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Description:
The highly conserved translational control protein Dhh1 promotes mRNA decapping by regulating a late step in translation in yeast.
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Keywords {🔍}
mrna, dhh, translation, cells, figure, decapping, reporter, protein, article, rna, view, google, scholar, initiation, function, ribosomes, ribosome, tethered, decay, mgfp, pgk, translational, polyribosomes, analysis, mrnas, repression, dhhms, expressing, cell, association, wildtype, data, blot, levels, control, complex, sucrose, eife, binding, min, yeast, elongation, mfa, step, observed, plos, biol, consistent, proteins, gfp,
Topics {✒️}
plos biol 10 plos biol cookies plos medicine phenol/chloroform extraction mental retardation protein contributed reagents/materials/analysis tools chloroform extraction open-access article distributed glutamine/asparagine-rich domain c-terminally tagged version chromosomally zz-tagged version diauxic shift-dependent relocalization messenger ribonucleoprotein complexes atp-dependent rna helicase messenger rnp assembly epitope-tagged dhh1 protein rna-mediated gene silencing strong stem-loop engineered reaching mid-log phase 32p end-labeled oligonucleotides dead-box protein dhh1p original author agarose-formaldehyde gel electrophoresis 48s complex formation wild-type cells shows translating messenger rnas wild-type cells harboring regulating translation post-initiation c-terminal ha tag nonsense-mediated mrna decay wild-type cells expressing translation initiation complex northern blot analysis messenger rna dead box helicase pgk1-ha protein chimera mrna half-lives observed cytoplasmic rnp complex undergoing translation initiation northern blot data dhh1-ms2 versus ms2 ethidium bromide staining dcp2δ rpl16a-zz cells dead-box motif hbht-dhh1 remained predominantly entire eif3 complex dhh1-ms2 represents ribosome dhh1-mediated mrna decay processing body assembly
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