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Author summary Programmed cell death (PCD) has crucial roles in development and immunity in multicellular organisms. In plants, rapid PCD induction, so-called hypersensitive response (HR) cell death, can be triggered as a part of immune system, and plays an important role in restricting pathogen growth. Despite its importance, cell death induction can backfire on plants because of the diversified infection strategies of plant pathogens. It is therefore assumed that plants have mechanisms by which they are able to minimize PCD induction during plant-pathogen interactions. However, their existence and biological significance are not clear yet. Here, we demonstrate that PSIG1, which has the GYF domain that is highly conserved among diverse eukaryotic species, restricts cell death induction during pathogen invasions. Importantly, psig1 mutants do not display autoimmune phenotypes, and are more susceptible to the virulent bacterial pathogen. Our findings suggest that the restriction of cell death can have benefits for plants to defend themselves against hemi-biotrophic bacterial pathogen infections. We further provide evidence suggesting a mechanism by which PSIG1 may contain cell death by regulating the RNA metabolism machinery.
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Keywords {π}
psig, cell, death, fig, plants, article, view, google, scholar, plant, mutants, pmid, pubmedncbi, pto, gyf, mutant, smg, blue, domain, induction, arabidopsis, cells, leaves, trypan, inoculation, inoculated, data, pathogen, determined, infection, phenotype, proteins, stained, dead, bacterial, test, days, pathogens, avrrps, hpa, shown, growth, enhanced, gene, spray, cfu, bak, expression, statistical, differences,
Topics {βοΈ}
proline-rich sequence proline-proline-glycine-phenylalanine tir-nb-lrr-type receptor proteins plos genet 13 plos genet cookies plos plos flagellin-induced complex alternative nb-lrr-type receptors brassinosteroid-independent cell-death pathways nonsense-mediated rna decay hemi-biotrophic bacterial pathogens rps4-triggered cell death recognizes proline-rich sequences hemi-biotrophic bacterial pathogen temperature-dependent cell death rrs1/rps4-triggered eti [49] pathogen-triggered cell death hemi-biotrophic pathogens switch eti-triggered cell death t-dna insertion sites proline-rich sequence recognition full-length psig1 interacted pseudomonas syringae pv avrrpm1-triggered cell death agrobacterium carrying pjl3-p19 avrrps4-triggered cell death early pamp-responsive phosphoprotein plant cell-death control c-terminally truncated psig1 t-dna insertion site 8-mm-diameter cork borer psig1-dependent cell death early pamp-responsive protein original author cell death-triggering mechanisms avirulent bacterial pathogens stop codon-mutated psig1pro pseudomonas syringae effector lactophenol-trypan blue solution reactive oxygen intermediates downy mildew fungus semi-quantitative rt-pcr hemi-biotrophic pathogens hemi-biotrophic pathogens [2] ile-type gyf domains agonist-dependent gain control pentr4 dual-selection vector label-free quantitative proteomics conserved Ξ²-hairpin recognition flg22-induced stomatal closure
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- To die or not to die?
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