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We are analyzing https://github.com/marcelm/cutadapt/issues/394.

Title:
first adapter in linked pair is incorrectly trimmed from 5' end of read Β· Issue #394 Β· marcelm/cutadapt
Description:
Using cutadapt 2.1 compiled from source code, the below command trims the first adapter of the specified liked pair from the read in file temp1.fq. This behavior seems incorrect because many bases are mismatched near the 3
Website Age:
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Keywords {πŸ”}

adapter, trimmed, marcelm, cutadapt, linked, end, issue, noindels, reads, sign, read, behavior, pair, dmelu, tempfq, option, adapters, allowed, errors, error, cgggcaggctatgttttctttctactggatcagatcggaagagcgtcgtgtagggaaagagtgt, total, length, navigation, source, code, pull, requests, actions, security, incorrectly, closed, command, bases, correct, rate, bug, readsminute, processed, written, times, overview, removed, sequences, count, expect, maxerr, counts, commented, github,

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source code total basepairs processed marcelm closed comment metadata assignees command-line options fq processing reads cat ~/tmp/temp1 error counts 29 single-end mode reads/minute reads written 10% error rate command trims file temp1 cutadapt 2 left unchanged allowed errors passing filters removed sequences ve fixed email author assigned labels labels projects projects milestone milestone relationships personal information agatcggaagagcgtcgtgtagggaaagagtgt' temp1 agatcggaagagcgtcgtgtagggaaagagtgt temp1 observed behavior weird behavior 2 Β΅s/read incorrectly trimmed github linked pair linked adapters issue 5' end length 3' end length correct behavior trimmed adapter 1 reads fq linked behavior 5' end pair read 3' end option option

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DiscussionForumPosting:
      context:https://schema.org
      headline:first adapter in linked pair is incorrectly trimmed from 5' end of read
      articleBody:Using `cutadapt` 2.1 compiled from source code, the below command trims the first adapter of the specified liked pair from the read in file `temp1.fq`. This behavior seems incorrect because many bases are mismatched near the 3' end of the trimmed adapter. The correct behavior should be that the read is left unchanged. Also, the last two bases of the adapter are not trimmed. The observed behavior would be correct if option `--no-indels` was **not** specified. So the issue seems to be that `cutadapt` ignores option `--no-indels` when searching for linked adapters. Another weird behavior is that the numbers of allowed errors are calculate using a 10% error rate, instead of the 20% rate specified by option `-e`. ```bash cat ~/tmp/temp1.fq ``` ``` @bug CGGGCAGGCTATGTTTTCTTCTGCTGGACCCTTTCATACCTGCTTCCACTCTGACCTCCACATAAATTCCACCAGCGAGTTTCAGCTTTTTTGCCCAATTC + AAAAFJJ7JFFFJJJJJJJJJJJJJJJJJJAJFJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFJJJJFJ-FJ<AJ)A7)7FJAFFAFJJJJJFJAA<FJ< ``` ```bash cutadapt --no-indels -e 0.2 -y ' {name}' -a '3=^CGGGCAGGCTATGTTTTCTTTCTACTGGATC...AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT' temp1.fq ``` ``` This is cutadapt 2.1 with Python 3.7.2 Command line parameters: --no-indels -e 0.2 -y {name} -a 3=^CGGGCAGGCTATGTTTTCTTTCTACTGGATC...AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT temp1.fq Processing reads on 1 core in single-end mode ... [8<----------] 00:00:00 1 reads @ 1153.2 Β΅s/read; 0.05 M reads/minute @bug 3 CCTTTCATACCTGCTTCCACTCTGACCTCCACATAAATTCCACCAGCGAGTTTCAGCTTTTTTGCCCAATTC + JAJFJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFJJJJFJ-FJ<AJ)A7)7FJAFFAFJJJJJFJAA<FJ< Finished in 0.00 s (3591 us/read; 0.02 M reads/minute). === Summary === Total reads processed: 1 Reads with adapters: 1 (100.0%) Reads written (passing filters): 1 (100.0%) Total basepairs processed: 101 bp Total written (filtered): 72 bp (71.3%) === Adapter 1 === Sequence: CGGGCAGGCTATGTTTTCTTTCTACTGGATC...AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: linked; Length: 31+33; 5' trimmed: 1 times; 3' trimmed: 0 times No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-31 bp: 3 No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-33 bp: 3 Overview of removed sequences at 5' end length count expect max.err error counts 29 1 0.0 2 0 0 0 1 Overview of removed sequences at 3' end length count expect max.err error counts ```
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      datePublished:2019-08-10T01:46:18.000Z
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      url:https://github.com/394/cutadapt/issues/394
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      headline:first adapter in linked pair is incorrectly trimmed from 5' end of read
      articleBody:Using `cutadapt` 2.1 compiled from source code, the below command trims the first adapter of the specified liked pair from the read in file `temp1.fq`. This behavior seems incorrect because many bases are mismatched near the 3' end of the trimmed adapter. The correct behavior should be that the read is left unchanged. Also, the last two bases of the adapter are not trimmed. The observed behavior would be correct if option `--no-indels` was **not** specified. So the issue seems to be that `cutadapt` ignores option `--no-indels` when searching for linked adapters. Another weird behavior is that the numbers of allowed errors are calculate using a 10% error rate, instead of the 20% rate specified by option `-e`. ```bash cat ~/tmp/temp1.fq ``` ``` @bug CGGGCAGGCTATGTTTTCTTCTGCTGGACCCTTTCATACCTGCTTCCACTCTGACCTCCACATAAATTCCACCAGCGAGTTTCAGCTTTTTTGCCCAATTC + AAAAFJJ7JFFFJJJJJJJJJJJJJJJJJJAJFJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFJJJJFJ-FJ<AJ)A7)7FJAFFAFJJJJJFJAA<FJ< ``` ```bash cutadapt --no-indels -e 0.2 -y ' {name}' -a '3=^CGGGCAGGCTATGTTTTCTTTCTACTGGATC...AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT' temp1.fq ``` ``` This is cutadapt 2.1 with Python 3.7.2 Command line parameters: --no-indels -e 0.2 -y {name} -a 3=^CGGGCAGGCTATGTTTTCTTTCTACTGGATC...AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT temp1.fq Processing reads on 1 core in single-end mode ... [8<----------] 00:00:00 1 reads @ 1153.2 Β΅s/read; 0.05 M reads/minute @bug 3 CCTTTCATACCTGCTTCCACTCTGACCTCCACATAAATTCCACCAGCGAGTTTCAGCTTTTTTGCCCAATTC + JAJFJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJFJJJJFJ-FJ<AJ)A7)7FJAFFAFJJJJJFJAA<FJ< Finished in 0.00 s (3591 us/read; 0.02 M reads/minute). === Summary === Total reads processed: 1 Reads with adapters: 1 (100.0%) Reads written (passing filters): 1 (100.0%) Total basepairs processed: 101 bp Total written (filtered): 72 bp (71.3%) === Adapter 1 === Sequence: CGGGCAGGCTATGTTTTCTTTCTACTGGATC...AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT; Type: linked; Length: 31+33; 5' trimmed: 1 times; 3' trimmed: 0 times No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-31 bp: 3 No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20-29 bp: 2; 30-33 bp: 3 Overview of removed sequences at 5' end length count expect max.err error counts 29 1 0.0 2 0 0 0 1 Overview of removed sequences at 3' end length count expect max.err error counts ```
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      url:https://github.com/394/cutadapt/issues/394
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