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Author summary Newborn piglets are particularly susceptible to infection by PEDV, with 80โ100% dying within days of infection. The reasons for newbornsโ acute susceptibility to PEDV infection have not been elucidated clearly. The primarily target of PEDV is the porcine intestinal epithelial cells. Here, we show that the high expression of TfR1 in the apical tissue of intestinal villi in newborn piglets with iron deficiency is a reason for their susceptibility to PEDV. Further, we demonstrate that iron supplementation reduces PEDV infection. This study reveals that iron plays an important role in the susceptibility of newborn piglets to PEDV and provides insights into therapies for the prevention and treatment of PEDV infections.
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Keywords {๐}
pedv, tfr, cells, infection, piglets, iron, fig, article, view, google, scholar, pmid, pubmedncbi, virus, newborn, protein, western, infected, intestinal, porcine, expression, ipecj, dfo, fac, receptor, levels, entry, pbs, cell, diarrhea, vero, pigs, group, treated, epidemic, epithelial, src, blotting, journal, results, deficiency, jejunum, transferrin, membrane, pedvn, antitfr, antibody, experiments, flow, cytometry,
Topics {โ๏ธ}
human gastrointestinal tract fusion core complex gastrointestinal tract plos pathog 16 cookies plos plos iron-responsive element/iron-regulatory protein goat anti-rabbit igg-hrp human health health guidelines hrp-conjugated secondary antibodies supporting information files fluorochrome-conjugated secondary antibodies original author species-specific virus host-range tfr1-silenced ipec-j2 cells free radical biology mouse monoclonal anti-p-tyr nature chemical biology allogeneic anti-rabbit igg serial ten-fold dilutions preventive veterinary medicine clathrin-coated pit internalization anti-rabbit igg antibody emerging infectious diseases ice-cold sterile saline 649-conjugated anti-tfr1 antibody hiv-infected tanzanian women porcine epidemic diarrhea colostrum-deprived xinhuai piglets cold phosphate-buffered saline bactericidal/permeability-increasing protein fine-tuning hepcidin expression polarized ipec-j2 cells rabbit anti-tfr1 pab cell biology commensalโhost immune crosstalk hiv-positive individuals suffer tfr1-silenced vero cells mouse monoclonal anti-pedv rabbit monoclonal anti-gapdh bd cytofix/cytoperm kit cellular biology ipec-j2 cells pretreated clathrin-mediated tfr1 internalization rabbit anti-tfr1 ab ipec-j2 cells treated 100 ฮผg/ml penicillin/streptomycin mouse monoclonal antibodies iron-loaded transferrin binds
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