ณ}
We've located a dedicated page on github.com that might include details about subscription plans or recurring payments. We identified it based on the word pricing in one of its internal links. Below, you'll find additional estimates for its monthly recurring revenues.How Much Does Github.com Make? {๐ฐ}
Subscription Packages {๐ณ}
Prices on github.com are in US Dollars ($).
They range from $4.00/month to $21.00/month.
We estimate that the site has approximately 4,989,889 paying customers.
The estimated monthly recurring revenue (MRR) is $20,957,532.
The estimated annual recurring revenues (ARR) are $251,490,385.
Wordpress Themes and Plugins {๐จ}
What WordPress theme does this site use?
It is strange but we were not able to detect any theme on the page.
What WordPress plugins does this website use?
It is strange but we were not able to detect any plugins on the page.
Keywords {๐}
reads, revcomp, pairedend, marcelm, issue, fxfish, sign, data, commented, implement, search, cutadapt, aaattt, cccggg, rfq, swapped, version, navigation, pull, requests, actions, security, handle, alternative, adapters, primer, sequences, closed, likes, output, steps, owner, work, results, swapping, author, bbce, update, react, github, labels, projects, milestone, footer, skip, content, menu, product, solutions, resources,
Topics {โ๏ธ}
b4bc46e marcelm closed normal adapter search means recognizing โgggccc reversing complementary sequence comment metadata assignees paired-end reads single-end reads paired-end data combining 1st r1 swapped version resulted output data likes search cutadapt cutadapt 4 handle reads data analysis sequencing data swapped version unswapped version alternative adapters powerful tools trimming primers step commond functionality doesn implementing --revcomp makes sense function --revcomp assigned labels labels projects projects milestone milestone relationships personal information github 2st r2 ^cccggg --revcomp swapping r1 implement likes revcomp swapped output r1 swapping ^cccggg ^cccggg r2 skip jump sign question
Payment Methods {๐}
- Braintree
Questions {โ}
- Already have an account?
- Can I just use one step commond?
- Can you say whether that would give you the result you want?
Schema {๐บ๏ธ}
DiscussionForumPosting:
context:https://schema.org
headline:How to handle reads with alternative adapters or primer sequences
articleBody:Hi,
It's a powerful tools for data analysis.
I have a question when trimming primers from paired-end reads of amplicon. The sequencing data (e.g. forward_reads) likes: AAATTTaaaaaaaaCCCGGG and GGGCCCtttttttttTTTAAA (mixed), I want all output data likes: aaaaaaaa, which means recognizing โGGGCCC...TTTAAAโ and reversing complementary sequence.
I must do three steps to achive this, one using -g ^AAATTT -G ^CCCGGG, and another using -g ^CCCGGG -G ^AAATTT, at last combining 1st R1.fq and 2st R2.fq into the last R1.fq.
Can I just use one step commond? -g -g or -g XXX|XXX
Thanks.
<!--
When reporting an issue, please include this information:
- Cutadapt and Python version
- How you installed the tool (conda or pip, for example)
- Which command-line parameters you used
If you report unexpected trimming behavior, this would also be helpful:
- An example input read (or read pair)
- The output that cutadapt produces
- The output that you would have expected
Feel free to delete this text before submitting your issue.
-->
author:
url:https://github.com/fxfish
type:Person
name:fxfish
datePublished:2023-11-30T09:32:07.000Z
interactionStatistic:
type:InteractionCounter
interactionType:https://schema.org/CommentAction
userInteractionCount:4
url:https://github.com/744/cutadapt/issues/744
context:https://schema.org
headline:How to handle reads with alternative adapters or primer sequences
articleBody:Hi,
It's a powerful tools for data analysis.
I have a question when trimming primers from paired-end reads of amplicon. The sequencing data (e.g. forward_reads) likes: AAATTTaaaaaaaaCCCGGG and GGGCCCtttttttttTTTAAA (mixed), I want all output data likes: aaaaaaaa, which means recognizing โGGGCCC...TTTAAAโ and reversing complementary sequence.
I must do three steps to achive this, one using -g ^AAATTT -G ^CCCGGG, and another using -g ^CCCGGG -G ^AAATTT, at last combining 1st R1.fq and 2st R2.fq into the last R1.fq.
Can I just use one step commond? -g -g or -g XXX|XXX
Thanks.
<!--
When reporting an issue, please include this information:
- Cutadapt and Python version
- How you installed the tool (conda or pip, for example)
- Which command-line parameters you used
If you report unexpected trimming behavior, this would also be helpful:
- An example input read (or read pair)
- The output that cutadapt produces
- The output that you would have expected
Feel free to delete this text before submitting your issue.
-->
author:
url:https://github.com/fxfish
type:Person
name:fxfish
datePublished:2023-11-30T09:32:07.000Z
interactionStatistic:
type:InteractionCounter
interactionType:https://schema.org/CommentAction
userInteractionCount:4
url:https://github.com/744/cutadapt/issues/744
Person:
url:https://github.com/fxfish
name:fxfish
url:https://github.com/fxfish
name:fxfish
InteractionCounter:
interactionType:https://schema.org/CommentAction
userInteractionCount:4
interactionType:https://schema.org/CommentAction
userInteractionCount:4
External Links {๐}(2)
Analytics and Tracking {๐}
- Site Verification - Google
Libraries {๐}
- Clipboard.js
- D3.js
- Lodash
Emails and Hosting {โ๏ธ}
Mail Servers:
- aspmx.l.google.com
- alt1.aspmx.l.google.com
- alt2.aspmx.l.google.com
- alt3.aspmx.l.google.com
- alt4.aspmx.l.google.com
Name Servers:
- dns1.p08.nsone.net
- dns2.p08.nsone.net
- dns3.p08.nsone.net
- dns4.p08.nsone.net
- ns-1283.awsdns-32.org
- ns-1707.awsdns-21.co.uk
- ns-421.awsdns-52.com
- ns-520.awsdns-01.net