DOI . ORG {
}
Detected CMS Systems:
- Apache Lenya (2 occurrences)
- Wordpress (1 occurrences)
- Hubspot (1 occurrences)
We began analyzing https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1005473, but it redirected us to https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1005473. The analysis below is for the second page.
Title[redir]:
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Description:
Author Summary Ribosome profiling is emerging as a powerful technique to monitor translation in living cells at sub-codon resolution. It has particular applicability to virology, with the capacity to identify viral mRNAs that are being translated during infection and to provide new insights into virus gene expression, regulation and host-virus interactions. In this work, we carried out the first ribosome profiling analysis of an RNA virus, using as a model system the murine coronavirus strain MHV-A59, a betacoronavirus in the same genus as the medically important SARS-CoV and MERS-CoV. Parallel ribosome profiling and RNA sequencing of infected-cell time points was performed during the course of MHV replication in mouse tissue culture cells and used to determine virus gene expression kinetics and the relative translational efficiencies of virus and host mRNAs. The sensitivity and precision of the approach permitted us to uncover several unanticipated features of coronavirus translation, giving insights into ribosomal frameshifting, ribosome pausing, and the utilisation of short, potentially regulatory, upstream open reading frames. We also identified some challenges associated with the technique that are of general relevance to the ribosome profiling technique and developed bioinformatic strategies to address these.
Title[redir]:
No title found...
Description:
Author Summary Ribosome profiling is emerging as a powerful technique to monitor translation in living cells at sub-codon resolution. It has particular applicability to virology, with the capacity to identify viral mRNAs that are being translated during infection and to provide new insights into virus gene expression, regulation and host-virus interactions. In this work, we carried out the first ribosome profiling analysis of an RNA virus, using as a model system the murine coronavirus strain MHV-A59, a betacoronavirus in the same genus as the medically important SARS-CoV and MERS-CoV. Parallel ribosome profiling and RNA sequencing of infected-cell time points was performed during the course of MHV replication in mouse tissue culture cells and used to determine virus gene expression kinetics and the relative translational efficiencies of virus and host mRNAs. The sensitivity and precision of the approach permitted us to uncover several unanticipated features of coronavirus translation, giving insights into ribosomal frameshifting, ribosome pausing, and the utilisation of short, potentially regulatory, upstream open reading frames. We also identified some challenges associated with the technique that are of general relevance to the ribosome profiling technique and developed bioinformatic strategies to address these.
Matching Content Categories {π}
- Education
- Science
- Video & Online Content
Content Management System {π}
What CMS is doi.org built with?
Doi.org is powered by APACHE LENYA.
But there are also traces of other content systems on the page (wordpress, 1hubspot).
Traffic Estimate {π}
What is the average monthly size of doi.org audience?
π Impressive Traffic: 500k - 1M visitors per month
Based on our best estimate, this website will receive around 600,019 visitors per month in the current month.
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How Does Doi.org Make Money? {πΈ}
We can't tell how the site generates income.
The purpose of some websites isn't monetary gain; they're meant to inform, educate, or foster collaboration. Everyone has unique reasons for building websites. This could be an example. Doi.org might have a hidden revenue stream, but it's not something we can detect.
Keywords {π}
fig, virus, mrna, reads, rna, view, article, translation, google, scholar, pmid, pubmedncbi, rnaseq, riboseq, orf, host, codon, ribosome, coronavirus, initiation, mrnas, ribosomes, repeat, density, rpfs, length, time, ribosomal, profiling, orfa, mhv, sequence, protein, frameshifting, expression, region, upstream, pausing, genome, cells, read, samples, data, orfs, distributions, chx, mapping, analysis, codons, positions,
Topics {βοΈ}
health molecular biology messenger rna computational analysis plos biol 3 plos biol 6 plos biol 11 plos genet 11 beijing genomics institute plos pathog 12 plos pathog 11 plos pathog 10 cookies plos medicine full-length cdna replicates contributed reagents/materials/analysis tools mammalian systems [8] digested xhoi/pvuii-pps0 plasmid vitro systems coronavirus replication complex irdye-conjugated secondary antibody replication complex formation cdna fragment comprising protein a-sepharose cl-4b inserted cdna encoding bamh1-xhoi-digested pcr fragments poly-adp-ribose-binding activity user-friendly software package laboratory-adapted strain mhv-a59 3β² untranslated regions 3' untranslated regions dual-luciferase reporter system original author negative-sense rna plateauing global βsnap-shotβ subgenomic negative-sense templates account global shut larger gel slice positive-sense riboseq/rnaseq ratio post-termination 40s subunits leader/body transcriptional discontinuity macromolecular complex [47 junction/body chimeric reads cdna derived virus protein-coding regions negative-sense virus rna nucleic-acid binding domain 50 ΞΌg/ml deae-dextran virus positive-sense rna positive-sense virus rna
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