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Title[redir]:
Full article: Can AtTZF1 act as a transcriptional activator or repressor in plants?
Description:
The effects of AtTZF1 (At2g25900) on plant growth, development and abiotic stress response have been described recently in reference 1. In this report, differential expression of several clusters o...
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Keywords {π}
open, window, attzf, citation, science, transcriptional, activation, web, google, scholar, pubmed, transcription, yeast, protein, plant, zinc, finger, luciferase, nucleus, expression, assays, reporter, activity, system, fig, control, journals, arabidopsis, dna, transactivation, maize, domain, taylor, tandem, gene, results, nuclear, assay, oslic, galdbd, francis, factor, binding, eelr, galdbdattzf, ccch, tzf, proteins, growth, localization,
Topics {βοΈ}
gfp-gal4dbd-attzf1 fusion protein delineate critical regions/residues sip1/sip2/gal83 component full length camv35s-gal4bs de-stabilizing target mrnas single zinc finger gfp-gal4dbd-attzf1 protein twitter page taylor firefly/renilla luciferase activities r-dependent activity gfp-gal4dbd-attzf1 showed gal4 transactivation protein maize protoplast system inactivates crm1/exportin 1 francis group journal search calls ga-mediated growth gal4dbd-attzf1 fusion constructs positive control gal4dbd-snf1 arabidopsis brassinosteroid signaling negative control gfp-attzf1 zinc finger transcription factors tested gal4ad-snf4 activated transcription nucleocytoplasmic shuttling ccch tzf tis11d vivo dna/rna targets transcription factor activity native arabidopsis system gal4 binding site eelr activation domain gal4 activation domain google scholar wang google scholar carballo google scholar murata google scholar ghosh google scholar tenenhaus google scholar jiang google scholar kudo google scholar ryu snf1 protein kinase transcriptional activation activities ohio agricultural research references lin pc receive personalised research oslic transcription factor gfp-gal4dbd-attzf1 journals browse transcriptional activation activity tzf family characterized
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datePublished:2011-05-01
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articleSection:Addendum
name:Can AtTZF1 act as a transcriptional activator or repressor in plants?
headline:Can AtTZF1 act as a transcriptional activator or repressor in plants?
abstract:In animals, Tandem CCCH Zinc Finger (TZF) proteins can affect gene expression at both transcriptional and post-transcriptional levels. In Arabidopsis thaliana, AtTZF1 is a member of the TZF family characterized by a plant-unique tandem zinc finger motif. AtTZF1 can bind both DNA and RNA in vitro, and it can traffic between the nucleus and cytoplasmic foci. However, no in vivo DNA/RNA targets have been identified so far, and little is known about the molecular mechanisms underlying AtTZF1βs profound effects on plant growth, development, and stress responses. In order to determine whether AtTZF1 can function as a transcription factor, transactivation assays were conducted. Results indicated that AtTZF1 fusion proteins could not exert obvious transcriptional activity in a maize protoplast transient expression system. However, this conclusion might be biased due to poor nuclear localization of AtTZF1 fusion proteins in the assay system.
description:In animals, Tandem CCCH Zinc Finger (TZF) proteins can affect gene expression at both transcriptional and post-transcriptional levels. In Arabidopsis thaliana, AtTZF1 is a member of the TZF family characterized by a plant-unique tandem zinc finger motif. AtTZF1 can bind both DNA and RNA in vitro, and it can traffic between the nucleus and cytoplasmic foci. However, no in vivo DNA/RNA targets have been identified so far, and little is known about the molecular mechanisms underlying AtTZF1βs profound effects on plant growth, development, and stress responses. In order to determine whether AtTZF1 can function as a transcription factor, transactivation assays were conducted. Results indicated that AtTZF1 fusion proteins could not exert obvious transcriptional activity in a maize protoplast transient expression system. However, this conclusion might be biased due to poor nuclear localization of AtTZF1 fusion proteins in the assay system.
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name:Marcelo Pomeranz
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