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Author Summary Mitochondria play a crucial role in metabolic homeostasis, and alteration of mitochondrial function is a hallmark of diabetes. While mitochondrial activity is reduced in people with diabetes, it is unclear whether mitochondrial dysfunction is a cause or effect of type 2 diabetes. Genome-wide association studies for type 2 diabetes have explained ≈10% of the heritability of the disease, but none of the loci are known to affect mitochondrial activity. It is possible though that a mitochondrial contribution is hidden in the remaining 90%. Hence, we tested the hypothesis that multiple mitochondria-related genes encoded in the nucleus, each having a weak effect (hard to detect individually), can collectively influence type 2 diabetes. To address this, we developed a computational method (MAGENTA) that allowed us to adequately analyze large collective datasets of human genetic variation obtained from collaborative studies of type 2 diabetes and related glycemic traits. Despite the increased sensitivity of MAGENTA compared to single-DNA variant analysis, we found no support for a causal relationship between mitochondrial dysfunction and type 2 diabetes. These results may help steer future efforts in understanding the pathogenesis of the disease. MAGENTA is broadly applicable to testing associations between other biological pathways and common diseases or traits.
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Keywords {🔍}
gene, genes, set, association, analysis, mitochondrial, sets, gwa, correction, snps, snp, enrichment, pvalues, gsea, table, study, size, diabetes, magenta, significant, article, metaanalysis, power, view, associations, google, scholar, genome, regression, score, effect, pvalue, tested, figure, data, studies, dgi, effects, genetic, scores, confounders, causal, methods, number, method, traits, individuals, test, metaanalyses, genomewide,
Topics {✒️}
_ob=articleurl&_udi=b7mfh-4rj518m-8&_user=209690&_coverdate=01%2f31%2f2008&_alid=1179716171&_rdoc=1&_fmt=high&_orig=search&_cdi=23259&_st=1&_docanchor=&_ct=14&_acct=c000014438&_version=1&_urlversion=0&_userid=209690&md5=d8d4b7c3bc5ff26bd8514308c4d89cd5 health common single-nucleotide polymorphisms plos genet 6 plos genet 4 cookies plos single-nucleotide polymorphisms plos molecular biology reports pgc-1alpha-responsive genes involved step-wise linear regression computational method genome-wide association meta-analysis genome-wide association meta-analyses computational advice permutation-corrected gene p-values contributed reagents/materials/analysis tools step-wise regression analysis underlying biology uk/pub/programs/ldmap [57] tailed rank-sum p disease biology meta-analysis gene-set enrichment reduces palmitate-induced apoptosis open-access article distributed genome-wide association studies large genome-wide association van vliet-ostaptchouk jv expression-qtls yields insight biology future genome-wide maps linear regression-based approach original author comprehensive genome-wide analysis genome-wide association study genome-wide association data genome-wide association test genome-wide association signals latest t2d meta-analysis mitochondria-related gene sets chi-square test statistic gwa scan meta-analyses gene association p-values single-dna variant analysis genome-wide pathway analysis permutation-corrected gene scores triglyceride gwa meta-analysis insulin-related traits characteristic diagram+ t2d meta-analysis noncentral chi-square distribution
Questions {❓}
- (2009) Mitochondria and diabetes mellitus: untangling a conflictive relationship?
- Dumas J, Simard G, Flamment M, Ducluzeau P, Ritz P (2009) Is skeletal muscle mitochondrial dysfunction a cause or an indirect consequence of insulin resistance in humans?
- Turner N, Heilbronn LK (2008) Is mitochondrial dysfunction a cause of insulin resistance?
- Edu/science?
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