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DOI . ORG {}

Detected CMS Systems:

  1. Analyzed Page
  2. Matching Content Categories
  3. CMS
  4. Monthly Traffic Estimate
  5. How Does Doi.org Make Money
  6. Wordpress Themes And Plugins
  7. Keywords
  8. Topics
  9. Social Networks
  10. External Links
  11. Analytics And Tracking
  12. Libraries
  13. Hosting Providers
  14. CDN Services

We began analyzing https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091415, but it redirected us to https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0091415. The analysis below is for the second page.

Title[redir]:
No title found...
Description:
Besnoitia besnoiti infection in cattle is an important emerging protozoan disease in Europe causing economic losses and severe clinical signs, such as generalized dermatitis, orchitis, and vulvitis in affected animals. Neutrophil extracellular trap (NET) formation was recently demonstrated as an important effector mechanism of PMN acting against several invading pathogens. In the present study, interactions of bovine PMN with tachyzoites of B. besnoiti were investigated in this respect in vitro. For the demonstration and quantification of NETs, extracellular DNA was stained by Sytox Orange or Pico Green. Fluorescent illustrations as well as scanning electron microscopy analyses (SEM) showed PMN-promoted NET formation rapidly being induced upon contact with B. besnoiti tachyzoites. Co-localization of extracellular DNA with histones, neutrophil elastase (NE) and myeloperoxidase (MPO) in parasite entrapping structures confirmed the classical characteristics of NET. Exposure of PMN to viable, UV attenuated and dead tachyzoites showed a significant induction of NET formation, but even tachyzoite homogenates significantly promoted NETs when compared to negative controls. NETs were abolished by DNase treatment and were reduced after PMN preincubation with NADPH oxidase-, NE- and MPO-inhibitors. Tachyzoite-triggered NET formation led to parasite entrapment as quantitative assays indicated that about one third of tachyzoites were immobilized in NETs. In consequence, tachyzoites were hampered from active invasion of host cells. Thus, transfer of tachyzoites, previously being confronted with PMN, to adequate host cells resulted in significantly reduced infection rates when compared to PMN-free infection controls. To our knowledge, we here report for the first time B. besnoiti-induced NET formation. Our results indicate that PMN-triggered extracellular traps may represent an important effector mechanism of the host early innate immune response against B. besnoiti which may lead to diminishment of initial parasite infection rates during the acute infection phase.

Matching Content Categories {📚}

  • Science
  • Telecommunications
  • Education

Content Management System {📝}

What CMS is doi.org built with?


Doi.org is based on WORDPRESS. But there are also traces of other content systems on the page (hubspot).

Traffic Estimate {📈}

What is the average monthly size of doi.org audience?

🌍 Impressive Traffic: 500k - 1M visitors per month


Based on our best estimate, this website will receive around 600,019 visitors per month in the current month.
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How Does Doi.org Make Money? {💸}

We're unsure if the website is profiting.

Not all websites are made for profit; some exist to inform or educate users. Or any other reason why people make websites. And this might be the case. Doi.org might be cashing in, but we can't detect the method they're using.

Wordpress Themes and Plugins {🎨}

What WordPress theme does this site use?

It is strange but we were not able to detect any theme on the page.

What WordPress plugins does this website use?

It is strange but we were not able to detect any plugins on the page.

Keywords {🔍}

tachyzoites, pmn, net, formation, besnoiti, article, min, bovine, view, google, scholar, nets, extracellular, cells, parasite, structures, cell, fig, host, neutrophil, mpo, dna, infection, image, significant, plos, traps, tachyzoite, parasites, performed, control, entrapment, ratio, exposure, time, positive, zymosan, incubation, fluorescence, significantly, incubated, apicomplexan, besnoitia, controls, treatment, bovis, samples, immune, induced, dnase,

Topics {✒️}

chromogenic substrate meosuc-ala-ala-pro-val-chloromethyl-ketone cookies plos adequate conjugate systems plos cell biology open-access article distributed protozoan infections contributed reagents/materials/analysis tools poly-l-lysine-treated coverslips established quantitative parasite-entrapment-assays enzyme complex picogreen-derived fluorescence intensities cross-sectional serological survey increases l-selectin shedding original author infections rates estimated tachyzoite-triggered net formation granula-derived neutrophil elastase kinetic analyses revealed tachyzoite-induced net formation parasite-induced net formation net-triggered parasite-entrapment chromogenic substrate tachyzoite-induced net structures infected endothelial cells besnoiti-induced net formation besnoiti-induced net-formation net-triggering molecules originating pmn-triggered extracellular traps exhibit detrimental/lethal effects scanning electron microscopy besnoiti infections pmn-mediated immune reactions protozoan parasites besnoiti-triggered net production tachyzoite-exposed pmn revealing pmn-derived net structures enhanced pmn adhesion besnoiti tachyzoites net-formation host cell invasion poly-l-lysine bovine endothelial cells innate host responses ligated umbilical vein zymosam-pre-activated pmn earliest immune cells enhanced fluorescence intensities parasite-induced netosis experiments fluorescence intensity derived evaluate dose-dependent effects

External Links {🔗}(191)

Analytics and Tracking {📊}

  • Facebook Pixel
  • Google Analytics
  • Google Analytics 4
  • Google Tag Manager
  • Google Universal Analytics
  • HubSpot

Libraries {📚}

  • Foundation
  • jQuery
  • Leaflet
  • Lightbox
  • Modernizr
  • Moment.js
  • Underscore.js
  • Video.js
  • Vue.js
  • Zoom.js

Emails and Hosting {✉️}

Mail Servers:

  • mx.zoho.eu
  • mx2.zoho.eu
  • mx3.zoho.eu

Name Servers:

  • josh.ns.cloudflare.com
  • zita.ns.cloudflare.com

CDN Services {📦}

  • Cloudflare
  • Crossref

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