DOI . ORG {
}
Detected CMS Systems:
- Wordpress (1 occurrences)
- Hubspot (1 occurrences)
We began analyzing https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010661, but it redirected us to https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1010661. The analysis below is for the second page.
Title[redir]:
No title found...
Description:
Author summary RNA editing alters genetic information at the RNA-level. The most common type of RNA editing is the conversion of adenosine (A) to guanosine (G), which can lead to protein diversification beyond the genomic DNA blueprint. A-to-G editing is catalyzed by members of the highly conserved ADAR protein family, which bind to specific double-stranded RNA (dsRNA) structures. The ability of ADARs to recode at the mRNA level makes them attractive therapeutic tools. The main challenge is to artificially create an editable dsRNA structure around a defined target and redirect ADAR activity to achieve high on-target editing efficiency. It is therefore of much interest to identify highly potent natural ADARs that could be used to induce high-level editing. Here we developed a novel approach to identify such potent enzymes. Using the baker’s yeast Saccharomyces cerevisiae as a neutral testing ground, we identified two exceptionally active editors: the hummingbird ADAR2, and the mallard-duck ADAR1. We provide evidence that these birds which evolved to live with higher core body temperatures have developed ADAR enzymes that target the temperature sensitive dsRNA structures and would therefore be more potent than other ADARs. Further studies may use this approach to pinpoint additional ADAR enzymes to further broaden their applicability.
Title[redir]:
No title found...
Description:
Author summary RNA editing alters genetic information at the RNA-level. The most common type of RNA editing is the conversion of adenosine (A) to guanosine (G), which can lead to protein diversification beyond the genomic DNA blueprint. A-to-G editing is catalyzed by members of the highly conserved ADAR protein family, which bind to specific double-stranded RNA (dsRNA) structures. The ability of ADARs to recode at the mRNA level makes them attractive therapeutic tools. The main challenge is to artificially create an editable dsRNA structure around a defined target and redirect ADAR activity to achieve high on-target editing efficiency. It is therefore of much interest to identify highly potent natural ADARs that could be used to induce high-level editing. Here we developed a novel approach to identify such potent enzymes. Using the baker’s yeast Saccharomyces cerevisiae as a neutral testing ground, we identified two exceptionally active editors: the hummingbird ADAR2, and the mallard-duck ADAR1. We provide evidence that these birds which evolved to live with higher core body temperatures have developed ADAR enzymes that target the temperature sensitive dsRNA structures and would therefore be more potent than other ADARs. Further studies may use this approach to pinpoint additional ADAR enzymes to further broaden their applicability.
Matching Content Categories {📚}
- Science
- Photography
- Video & Online Content
Content Management System {📝}
What CMS is doi.org built with?
Doi.org is powered by WORDPRESS.
But there are also traces of other content systems on the page (hubspot).
Traffic Estimate {📈}
What is the average monthly size of doi.org audience?
🏙️ Massive Traffic: 50M - 100M visitors per month
Based on our best estimate, this website will receive around 76,079,999 visitors per month in the current month.
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How Does Doi.org Make Money? {💸}
We find it hard to spot revenue streams.
Not every website is profit-driven; some are created to spread information or serve as an online presence. Websites can be made for many reasons. This could be one of them. Doi.org might have a hidden revenue stream, but it's not something we can detect.
Wordpress Themes and Plugins {🎨}
What WordPress theme does this site use?
It is strange but we were not able to detect any theme on the page.
What WordPress plugins does this website use?
It is strange but we were not able to detect any plugins on the page.
Keywords {🔍}
editing, rna, adar, article, view, google, scholar, pmid, pubmedncbi, sites, temperature, adars, yeast, mdadar, fig, growth, enzymes, cells, atoi, structures, expression, strains, hadar, dsrna, body, genome, samples, control, temperatures, activity, protein, plos, potent, number, hbadar, structure, human, levels, adenosine, image, detected, high, results, heterologous, enzyme, mismatches, methods, active, cell, effect,
Topics {✒️}
usp=share_link&ouid=102710005033051837999&rtpof=true&sd=true digital image term=reuter+js%2c+mathews+dh plos genet 19 plos genet health cookies plos genome biology bmc genomics es/cgi-bin/nvenn/nvenn medicine global editing adar-derived sequencing reads +rna+secondary+structure+prediction+ complex behavior adar-based systems specific double-stranded rna l-alanyl-l-glutamine 5m tris-cl ph6 earliest-diverging eumetazoan phyla angewandte chemie—international edition raw p-values reported apobec3a-cas9 base editor site-directed rna editing complex tecan microplate reader double-stranded rna structures target single-nucleotide variants high-throughput mutagenesis method original author crispr-cas9 gene editing rna secondary structure rna editing—immune protector rna-seq analysis identifies adar-based base-editors base-line noise level penicillin-streptomycin-nystatin solution rna-sequencing libraries empty ura3-marked plasmid de-novo sites reported large-scale automated annotation dsrna secondary structure single-site genome editing mammalian systems editable dsrna structure supporting information files adar-naïve yeast genome adar-mediated rna-editing target dsrna structure detect mismatches de-novo
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