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The screen suggested functions for 393 genes for which no RNAi-mediated phenotype was known. The comparison with similar screens in worms has general implications for RNAi experiments.
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Keywords {🔍}
rnai, rrf, phenotypes, genes, phenotype, clones, genetic, mutants, detected, elegans, fraser, screen, gene, screens, strain, kamath, table, article, view, found, plos, results, data, google, scholar, rna, mutant, unc, chromosome, experiments, genomewide, wildtype, differences, dsrna, postembryonic, compared, addition, detection, confirmed, dpye, interference, library, increased, lethality, caenorhabditis, image, laboratory, number, obtained, defects,
Topics {✒️}
plos biol 1 biology cookies plos public library specific rna-mediated interference rna-directed rna polymerase facilitate rapid cloning open-access article distributed science open-access license genetics genetics 113 positional cloning systematic rna interference systems experimentally ingested double-stranded rna isopropylthio-β-d-galactoside uk/projects/c_elegans/blast_server global rnai screens cloning dsrna-triggered gene silencing systems specific genetic interference rna interference caenorhabditis elegans genome wild-type bristol n2 genome-wide rnai analysis double-stranded rna large-scale screens increases nematode caenorhabditis elegans large-scale rnai screens global scale genome-wide rnai screen genome-wide rrf-3 dataset original rnai protocol gene clustering based genome-wide screens targeted gene silencing give variable results global screen inhibit gene function vivo gene function slow-growth mutant phenotype standard laboratory strain genetic mutants display specific interference give transient loss gene functions genome-wide rnai wild-type worms dominant movement defects
Questions {❓}
- What could be the source of the interexperimental variation of RNAi?
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