Here's how DOI.ORG makes money* and how much!

*Please read our disclaimer before using our estimates.
Loading...

DOI . ORG {}

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Doi.org Make Money
  6. Keywords
  7. Topics
  8. Questions
  9. Schema
  10. Social Networks
  11. External Links
  12. Analytics And Tracking
  13. Libraries
  14. Hosting Providers
  15. CDN Services

We began analyzing https://www.tandfonline.com/doi/abs/10.4161/rna.26214, but it redirected us to https://www.tandfonline.com/doi/abs/10.4161/rna.26214. The analysis below is for the second page.

Title[redir]:
Full article: 4-thiouridine inhibits rRNA synthesis and causes a nucleolar stress response
Description:
High concentrations (> 100 µM) of the ribonucleoside analog 4-thiouridine (4sU) is widely used in methods for RNA analysis like photoactivatable-ribonucleoside-enhanced crosslinking and immunopreci...

Matching Content Categories {📚}

  • Science
  • Education
  • Dating & Relationships

Content Management System {📝}

What CMS is doi.org built with?

Custom-built

No common CMS systems were detected on Doi.org, and no known web development framework was identified.

Traffic Estimate {📈}

What is the average monthly size of doi.org audience?

🌠 Phenomenal Traffic: 5M - 10M visitors per month


Based on our best estimate, this website will receive around 5,000,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.

check SE Ranking
check Ahrefs
check Similarweb
check Ubersuggest
check Semrush

How Does Doi.org Make Money? {💸}

We're unsure how the site profits.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Doi.org could have a money-making trick up its sleeve, but it's undetectable for now.

Keywords {🔍}

open, window, rrna, rna, processing, cells, citation, google, scholar, pubmed, pmid, web, science, cell, synthesis, labeling, nucleolar, nascent, analysis, production, treatment, inhibition, total, fig, uridine, uos, signals, min, concentrations, inhibits, stress, proliferation, metabolism, sutagging, ribosomal, npm, ets, levels, treated, porthophosphate, control, protein, biol, show, high, experiments, ribosome, analyzed, degradation, gel,

Topics {✒️}

streptavidin-coated magnetic beads [32p]-ortho-phosphate metabolic labeling 4su-biotin-streptavidin-tagged rna google scholar hernandez-verdun photoactivatable-ribonucleoside-enhanced crosslinking additional material disclosure comparable time-dependent increase mdm2-mediated p53 ubiquitination receive personalised research francis group 4su-tagged rna shows twitter page taylor vivo double-labeling workflow cy3-labeled secondary antibody cellular rna-binding proteins 4su-mediated nucleolar stress 32p-labeled nascent rrna long-range secondary structures [32p]-ortho-phosphate pre-ribosomal rna processing gene expression profiling tumor suppressor p53 p53 tumor suppressor short-term 4su exposure rna-binding protein aberrant 28s rrna google scholar hafner google scholar schwanhäusser google scholar rabani google scholar fatica google scholar könig google scholar kurki google scholar boulon google scholar kenzelmann google scholar srivastava google scholar ghoshal google scholar zhao google scholar jack google scholar ljungman remaining primary transcript methods tissue culture aberrant rrna forms limited influence phosphate-buffered saline rna prevents pseudouridylation internal transcribed spacer-2 google scholar burger μmacs washing buffer google scholar windhager dose-dependent translocation

Questions {❓}

  • 22 How can this difference be explained?
  • How can the inhibition of rRNA synthesis by 4sU be explained on the molecular level?

