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Angiogenesis in glioma is associated with the poor prognosis of the disease and closely correlates with the highly invasive phenotype of glioma cells, which represents the most challenging impediment against the currently glioma treatments. Bmi-1, an onco-protein, has been implicated in the progression of various human cancers, including gliomas, whereas its role in glioma angiogenesis remains unclear. Our current study examined the effects of Bmi-1 on glioma angiogenesis in vitro as well as in vivo. We found that overexpression of Bmi-1 enhanced, whereas knockdown of Bmi-1 diminished, the capability of glioma cells to induce tubule formation and migration of endothelial cells and neovascularization in chicken chorioallantoic membrane. In vivo, Bmi-1 overexpression and knockdown, respectively, promoted and inhibited angiogenesis in orthotopically transplanted human gliomas. Furthermore, NF-κB activity and VEGF-C expression was induced by Bmi-1 overexpression, whereas Bmi-1 knockdown attenuated NF-κB signaling and decreased VEGF-C expression. Additionally suppression of NF-κB activity using a specific chemical inhibitor abrogated the NF-κB activation and the pro-angiogenic activities of glioma cells. Together, our data suggest that Bmi-1 plays an important role in glioma angiogenesis and therefore could represent a potential target for anti-angiogenic therapy against the disease.
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Keywords {🔍}
bmi, cells, glioma, angiogenesis, expression, article, view, vegfc, google, scholar, nfκb, figure, gliomas, cancer, tumor, cell, signaling, huvec, plos, human, activity, control, assay, vitro, induced, promotes, brain, performed, bmioverexpressing, data, image, progression, role, migration, genes, usa, experiments, quantification, formation, analysis, levels, images, vivo, independent, previously, vector, vectorcontrol, protein, left, mrna,
Topics {✒️}
pi-3k/akt/gsk-3β pathway [14] health open-access article distributed nasopharyngeal carcinoma cells cookies plos nasopharyngeal carcinoma progression plos digital camera ikk-nuclear factor-kappab pathway contributed reagents/materials/analysis tools cancer biology primary brain tumors medicine cancer-related signaling pathways astroglial brain tumors breast cancer activating nf-κb signaling nf-κb signaling pathway nf-κb-regulated gene expression psuper-retro-puro-vector induces epithelial-mesenchymal transition nf-κb binding site lipopolysaccharide-induced nuclear translocation nf-kappab motif drives real-time rt-pcr biology releasing matrix-bound vegf enzyme-linked immunosorbent assay acute myeloid leukemia real-time pcr analysis identify diagnostic/prognostic biomarkers prl-tk renilla plasmid nf-κb transcriptional activity matrix metalloproteinase-9 triggers original author methods ethics statement lj ml s-yc breast bmi-1-induced glioma angiogenesis real-time pcr quantification nf-κb signaling nf-κb target genes genetics bmi-1-silenced glioma cells bmi-1-specific rna interference bmi-1-overexpressing glioma cells bmi-1-silencing glioma cells functional nf-κb activation mu-myc transgenic mice brain glioma-bearing mice
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