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Prices on github.com are in US Dollars ($). They range from $4.00/month to $21.00/month.
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Keywords {🔍}

marcelm, commented, reads, owner, koopkaup, cutadapt, dual, barcodes, file, indexing, barcode, adapter, denisgoryunov, indices, sign, demultiplexing, pairedend, read, work, option, data, issue, forwardbarcodesequence, forwardprimersequence, reverseprimersequence, reversebarcode, author, fasta, combinations, index, combinatorial, unique, navigation, search, pull, requests, actions, security, primers, illumina, format, forward, reverse, linked, make, adapters, edited, edits, case, comment,

Topics {✒️}

demultiplexing paired-end reads paired-end reads untrimmed-paired-output pair-adapters option suggested dual indexing strategies unique dual indexes illumina miseq reads dual index technology /usearch/manual/pipe_demux demultiplexing file pair-adapters option provide linked adapters comment metadata assignees reverse_barcode sequences denisgoryunov edits running cutadapt cutadapt outputting search combinatorial indexing dual barcodes redundant indexing read belongs i5 index i7 index i5 indices i7 indices output files fasta file cutadapt bit inefficient dna fragment recent post forward_barcode_sequence forward_primer_sequence reverse_primer_sequence detailed discription assigned labels labels projects projects milestone milestone relationships forward barcodes reverse barcodes barcodes simultaneously random barcodes linked adapter reads read format small part sequencing facility

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Questions {❓}

• Already have an account?
• Any update regarding combinatorial indexing?
• Can you clarify whether your reads look like you describe above or whether it is the DNA fragment that you were describing?
• Can you try this and let me know whether it works?
• Could one of you send me some small part of your dataset (with just a couple of reads)?
• Have you come up with a solution for the first situation where there are multiple combinations of indices?
• I will try this, but how can I provide linked adapters as a fasta file?
• Is it possible to add an option to search for both barcodes simultaneously to decide where a read belongs to?

Schema {🗺️}

DiscussionForumPosting:
      context:https://schema.org
      headline:Demultiplexing paired-end reads with dual barcodes and primers
      articleBody:Hi, I have Illumina MiSeq reads in the following format forward_barcode_sequence forward_primer_sequence read reverse_primer_sequence reverse_barcode Because forward barcodes is used in combination with reverse barcodes, searching only the first barcode as cutadapt does right now does not work. Is it possible to add an option to search for both barcodes simultaneously to decide where a read belongs to?
      author:
         url:https://github.com/koopkaup
         type:Person
         name:koopkaup
      datePublished:2018-02-21T10:28:17.000Z
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      url:https://github.com/292/cutadapt/issues/292
      context:https://schema.org
      headline:Demultiplexing paired-end reads with dual barcodes and primers
      articleBody:Hi, I have Illumina MiSeq reads in the following format forward_barcode_sequence forward_primer_sequence read reverse_primer_sequence reverse_barcode Because forward barcodes is used in combination with reverse barcodes, searching only the first barcode as cutadapt does right now does not work. Is it possible to add an option to search for both barcodes simultaneously to decide where a read belongs to?
      author:
         url:https://github.com/koopkaup
         type:Person
         name:koopkaup
      datePublished:2018-02-21T10:28:17.000Z
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      url:https://github.com/292/cutadapt/issues/292
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      url:https://github.com/koopkaup
      name:koopkaup
      url:https://github.com/koopkaup
      name:koopkaup
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• Check the income stats for https://www.drive5.com/usearch/manual/pipe_demux.html
• Financial intake of https://support.illumina.com/bulletins/2018/08/understanding-unique-dual-indexes--udi--and-associated-library-p.html
• How much cash flow does https://www.githubstatus.com/ have monthly?

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