Schema {🗺️}

BreadcrumbList:
      context:https://schema.org
      itemListElement:
            type:ListItem
            position:1
            name:Home
            item:https://www.tandfonline.com/
            type:ListItem
            position:2
            name:All Journals
            item:https://www.tandfonline.com/action/showPublications?pubType=journal
            type:ListItem
            position:3
            name:Bioscience
            item:https://www.tandfonline.com/subjects/bioscience
            type:ListItem
            position:4
            name:RNA Biology
            item:https://www.tandfonline.com/krnb20
            type:ListItem
            position:5
            name:List of Issues
            item:https://www.tandfonline.com/loi/krnb20
            type:ListItem
            position:6
            name:Volume 10, Issue 10
            item:https://www.tandfonline.com/toc/krnb20/10/10
            type:ListItem
            position:7
            name:4-thiouridine inhibits rRNA synthesis an ....
ListItem:
      position:1
      name:Home
      item:https://www.tandfonline.com/
      position:2
      name:All Journals
      item:https://www.tandfonline.com/action/showPublications?pubType=journal
      position:3
      name:Bioscience
      item:https://www.tandfonline.com/subjects/bioscience
      position:4
      name:RNA Biology
      item:https://www.tandfonline.com/krnb20
      position:5
      name:List of Issues
      item:https://www.tandfonline.com/loi/krnb20
      position:6
      name:Volume 10, Issue 10
      item:https://www.tandfonline.com/toc/krnb20/10/10
      position:7
      name:4-thiouridine inhibits rRNA synthesis an ....
PublicationIssue:
      id:#issue
      issueNumber:10
      datePublished:2013-10-01
      isPartOf:
         id:#periodical
         type:
            PublicationVolume
            Periodical
         name:RNA Biology
         issn:
            1547-6286
            1555-8584
         volumeNumber:10
         publisher:Taylor & Francis Group
["PublicationVolume","Periodical"]:
      id:#periodical
      name:RNA Biology
      issn:
         1547-6286
         1555-8584
      volumeNumber:10
      publisher:Taylor & Francis Group
ScholarlyArticle:
      mainEntityOfPage:https://www.tandfonline.com/doi/full/10.4161/rna.26214
      url:https://www.tandfonline.com/doi/full/10.4161/rna.26214
      isPartOf:#periodical
      sameAs:https://doi.org/10.4161/rna.26214
      identifier:10.4161/rna.26214
      isAccessibleForFree:false
      articleSection:Research Paper
      name:4-thiouridine inhibits rRNA synthesis and causes a nucleolar stress response
      headline:4-thiouridine inhibits rRNA synthesis and causes a nucleolar stress response
      abstract:High concentrations (> 100 µM) of the ribonucleoside analog 4-thiouridine (4sU) is widely used in methods for RNA analysis like photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) and nascent messenger (m)RNA labeling (4sU-tagging). Here, we show that 4sU-tagging at low concentrations ≤ 10 µM can be used to measure production and processing of ribosomal (r)RNA. However, elevated concentrations of 4sU (> 50 µM), which are usually used for mRNA labeling experiments, inhibit production and processing of 47S rRNA. The inhibition of rRNA synthesis is accompanied by nucleoplasmic translocation of nucleolar nucleophosmin (NPM1), induction of the tumor suppressor p53, and inhibition of proliferation. We conclude that metabolic labeling of RNA by 4sU triggers a nucleolar stress response, which might influence the interpretation of results. Therefore, functional ribosome biogenesis, nucleolar integrity, and cell cycle should be addressed in 4sU labeling experiments.
      description:High concentrations (> 100 µM) of the ribonucleoside analog 4-thiouridine (4sU) is widely used in methods for RNA analysis like photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) and nascent messenger (m)RNA labeling (4sU-tagging). Here, we show that 4sU-tagging at low concentrations ≤ 10 µM can be used to measure production and processing of ribosomal (r)RNA. However, elevated concentrations of 4sU (> 50 µM), which are usually used for mRNA labeling experiments, inhibit production and processing of 47S rRNA. The inhibition of rRNA synthesis is accompanied by nucleoplasmic translocation of nucleolar nucleophosmin (NPM1), induction of the tumor suppressor p53, and inhibition of proliferation. We conclude that metabolic labeling of RNA by 4sU triggers a nucleolar stress response, which might influence the interpretation of results. Therefore, functional ribosome biogenesis, nucleolar integrity, and cell cycle should be addressed in 4sU labeling experiments.
      author:
            type:Person
            name:Dirk Eick
            type:Person
            name:Kaspar Burger
            type:Person
            name:Markus Kellner
            type:Person
            name:Caroline C Friedel
            type:Person
            name:Lars Dölken
            type:Person
            name:Michaela Rohrmoser
            type:Person
            name:Bastian Mühl
            type:Person
            name:Lukas Windhager
            type:Person
            name:Anita Gruber-Eber
            type:Person
            name:Dirk Eick
            type:Person
            name:Dirk Eick
      keywords:4-thiouridine, ribosomal RNA, rRNA processing, p53, nucleolar stress, RNA labeling, nucleophosmin
      pageStart:1623
      pageEnd:1630
      datePublished:2013-09-04
      publisher:
         type:Organization
         name:Taylor & Francis
         logo:
            type:ImageObject
            url:https://www.tandfonline.com/pb-assets/Images/Taylor_and_Francis_Group_Logo-1742461082.png
Person:
      name:Dirk Eick
      name:Kaspar Burger
      name:Markus Kellner
      name:Caroline C Friedel
      name:Lars Dölken
      name:Michaela Rohrmoser
      name:Bastian Mühl
      name:Lukas Windhager
      name:Anita Gruber-Eber
      name:Dirk Eick
      name:Dirk Eick
Organization:
      name:Taylor & Francis
      logo:
         type:ImageObject
         url:https://www.tandfonline.com/pb-assets/Images/Taylor_and_Francis_Group_Logo-1742461082.png
ImageObject:
      url:https://www.tandfonline.com/pb-assets/Images/Taylor_and_Francis_Group_Logo-1742461082.png

Social Networks {👍}(4)

External Links {🔗}(226)

Analytics and Tracking {📊}

  • Google Analytics
  • Google Analytics 4
  • Google Tag Manager
  • Google Universal Analytics
  • Hotjar

Libraries {📚}

  • Dropzone.js
  • jQuery

Emails and Hosting {✉️}

Mail Servers:

  • mx.zoho.eu
  • mx2.zoho.eu
  • mx3.zoho.eu

Name Servers:

  • josh.ns.cloudflare.com
  • zita.ns.cloudflare.com

CDN Services {📦}

  • Cookielaw
  • Optimizely
  • Pbgrd

3.26s.