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The transcriptional effects of the ovarian hormone progesterone are pleiotropic, and binding to DNA of the nuclear progesterone receptor (PR), a ligand-activated transcription factor, results in diverse outcomes in a range of target tissues. To determine whether distinct patterns of genomic interaction of PR contribute to the cell specificity of the PR transcriptome, we have compared the genomic binding sites for PR in breast cancer cells and immortalized normal breast cells. PR binding was correlated with transcriptional outcome in both cell lines, with 60% of progestin-regulated genes associated with one or more PR binding regions. There was a remarkably low overlap between the PR cistromes of the two cell lines, and a similarly low overlap in transcriptional targets. A conserved PR binding element was identified in PR binding regions from both cell lines, but there were distinct patterns of enrichment of known cofactor binding motifs, with FOXA1 sites over-represented in breast cancer cell binding regions and NF1 and AP-1 motifs uniquely enriched in the immortalized normal line. Downstream analyses suggested that differential cofactor availability may generate these distinct PR cistromes, indicating that cofactor levels may modulate PR specificity. Taken together these data suggest that cell-specificity of PR binding is determined by the coordinated effects of key binding cofactors.
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Keywords {π}
binding, cells, regions, foxa, cell, genes, view, article, figure, google, scholar, expression, gene, regulated, transcriptional, progestin, regulation, breast, progesterone, lines, dna, cancer, sites, receptor, data, pre, shown, identified, transcription, enrichment, analysis, response, motifs, transcripts, compared, motif, factor, chromatin, pres, overlap, plos, number, target, strength, pdf, sequence, httpsdoiorgjournalpones, genomic, line, factors,
Topics {βοΈ}
parent pcdh-cmv-mcs-ef1-copgfp virus c-jun/c-fos/estrogen receptor comparable experimental systems pcdh-cmv-mcs-ef1-copgfp plos genet 5 plos genet 3 national health cookies plos carestream health trends genetics 27 plos global gene profiling medicine enhancer-driven lineage-specific transcription promote androgen-receptor-dependent transcription additional pr+mcf-10a clone global inhibition parent mcf-10a cells open-access article distributed contributed reagents/materials/analysis tools pr regulatory complex histone h3/h4 tetramer ligand-activated transcription factor original author nci-60 drug-screening panel unbiased genome-wide fashion mcf-10a cell line kodak image station detailed genome-wide survey quantitative real-time pcr genome-wide expression profiling large coregulatory complex 300-fold vs37-fold enrichment control pcdh virus gene-specific transcriptional regulation illumina ga-iix sequencer de novo analysis glucocorticoid receptor-regulated promoter progesterone-liganded pr binding pearson regression r-squaredβ=β0 binding region-specific primers cell-specific transcriptional outcomes pcdh-foxa1-gfp vector central hormone-responsive elements pre-cleared org-treated samples cell-specific pr activity transformed normal breast empty pcdh control genome-wide analysis determine cell-specific binding
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