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Nypa fruticans (Arecaceae) is the only monocot species of true mangroves. This species represents the earliest mangrove fossil recorded. How N. fruticans adapts to the harsh and unstable intertidal zone is an interesting question. However, the 60 gene segments deposited in NCBI are insufficient for solving this question. In this study, we sequenced, assembled and annotated the transcriptome of N. fruticans using next-generation sequencing technology. A total of 19,918,800 clean paired-end reads were de novo assembled into 45,368 unigenes with a N50 length of 1,096 bp. A total of 41.35% unigenes were functionally annotated using Blast2GO. Many genes annotated to “response to stress” and 15 putative positively selected genes were identified. Simple sequence repeats were identified and compared with other palms. The divergence time between N. fruticans and other palms was estimated at 75 million years ago using the genomic data, which is consistent with the fossil record. After calculating the synonymous substitution rate between paralogs, we found that two whole-genome duplication events were shared by N. fruticans and other palms. These duplication events provided a large amount of raw material for the more than 2,000 later speciation events in Arecaceae. This study provides a high quality resource for further functional and evolutionary studies of N. fruticans and palms in general.
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Keywords {🔍}
fruticans, article, view, google, scholar, palms, species, pmid, pubmedncbi, unigenes, genes, data, events, plos, analysis, dactylifera, table, sequences, palm, divergence, time, gene, genome, wgd, fig, nypa, transcriptome, high, selected, duplication, guineensis, image, annotation, assembly, sequencing, positively, nucifera, results, total, stress, sequence, model, ssrs, novo, mangrove, zhang, study, functional, distribution, plant,
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microsatellite identification tool cookies plos plos global 30μl reaction mixture seasonal climate reduced tropical areas gene expression map climate optimum normal expression level contributed reagents/materials/analysis tools functional genomics research tropical expression level short-read rna-seq data a-glance quality assessment full-length transcriptome assembly sun yat-sen university inter-simple sequence repeat original author improved gene annotation genome-wide duplication events estimate maximum-likelihood phylogenies improved branch-site model accessible data icon comparative genomics supporting information files fast gapped-read alignment protein signature recognition simple sequence repeats protein sequence alignments van de peer mangrove palm nypa de novo assembly pubmed central pmcid gene product attributes long-term evolutionary history transcriptome data elucidates genomics simple sequence repeat de novo assembled research articles qualify sequence read archive mangrove palm genome duplication events save selected preferences million years ago vivipary seeding–related genes annotated transcriptome data high quality resource
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