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We began analyzing https://elifesciences.org/articles/03346, but it redirected us to https://elifesciences.org/articles/03346. The analysis below is for the second page.

Title[redir]:
Identification of the transcription factor ZEB1 as a central component of the adipogenic gene regulatory network | eLife
Description:
The growing rates of obesity and related metabolic diseases are a major public health concern worldwide. People who are overweight or obese are at increased risk for a range of diseases including diabetes and heart disease, which may reduce their quality of life and shorten their lifespans. Obese people have more, larger fat cells than individuals of healthy weight, and so understanding how the body creates fat cells may provide new insights into ways to prevent or treat obesity. Fat cells arise from a population of stem cells that can also give rise to bone cells and cartilage. Some of the proteins—called transcription factors—that work together to turn on the expression of genes needed for a stem cell to become a fat cell have been identified. However, the exact regulatory processes that cause an unspecialized cell to develop into a fat cell remain unclear. Gubelmann et al. set out to identify more of the transcription factors that cause stem cells to become fat cells. A high-throughput, automated process was used to test the effect of over-expressing each of 734 transcription factors in mouse cells that are primed to become fat cells. Twenty-six transcription factors were found to increase the number of these primed cells that became mature fat cells—most of which had not previously been shown to affect how fat cells develop. The most powerful driver of fat cell development was ZEB1: a transcription factor that has previously been implicated in many other biological processes. Most notably, ZEB1 was linked to a transition during development that allows cells to migrate to the correct location in the embryo, but also to a mechanism that allows cancerous cells to spread to new tissues. Using studies of mouse cells and live mice, computational analyses, and biopsies from obese patients, Gubelmann et al. show that ZEB1 regulates numerous other transcription factors that promote the development of fat cells. These include factors that initially set an unspecialized cell on the path to becoming a fat cell and those that guide the changes that occur as the fat cell matures. Further studies will be required to show whether the ZEB1 protein itself is needed to prime stem cells to start becoming fat cells.

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Keywords {🔍}

zeb, figure, cells, differentiation, expression, google, scholar, supplement, adipogenesis, cell, data, adipogenic, day, regions, fat, genes, gene, cebpβ, binding, tfs, gubelmann, levels, regulatory, analysis, regulators, control, adipocyte, transcription, supplementary, file, protocol, previously, asset, dna, factors, competing, interests, life, bound, performed, mouse, request, detailed, open, preadipocytes, days, significantly, lausanne, found, molecular,

Topics {✒️}

heid 15-cm length 15 cm encode [gerstein plenti6/ubc/v5-dest expression vector gov/geo/ myers rpauli lens-specific δ1-crystalline enhancer carine gubelmann sebastian wild-type 3t3-l1 cells including ‘tgf-beta-dependent induction bart deplancke institute adipogenesis [pérez-mancera multiple-testing corrected p-values wilcoxon rank-sum test kilpinen lc-ms/ms injections large-scale transcription factor large-scale overexpression screen sided p-values corrected trizol/chlorophorm extraction procedure fast gapped-read alignment chip-seq library preparation quantitative real-time pcr super-enhancers id gse56872 control 3t3-l1 pre-adipocytes open/active regulatory regions encode/haib id gse32465 zfp277-ha overexpressing cells high-throughput screening illustrating high-throughput screening approaches rustici phosphate-buffered saline 1x ms/ms scans acquired transduce 3t3-l1 cells sheep anti-rabbit igg add1/srebp1 activates ppargamma gateway-compactible expression vectors human chip-seq datasets open access database uk/arrayexpress transcript-specific primer database arrayexpress update—trends correspondence bart cytoskeleton remodeling-related processes transcription factor-binding profiles microarray-based gene expression statistical threshold-independent manner adipogenic-specific regulatory regions chromatin state-based inference full-ms scans acquired

Schema {🗺️}

ScholarlyArticle:
      context:https://schema.org
      mainEntityOfPage:
         type:WebPage
         id:https://elifesciences.org/articles/03346
      headline:Identification of the transcription factor ZEB1 as a central component of the adipogenic gene regulatory network
      datePublished:2014-08-27
      author:
            type:Person
            name:Carine Gubelmann
            type:Person
            name:Petra C Schwalie
            type:Person
            name:Sunil K Raghav
            type:Person
            name:Eva Röder
            type:Person
            name:Tenagne Delessa
            type:Person
            name:Elke Kiehlmann
            type:Person
            name:Sebastian M Waszak
            type:Person
            name:Andrea Corsinotti
            type:Person
            name:Gilles Udin
            type:Person
            name:Wiebke Holcombe
            type:Person
            name:Gottfried Rudofsky
            type:Person
            name:Didier Trono
            type:Person
            name:Christian Wolfrum
            type:Person
            name:Bart Deplancke
      publisher:
         type:Organization
         name:eLife Sciences Publications, Ltd
         logo:
            type:ImageObject
            url:https://elifesciences.org/assets/patterns/img/patterns/organisms/[email protected]
      keywords:
         adipogenesis
         zeb1
         transcription factor screen
         gene regulatory network
         mesenchymal stem cell
      about:
         Developmental Biology
      description:A large-scale transcription factor screen reveals over twenty novel adipogenic regulators: most notably ZEB1, which exerts essential transcriptional control of fat cell differentiation.
      isPartOf:
         type:Periodical
         name:eLife
         issn:2050-084X
WebPage:
      id:https://elifesciences.org/articles/03346
Person:
      name:Carine Gubelmann
      name:Petra C Schwalie
      name:Sunil K Raghav
      name:Eva Röder
      name:Tenagne Delessa
      name:Elke Kiehlmann
      name:Sebastian M Waszak
      name:Andrea Corsinotti
      name:Gilles Udin
      name:Wiebke Holcombe
      name:Gottfried Rudofsky
      name:Didier Trono
      name:Christian Wolfrum
      name:Bart Deplancke
Organization:
      name:eLife Sciences Publications, Ltd
      logo:
         type:ImageObject
         url:https://elifesciences.org/assets/patterns/img/patterns/organisms/[email protected]
ImageObject:
      url:https://elifesciences.org/assets/patterns/img/patterns/organisms/[email protected]
Periodical:
      name:eLife
      issn:2050-084X

Social Networks {👍}(10)

External Links {🔗}(1266)

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