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We began analyzing https://www.oncotarget.com/article/8431/text/, but it redirected us to https://www.oncotarget.com/article/8431/text/. The analysis below is for the second page.

Title[redir]:
Mutational analysis of single circulating tumor cells by next generation sequencing in metastatic breast cancer | Oncotarget
Description:
// Francesca De Luca 1, * , Giada Rotunno 2, * , Francesca Salvianti 3, * , Francesca Galardi 1 , Marta Pestrin 1 , Stefano Gabellini 1 , Lisa Simi 3 , Irene Mancini 3 , Alessandro Maria Vannucchi 2 , Mario Pazzagli 3 , Angelo Di Leo 1 , Pamela Pinzani 3 1 Sandro Pitigliani Medical Oncology Department, Hospital of Prato, Istituto Toscano Tumori, 59100, Prato, Italy 2 Department of Experimental and Clinical Medicine, University of Florence, 50139, Florence, Italy 3 Department of Clinical, Experimental and Biomedical Sciences, University of Florence, 50139, Florence, Italy * These authors contributed equally to this work Correspondence to: Pamela Pinzani, email: [email protected] Keywords: breast cancer, circulating tumor cells, next generation sequencing, single cell sequencing, somatic mutations Received: December 21, 2015      Accepted: March 07, 2016      Published: March 28, 2016 ABSTRACT Circulating Tumor Cells (CTCs) represent a “liquid biopsy” of the tumor potentially allowing real-time monitoring of cancer biology and therapies in individual patients. The purpose of the study was to explore the applicability of a protocol for the molecular characterization of single CTCs by Next Generation Sequencing (NGS) in order to investigate cell heterogeneity and provide a tool for a personalized medicine approach. CTCs were enriched and enumerated by CellSearch in blood from four metastatic breast cancer patients and singularly isolated by DEPArray. Upon whole genome amplification 3–5 single CTCs per patient were analyzed by NGS for 50 cancer-related genes. We found 51 sequence variants in 25 genes. We observed inter- and intra-patient heterogeneity in the mutational status of CTCs. The highest number of somatic deleterious mutations was found in the gene TP53 , whose mutation is associated with adverse prognosis in breast cancer. The discordance between the mutational status of the primary tumor and CTCs observed in 3 patients suggests that, in advanced stages of cancer, CTC characteristics are more closely linked to the dynamic modifications of the disease status. In one patient the mutational profiles of CTCs before and during treatment shared only few sequence variants. This study supports the applicability of a non-invasive approach based on the liquid biopsy in metastatic breast cancer patients which, in perspective, should allow investigating the clonal evolution of the tumor for the development of new therapeutic strategies in precision medicine.

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Keywords {🔍}

cancer, ctcs, single, cells, tumor, breast, patients, sequencing, variants, gene, mutations, cell, ctc, circulating, analysis, patient, metastatic, blood, number, sequence, samples, ion, exon, biopsy, genes, clinical, study, ngs, heterogeneity, cellsearch, kit, treatment, molecular, characterization, status, generation, liquid, approach, genome, found, detected, results, system, sample, dna, oncotarget, mutation, primary, disease, tissue,

Topics {✒️}

formalin-fixed paraffin embedded formalin-fixed paraffin-embedded current interventional trials pi3k/akt/mtor pathway [37] single-cell sequencing-based technologies fluorescence-activated cell sorting genomic high-resolution profiling inaugural editorial indirect pcr-based methods publication alerts subscribe open-access journal dedicated free publication ion ampliseq™ dna future medical applications ion torrent pgm real-time monitoring post-therapy condition single cell sorting single-cell sorting celltracks® analyzer ii comparative genomic hybridization ion torrent chemistry metastatic breast cancer metastatic breast cancer analyze nucleic acids homopolymer-based errors ferrofluids particles coated staining pattern deriving circulating tumor cells tumor cells circulating progression-free survival natl cancer inst angelo di leo1 circulating melanoma cells circulating tumour cells tcr repertoire profiling single cell resolution invasive approach based automated fluorescence microscope dielectrophoresis-based device ck19 positive cells positive fluorescent signal breast cancer res maximize research impact metastatic site biopsy persisting tumor cells cd45 negative cells single–cell technology platform-specific features cancer metastasis rev

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Do circulating tumor cells, exosomes, and circulating tumor nucleic acids have clinical utility?

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Article:
      license:https://creativecommons.org/licenses/by/4.0/
      publisher:
         type:Organization
         name:Oncotarget
         logo:
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      dateModified:2016-06-08T09:54:06Z
      mainEntityOfPage:
         type:WebPage
         id:https://www.oncotarget.com/article/8431/text/
      headline:Mutational analysis of single circulating tumor cells by next generation sequencing in metastatic breast ca
      articleSection:Research Papers
      sameAs:https://doi.org/10.18632/oncotarget.8431
      image:https://www.oncotarget.com/index.php?journal=oncotarget&page=article&op=viewFile&path%5B%5D=8431&path%5B%5D=25032&path%5B%5D=128020
      datePublished:2016-03-28T00:00:00Z
      author:
            type:Person
            name:Luca, Francesca De
            type:Person
            name:Rotunno, Giada
            type:Person
            name:Salvianti, Francesca
            type:Person
            name:Galardi, Francesca
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            name:Gabellini, Stefano
            type:Person
            name:Simi, Lisa
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            name:Vannucchi, Alessandro Maria
            type:Person
            name:Pazzagli, Mario
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            name:Leo, Angelo Di
            type:Person
            name:Pinzani, Pamela
Organization:
      name:Oncotarget
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         url:https://www.oncotarget.com/images/oncotarget-logo-square.png
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WebPage:
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Person:
      name:Luca, Francesca De
      name:Rotunno, Giada
      name:Salvianti, Francesca
      name:Galardi, Francesca
      name:Pestrin, Marta
      name:Gabellini, Stefano
      name:Simi, Lisa
      name:Mancini, Irene
      name:Vannucchi, Alessandro Maria
      name:Pazzagli, Mario
      name:Leo, Angelo Di
      name:Pinzani, Pamela

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