DOI . ORG {
}
Detected CMS Systems:
- Apache Lenya (8 occurrences)
- Wordpress (1 occurrences)
- Hubspot (1 occurrences)
We began analyzing https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003565, but it redirected us to https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003565. The analysis below is for the second page.
Title[redir]:
No title found...
Description:
Author Summary RNA viruses have high mutation rates (10โ3 to 10โ5 mutations/nucleotide/round of replication), allowing for rapid viral adaptation in response to selective pressure. While RNA viruses have long been considered unable to correct mistakes during replication, CoVs such as SARS-CoV and the recently emerged MERS-CoV are important exceptions to this paradigm. All CoVs encode an exoribonuclease activity in nonstructural protein 14 (nsp14-ExoN) that is proposed to prevent and/or remove misincorporated nucleotides. Because of the demonstrated resistance of SARS-CoV to the antiviral drug ribavirin (RBV), we hypothesized that ExoN is responsible for CoV resistance to RNA mutagens. Using RBV and the RNA mutagen 5-fluorouracil (5-FU), we show that CoVs lacking ExoN activity (ExoNโ) are highly susceptible to RBV and 5-FU, in contrast to wild-type (ExoN+) CoVs. The inhibitory activity of 5-FU against ExoNโ viruses resulted specifically from 5-FU incorporation during viral RNA synthesis that lead to extensive mutagenesis within the viral population, and was associated with a profound decrease in virus specific infectivity. These results demonstrate the proofreading activity of ExoN during virus replication and suggest that inhibitors of ExoN activity could be broadly useful inhibitors of CoV replication in combination with RBV or RNA mutagens.
Title[redir]:
No title found...
Description:
Author Summary RNA viruses have high mutation rates (10โ3 to 10โ5 mutations/nucleotide/round of replication), allowing for rapid viral adaptation in response to selective pressure. While RNA viruses have long been considered unable to correct mistakes during replication, CoVs such as SARS-CoV and the recently emerged MERS-CoV are important exceptions to this paradigm. All CoVs encode an exoribonuclease activity in nonstructural protein 14 (nsp14-ExoN) that is proposed to prevent and/or remove misincorporated nucleotides. Because of the demonstrated resistance of SARS-CoV to the antiviral drug ribavirin (RBV), we hypothesized that ExoN is responsible for CoV resistance to RNA mutagens. Using RBV and the RNA mutagen 5-fluorouracil (5-FU), we show that CoVs lacking ExoN activity (ExoNโ) are highly susceptible to RBV and 5-FU, in contrast to wild-type (ExoN+) CoVs. The inhibitory activity of 5-FU against ExoNโ viruses resulted specifically from 5-FU incorporation during viral RNA synthesis that lead to extensive mutagenesis within the viral population, and was associated with a profound decrease in virus specific infectivity. These results demonstrate the proofreading activity of ExoN during virus replication and suggest that inhibitors of ExoN activity could be broadly useful inhibitors of CoV replication in combination with RBV or RNA mutagens.
Matching Content Categories {๐}
- Science
- Education
- Books & Literature
Content Management System {๐}
What CMS is doi.org built with?
Doi.org employs APACHE LENYA.
But there are also traces of other content systems on the page (wordpress, 1hubspot).
Traffic Estimate {๐}
What is the average monthly size of doi.org audience?
๐ Impressive Traffic: 500k - 1M visitors per month
Based on our best estimate, this website will receive around 600,019 visitors per month in the current month.
However, some sources were not loaded, we suggest to reload the page to get complete results.
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How Does Doi.org Make Money? {๐ธ}
We can't see how the site brings in money.
Websites don't always need to be profitable; some serve as platforms for education or personal expression. Websites can serve multiple purposes. And this might be one of them. Doi.org has a secret sauce for making money, but we can't detect it yet.
Keywords {๐}
exon, view, article, google, scholar, rna, rbv, virus, viruses, replication, mhvexon, activity, treatment, shown, cov, viral, sarsexon, cells, figure, sarscov, fold, mutagenesis, plos, sensitivity, antiviral, mutations, fidelity, transitions, proofreading, ribavirin, covs, determined, lethal, coronavirus, inhibition, studies, values, increased, polymerase, dmem, genomic, respiratory, synthesis, untreated, population, dna, severe, biol, evidence, human,
Topics {โ๏ธ}
global health health grants u54-ai057157 plos pathogens staff step real-time qrt-pcr mhv-a59 replicase protein-nsp10 bacterially-expressed sars-cov nsp14-exon medicine plos pathogens 10 plos pathog 9 plos pathog 6 plos pathog 8 plos pathog 1 real-time quantitative pcr plos med 3 cookies plos real-time qrt-pcr open-access article distributed chikungunya viruses [48]โ[50] plos reverse genetics rna-dependent rna polymerase high mutation rates emerging human coronavirus-emc chikungunya high-fidelity polymerase easy broad-spectrum antiviral agent burrows-wheeler alignment tool original author full-length infectious cdna controlled double-blind study cov trans-species movement drive high-level mutagenesis mers-covโcausing colds recently emerged mers-cov sequence alignment/map format broad-spectrum antiviral activity phylogenetically distant ฮฒ-covs inhibit sars-cov replication mutagenesis-mediated virus extinction coronaviruses post-sars conserved d-e-d coronavirus rna synthesis mhv-exonโ genomic rna potential pan-cov therapeutics single mutation viral protein distinct mhv-exon+ rna levels g641d mutation inhibit mhv-exonโ replication results mhv-exonโ viruses
Questions {โ}
- Crotty S, Cameron C, Andino R (2002) Ribavirin's antiviral mechanism of action: lethal mutagenesis?
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Analytics and Tracking {๐}
- Facebook Pixel
- Google Analytics
- Google Analytics 4
- Google Tag Manager
- Google Universal Analytics
- HubSpot
Libraries {๐}
- Foundation
- jQuery
- Leaflet
- Lightbox
- Modernizr
- Moment.js
- Underscore.js
- Video.js
- Vue.js
- Zoom.js
Emails and Hosting {โ๏ธ}
Mail Servers:
- mx.zoho.eu
- mx2.zoho.eu
- mx3.zoho.eu
Name Servers:
- josh.ns.cloudflare.com
- zita.ns.cloudflare.com
CDN Services {๐ฆ}
- Cloudflare
- Crossref