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Author Summary Rett syndrome (RTT) is a debilitating neurodevelopmental disorder with no cure or effective treatment. To fully understand the disease mechanism and develop therapies, it is necessary to study all aspects of the molecular function of methyl-CpG binding protein 2 (MeCP2), mutations in which have been identified as the genetic cause of RTT. Over the years, MeCP2 has been shown to maintain DNA methylation, regulate transcription and chromatin structure, control microRNA processing, and modulate RNA splicing. Among these known functions, the role of MeCP2 in modulating RNA splicing is less well understood. We took several unbiased approaches to investigate the how MeCP2 may regulate splicing, what splicing changes are caused by the loss of MeCP2, and what functional consequences are caused by altered splicing. We discovered that MeCP2 interacts with splicing factors to regulated the splicing of glutamate receptor genes, which mediate the vast majority of excitatory synaptic transmission in the brain; and linked the altered splicing of glutamate receptor genes to specific synaptic changes in a RTT mouse model. Our findings not only advance the understanding of RTT disease mechanism, but also reveal a potential drug target for future development of therapies.
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Keywords {🔍}
mecp, splicing, gria, article, view, pmid, pubmedncbi, google, scholar, fig, mice, flipflop, analysis, data, cortex, mouse, genes, epub, central, pubmed, pmcid, brain, rna, alternative, gene, ledgf, identified, exon, rtt, proteins, study, flip, interaction, neurons, events, protein, cells, chipseq, exons, pmc, altered, flop, dna, sem, genome, cell, ratio, synaptic, chromatin, western,
Topics {✒️}
human health cell biology aav-esf-flop-gly-mcherry significantly altered swi/snf chromatin-remodeling complex plos genet 12 plos genet cookies plos myc-tagged full-length ledgf/p52 n-terminal pwwp-cr1 domain plos esf-flop-gly expressing neurons inherited mental retardation esf-flop-gly [flop suppressor] methyl-cpg-binding protein methyl-cpg binding protein-2 esf-flop-sr [flop enhancer] esf-flip-gly [flip suppressor] esf-flip-sr [flip enhancer] human molecular genetics mek/erk-regulated cell proliferation methyl-cpg-binding domain antibody-proteing-bead complex expresses flag-tagged mecp2 esf-flop-gly significantly increased glutamate-evoked ampar-gated current als-linked proteins fus/tls swi/snf complex regulates pre-mrna splicing esf-flop-gly didn overexpressing esf-flop-gly conducted high-throughput sequencing global epigenomic reconfiguration aav-esf-flop-gly nature genetics esf-flop-gly lentivirus original author histone deacetylase complex global splicing alterations processing long pre-mrnas li-cor odyssey imager ledgf/p75-binding abilities mecp2-flag knockin mice specific protein-protein interactions reversing flip/flop splicing spinal muscular atrophy flip/flop splicing defect dna methylation data alternative pre-mrna splicing maintain dna methylation maintaining dna methylation[10]
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